【摘要】：為研究新疆阿魏多糖(FSP)對(duì)雞外周血T淋巴細(xì)胞和脾臟B淋巴細(xì)胞增殖作用的影響。本研究用水提醇沉法得到阿魏總多糖(FSPt)和3種分級(jí)多糖(FSP30、FSP50、FSP70),并通過(guò)苯酚—硫酸法和紅外光譜對(duì)FSP進(jìn)行檢測(cè)。通過(guò)MTT法測(cè)定各級(jí)FSP的安全濃度及其對(duì)雞外周血T淋巴細(xì)胞及脾臟B淋巴細(xì)胞的增殖能力。結(jié)果顯示,提取的FSP均具有多糖的特征峰,為多糖類(lèi)化合物。外周血T淋巴細(xì)胞增殖試驗(yàn)表明,除FSP50外,所有多糖在一定濃度下均能單獨(dú)和協(xié)同PHA刺激T淋巴細(xì)胞增殖,其中FSPt的增殖作用較強(qiáng)。脾臟B淋巴細(xì)胞增殖試驗(yàn)表明,除FSP50外,所有多糖在一定濃度下均能單獨(dú)和協(xié)同LPS刺激B淋巴細(xì)胞增殖,其中FSP70和FSPt增殖作用較強(qiáng)。綜上所述,各級(jí)FSP中,FSPt對(duì)脾臟B淋巴細(xì)胞和外周血T淋巴細(xì)胞的增殖作用最強(qiáng),可以作為進(jìn)一步研究的材料。
[Abstract]:To study the effect of Xinjiang Ferula polysaccharide (FSP) on the proliferation of T lymphocytes and B lymphocytes in chicken peripheral blood. In this study, the total polysaccharide of ferulic (FSPt) and three kinds of graded polysaccharides (FSP30,FSP50,FSP70) were obtained by water extraction and alcohol precipitation, and FSP was determined by phenol-sulfuric acid method and infrared spectrum. The safe concentration of FSP at all levels and its proliferation ability to T lymphocytes and B lymphocytes in peripheral blood of chicken were determined by MTT method. The results showed that the extracted FSP had the characteristic peak of polysaccharides and was a polysaccharide compound. The T lymphocyte proliferation test in peripheral blood showed that all polysaccharides except FSP50 could stimulate T lymphocyte proliferation alone and in coordination with PHA, and FSPt had stronger proliferative effect. Splenic B lymphocyte proliferation test showed that all polysaccharides except FSP50 could stimulate B lymphocyte proliferation alone and in coordination with LPS. FSP70 and FSPt had stronger proliferative effects. In conclusion, the proliferation of spleen B lymphocytes and peripheral blood T lymphocytes by FSPt was the strongest in all levels of FSP, and could be used as a material for further study.
【作者單位】： 新疆農(nóng)業(yè)大學(xué)動(dòng)物醫(yī)學(xué)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(31360625) 新疆農(nóng)業(yè)大學(xué)研究生創(chuàng)新項(xiàng)目(XJAUGRI2015015)
【分類(lèi)號(hào)】：S831.5

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