發(fā)布時間：2018-07-18 10:37

【摘要】：目的：本研究以綿羊脾臟為制備轉(zhuǎn)移因子(TF)的原料,進行綿羊脾臟轉(zhuǎn)移因子(S-TF)制備方法的探究,明確S-TF的制備原理及制備方法。首先,對S-TF進行初步的理化性質(zhì)分析；其次進行動物試驗,探究S-TF是否會對動物器官產(chǎn)生致病變作用以及是否具有提高動物免疫功能的作用。方法：本研究以相關(guān)文獻資料為依據(jù),采取凍融-透析的方法進行S-TF的制備。制備步驟經(jīng)過反復(fù)摸索確定后,先后開展了S-TF的制備、初步的理化性質(zhì)分析、實驗動物安全性評價和小鼠免疫功能影響的探究。理化性質(zhì)分析包括：外觀性狀、pH值、蛋白質(zhì)反應(yīng)、多肽含量的測定、核糖含量的測定、核苷酸與多肽的比值(OD260/OD280)的測定、氨基酸種類及含量的測定、礦物元素含量的測定、無菌檢驗等。動物試驗分兩部分：第一部分是將粗提的S-TF給小鼠灌服15d,首先眼球采血進行血常規(guī)檢測,其次測定脾臟指數(shù),再次取心臟、肺臟、肝臟、脾臟、腎臟、胃、十二指腸、空腸、回腸、盲腸、結(jié)腸、直腸等器官制作病理切片并進行病理組織學(xué)檢查；第二部分是進行為期19d的小鼠腹腔巨噬細胞吞噬雞紅細胞試驗,測定吞噬百分率和吞噬指數(shù),通過上述兩部分試驗：檢驗S-TF是否具有提高免疫功能的作用。結(jié)果：通過對S-TF制備方法的反復(fù)摸索,已經(jīng)掌握了基本制備原理和方法。通過初步的理化性質(zhì)分析,各項測定結(jié)果均符合TF的國家標準。動物試驗的結(jié)果顯示：試驗組小鼠的白細胞數(shù),特別是中性粒細胞數(shù)目顯著升高；光學(xué)顯微鏡下觀察小鼠主要組織器官無病理變化；脾臟指數(shù)無顯著差異；S-TF可以提高小鼠腹腔巨噬細胞的吞噬百分率和吞噬指數(shù)等結(jié)果,表明S-TF具有提高小鼠的免疫功能的作用。
[Abstract]:Objective: to investigate the preparation method of sheep spleen transfer factor (S-TF) using sheep spleen as the raw material to prepare transfer factor (TF), and to clarify the preparation principle and method of S-TF. First, the physical and chemical properties of S-TF were analyzed, and then the animal experiments were carried out to find out whether S-TF could cause pathological changes in animal organs and improve the immune function of animals. Methods: in this study, S-TF was prepared by freezing-thawing-dialysis. After repeated exploration, the preparation of S-TF, the preliminary analysis of physical and chemical properties, the safety evaluation of experimental animals and the influence of mouse immune function were carried out. Physical and chemical properties include: physical and chemical properties: pH value, protein reaction, determination of polypeptide content, determination of ribosomal sugar content, determination of the ratio of nucleotide to peptide (OD260 / OD280), determination of amino acid type and content, determination of mineral element content. Aseptic examination, etc. The animal experiment was divided into two parts: in the first part, the crude S-TF was infused to mice for 15 days. First, blood was taken from eyeball for routine examination, then spleen index was measured, heart, lung, liver, spleen, kidney, stomach, duodenum, jejunum were taken again. Pathological sections of ileum, cecum, colon and rectum were made and histopathological examination was carried out. The second part was the phagocytosis of chicken erythrocytes by peritoneal macrophages of mice for 19 days, and the phagocytic percentage and phagocytosis index were measured. Through the above two parts of the experiment: to test whether S-TF has the function of improving immune function. Results: the basic preparation principle and method have been mastered through repeated exploration of the preparation method of S-TF. Through the preliminary analysis of physicochemical properties, the results are in accordance with TF national standard. The results of animal experiments showed that the number of white blood cells, especially neutrophils, was significantly increased in the experimental group, no pathological changes were observed in the main tissues and organs under optical microscope, and there was no significant difference in spleen index between the experimental group and the control group. S-TF could increase the phagocytic percentage and phagocytic index of mouse peritoneal macrophages, which indicated that S-TF could improve the immune function of mice.
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S852.4

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