體外法研究纖維日糧的發(fā)酵特性及對豬氮排放相關指標的影響
發(fā)布時間:2018-07-15 12:24
【摘要】:【目的】利用體外法探討并比較不同纖維日糧的發(fā)酵特性及對豬氮排放相關指標的影響,為生豬生產(chǎn)中合理應用日糧纖維及減少氮排放的日糧配制提供試驗依據(jù)!痉椒ā窟x擇小麥、麥麩與燕麥麩3種原料,采用酶-重量法(AOAC Method 985.29)提取原料中的總?cè)占Z纖維組分,得到小麥纖維、麥麩纖維與燕麥麩纖維。以玉米-豆粕為基礎,分別添加5%的提取纖維,形成3種纖維日糧:小麥纖維日糧(WF)、麥麩纖維日糧(WBF)與燕麥麩纖維日糧(OBF)。利用單胃動物仿生消化系統(tǒng)(SDS-Ⅱ)模擬豬胃-小腸消化;采集6頭健康、體重接近的生長豬的新鮮糞便,混合均勻后與培養(yǎng)液按一定比例配制接種液,定量裝入發(fā)酵瓶中模擬大腸發(fā)酵。日糧經(jīng)胃-小腸仿生消化后,稱取200 mg的消化產(chǎn)物于裝有30 m L接種液的發(fā)酵瓶中,轉(zhuǎn)入(39±0.25)℃培養(yǎng)箱中,分別在發(fā)酵4、12、24、36、48和72 h,取出對應發(fā)酵瓶,收集發(fā)酵殘渣與發(fā)酵液,進行相關指標的檢測!窘Y(jié)果】在體外大腸發(fā)酵階段,不同纖維日糧組的干物質(zhì)降解率(IVDMD)及發(fā)酵液短鏈脂肪酸(SCFA)含量、p H、氨氮(NH3-N)濃度、微生物蛋白(MCP)產(chǎn)量有顯著差異(P0.05):(1)在發(fā)酵4與12 h,OBF組的IVDMD在3組中最高(P0.01),其他時間點3組差異不顯著(P0.05)。在4 h,OBF組的IVDMD比WF組與WBF組分別高18.32%、15.21%(P0.01);在12 h,比WF組與WBF組分別高14.87%、10.06%(P0.01)。(2)在發(fā)酵24與36 h,OBF組發(fā)酵液總SCFA含量顯著高于其他兩組(P0.05),在72 h,OBF組與WBF組極顯著高于WF組(P0.01),在4與12h,OBF組有高于其他兩組的趨勢(P=0.0599;P=0.0504)。在24 h,OBF組發(fā)酵液總SCFA含量比WF組與WBF組分別高27.32%、17.11%(P0.05);在36 h,比WF組與WBF組分別高16.65%、25.96%(P0.05)。(3)在發(fā)酵4與12 h,OBF組發(fā)酵液p H在3組中最低(P0.01);在24、36與72 h,OBF組與WBF組顯著低于WF組(P0.05)。(4)在發(fā)酵24與48 h,OBF組發(fā)酵液NH3-N濃度顯著低于其他兩組(P0.05),并在12 h,有低于其他兩組的趨勢(P=0.0559)。在24 h,OBF組發(fā)酵液NH3-N濃度比WF組與WBF組分別低6.86%、4.59%(P0.05);在48 h,比WF組與WBF組分別低8.44%、7.09%(P0.05)。(5)在發(fā)酵4 h,OBF組發(fā)酵液MCP產(chǎn)量顯著高于其他兩組(P0.05),并分別在12、24 h與36、48 h,顯著高于WF組與WBF組(P0.05)。在4 h,OBF組發(fā)酵液MCP產(chǎn)量比WF組與WBF組分別高69.85%、82.25%(P0.05)!窘Y(jié)論】體外條件下,燕麥麩纖維顯著提高了日糧在發(fā)酵階段的干物質(zhì)降解率、發(fā)酵液總短鏈脂肪酸含量與微生物蛋白產(chǎn)量,并顯著降低了發(fā)酵液p H與氨氮濃度。因此,相比小麥纖維與麥麩纖維,燕麥麩纖維在促進微生物發(fā)酵及氮減排方面具有更高的潛力。
[Abstract]:[objective] to study and compare the fermentation characteristics of different fiber diets and their effects on the nitrogen emission of pigs in vitro. [methods] three kinds of raw materials, wheat, wheat bran and oat bran, were selected to extract the total fiber components from the raw materials by enzyme gravimetric method (AOAC method 985.29). Wheat fiber, wheat bran fiber and oat gluten fiber were obtained. On the basis of corn-soybean meal, three fiber diets were formed: wheat fiber diet (WF), wheat gluten fiber diet (WBF) and oat gluten fiber diet (OBF). The bionic digestive system of monogastric animals (SDS- 鈪,
本文編號:2124065
[Abstract]:[objective] to study and compare the fermentation characteristics of different fiber diets and their effects on the nitrogen emission of pigs in vitro. [methods] three kinds of raw materials, wheat, wheat bran and oat bran, were selected to extract the total fiber components from the raw materials by enzyme gravimetric method (AOAC method 985.29). Wheat fiber, wheat bran fiber and oat gluten fiber were obtained. On the basis of corn-soybean meal, three fiber diets were formed: wheat fiber diet (WF), wheat gluten fiber diet (WBF) and oat gluten fiber diet (OBF). The bionic digestive system of monogastric animals (SDS- 鈪,
本文編號:2124065
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