【摘要】：精液的長期保存通常采用冷凍保存,該技術(shù)在牛上已實(shí)現(xiàn)了商業(yè)化,但是山羊的精液冷凍效果卻不理想,受精率和妊娠率較低,因此主要以液態(tài)保存為主。研究中發(fā)現(xiàn),山羊精液液態(tài)保存至12天與保存16天時(shí)活率均能達(dá)到40%左右,但利用保存12天和16天的精液人工授精母羊的受胎率卻出現(xiàn)顯著差異,有必要進(jìn)一步研究和分析母羊的受胎率差異的原因,為進(jìn)一步提高山羊精液液態(tài)保存方案奠定基礎(chǔ)。本實(shí)驗(yàn)利用mZAP的改進(jìn)液MES稀釋液,添加20%的卵黃包被,然后再用MES稀釋液保存精子,研究不同保存時(shí)間精子活力和存活率、精子質(zhì)膜和頂體完整性、精液稀釋液中氧化還原狀態(tài)的變化以及對(duì)保存12天和15天的精液進(jìn)行人工授精,結(jié)果如下：(1)精子的活力和存活率隨著保存時(shí)間的延長而逐漸下降,其中活力在第14天下降幅度最大,存活率在第15天顯著降低；(2)精子質(zhì)膜完整性在第14天顯著下降,頂體完整性從第15開始有顯著下降；(3)精液稀釋液中的氧化還原狀態(tài)隨保存時(shí)間的延長也發(fā)生變化,TOS含量逐漸升高,第14天變化幅度最大；還原態(tài)也是隨著保存時(shí)間的延長而升高,但從第14天開始,還原態(tài)開始下降。第14-16天精子的OSI的上升幅度達(dá)到最大；(4)保存12天的精液人工授精后受胎率與對(duì)照組無顯著差異,但保存15天組受胎率顯著降低。上述結(jié)果表明,山羊精液液態(tài)保存過程中,精子活力、存活率、質(zhì)膜完整、頂體完整都會(huì)隨保存時(shí)間的延長而逐漸下降,保存12天的精液具有與對(duì)照組幾乎相當(dāng)?shù)氖芴ヂ?但保存15天精液受胎率明顯下降。
[Abstract]:Cryopreservation is usually used in the long-term preservation of semen, which has been commercialized on cattle. However, the freezing effect of goat semen is not ideal, the fertilization rate and pregnancy rate are low, so liquid storage is the main method. It was found that the survival rate of goat semen stored in liquid state for 12 days and 16 days could reach about 40%, but the pregnancy rate of artificial insemination ewes with 12 and 16 days of preservation was significantly different. It is necessary to further study and analyze the reasons for the difference of conception rate of ewe, so as to lay a foundation for further improving the plan of liquid preservation of goat semen. In this experiment, the modified MES diluent of mZAP was added with 20% egg yolk coating, then the sperm was preserved with MES diluent. The sperm motility and survival rate, the integrity of plasma membrane and acrosome were studied. The changes of redox state in semen diluent and artificial insemination of semen stored for 12 and 15 days were as follows: (1) the viability and survival rate of spermatozoa decreased with the prolongation of preservation time. The motility of spermatozoa decreased significantly on the 14th day and the survival rate decreased significantly on the 15th day. (2) the sperm plasma membrane integrity decreased significantly on the 14th day, and the acrosomal integrity decreased significantly from the 15th day. (3) the redox state of semen dilute also changed with the prolongation of storage time, and the content of TOS increased gradually, and the biggest change was on the 14th day, and the redox state also increased with the prolongation of preservation time, but from the 14th day, the content of TOS increased. The reduced state began to decrease. (4) there was no significant difference between the pregnancy rate of the semen after 12 days of preservation and the control group, but the pregnancy rate of the 15 days group was significantly lower than that of the control group. The results showed that the sperm motility, survival rate, plasma membrane integrity and acrosome integrity decreased with the prolongation of preservation time during liquid preservation of goat semen, and the semen stored for 12 days had almost the same conception rate as the control group. However, the pregnancy rate of semen decreased significantly after 15 days of preservation.
【學(xué)位授予單位】：山東農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S827.3

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