【摘要】：目的探討正常和熱應(yīng)激條件下,葡萄糖轉(zhuǎn)運(yùn)蛋白1(GLUT1)和葡萄糖轉(zhuǎn)運(yùn)蛋白2(GLUT2)在成年豬睪丸的表達(dá)和定位。方法性成熟長白公豬9頭,隨機(jī)分為3組。局部陰囊熱刺激組(n=3),用自制電熱毯置陰囊42℃加熱1 h;環(huán)境熱應(yīng)激組(n=3),每天置于37~40℃豬舍環(huán)境3 h,連續(xù)7 d,每天于熱處理結(jié)束后,將實(shí)驗(yàn)豬驅(qū)趕回21~25℃豬舍環(huán)境;對照組(n=3),飼養(yǎng)在21~25℃豬舍環(huán)境。局部熱刺激6 h后和環(huán)境熱應(yīng)激處理結(jié)束24 h后,手術(shù)摘除雙側(cè)睪丸。用Real-time PCR、Western blotting和免疫組織化學(xué)技術(shù)檢測豬睪丸組織內(nèi)GLUT1和GLUT2的表達(dá)。結(jié)果 Real-time PCR和Western blotting結(jié)果顯示,與對照組相比,環(huán)境熱應(yīng)激組GLUT1蛋白和mRNA的表達(dá)差異不顯著,局部陰囊熱刺激組GLUT1蛋白和mRNA表達(dá)顯著升高;環(huán)境熱應(yīng)激組和局部陰囊熱刺激組,GLUT2蛋白和mRNA表達(dá)均顯著升高。免疫組織化學(xué)結(jié)果發(fā)現(xiàn),熱處理前后,GLUT1蛋白在曲精小管內(nèi)定位于精母細(xì)胞和圓形精子細(xì)胞;環(huán)境熱應(yīng)激組GLUT1蛋白染色與對照組相比,無明顯差異,局部陰囊熱刺激后,GLUT1染色變深,表達(dá)升高。熱處理前后,GLUT2蛋白在曲精小管定位于生精細(xì)胞和支持細(xì)胞,環(huán)境熱應(yīng)激和局部陰囊熱刺激導(dǎo)致GLUT2染色變深,表達(dá)升高。結(jié)論葡萄糖轉(zhuǎn)運(yùn)蛋白GLUT1和GLUT2表達(dá)于豬睪丸曲精小管,環(huán)境高溫和陰囊局部熱刺激導(dǎo)致GLUT1和GLUT2在豬睪丸的表達(dá)水平改變,提示這兩種葡萄糖轉(zhuǎn)運(yùn)蛋白在豬精子發(fā)生過程中發(fā)揮重要作用。
[Abstract]:Objective to investigate the expression and localization of glucose transporter 1 (GLUT1) and glucose transporter 2 (GLUT2) in adult pig testis under normal and heat stress. Methods 9 adult Landrace boars were randomly divided into 3 groups. In the local scrotal heat stimulation group (nc3), the scrotum was heated at 42 鈩，

本文編號：2209367