本文選題：蒙古馬 + 耐力訓(xùn)練��； 參考：《內(nèi)蒙古農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：馬業(yè)是古老的產(chǎn)業(yè),而我國(guó)是傳統(tǒng)的養(yǎng)馬國(guó)家,養(yǎng)馬歷史悠久。蒙古馬是我國(guó)數(shù)量最多、分布最廣的馬,具有耐受性強(qiáng)、抗旱、抗寒、抗病等特性,在馬的遺傳資源上是一個(gè)極為寶貴的基因庫(kù)。因此,對(duì)我國(guó)優(yōu)秀地方品種蒙古馬血糖相關(guān)基因進(jìn)行研究,不僅可以進(jìn)一步了解蒙古馬耐力強(qiáng)的原因,為揭示蒙古馬耐力強(qiáng)遺傳特性提供理論依據(jù),而且對(duì)地方品種馬的改良及培育有著重要的意義。本研究以6匹遺傳背景相似,并接受過(guò)為期四個(gè)月運(yùn)動(dòng)訓(xùn)練的蒙古馬為研究對(duì)象。首先,通過(guò)運(yùn)動(dòng)訓(xùn)練對(duì)蒙古馬血液生理生化指標(biāo)進(jìn)行測(cè)定,采用SAS(The system for Windows 9.0)軟件對(duì)蒙古馬訓(xùn)練前后血液生理生化指標(biāo)的基礎(chǔ)數(shù)據(jù)進(jìn)行方差分析。結(jié)果表明,訓(xùn)練前后,血糖、肌酸激酶、尿素、血紅蛋白、血紅細(xì)胞、白細(xì)胞、淋巴細(xì)胞及血小板計(jì)數(shù)均呈現(xiàn)出顯著變化。其中,訓(xùn)練前馬匹血糖濃度為(4.77±0.42) mmol/L,訓(xùn)練后馬匹血糖濃度為(5.83±0.31)mmol/L,高于訓(xùn)練前,前后差異顯著(P0.05)。其次,分別對(duì)訓(xùn)練前后的蒙古馬骨骼肌樣品進(jìn)行了轉(zhuǎn)錄組高通量測(cè)序分析,并對(duì)篩選到的差異基因進(jìn)行GO功能富集,在term條目上,篩選到與血糖相關(guān)的LPHN3、LGALS3.IFIH1基因作為本研究的目的基因。采用實(shí)時(shí)熒光定量RT-qPCR方法檢測(cè)蒙古馬訓(xùn)練前后骨骼肌和血液中LPHN3、LGALS3、IFIH1基因的mRNA表達(dá)量的變化。結(jié)果表明,訓(xùn)練前后,LPHN3、LGALS3、IFIH1三個(gè)基因在蒙古馬血液和骨骼肌中均有表達(dá)。其中,LPHN3基因在訓(xùn)練后蒙古馬骨骼肌中極顯著量高于訓(xùn)練前(P0.01),在訓(xùn)練前后,血液中的表達(dá)量差異不顯著(P≥0.05)。LGALS3基因在訓(xùn)練后蒙古馬骨骼肌和血液中的表達(dá)量均高于訓(xùn)練前,其中骨骼肌中的表達(dá)量差異顯著(P0.05),血液中的表達(dá)差異極顯著(P0.01)。IFIH1基因在訓(xùn)練后蒙古馬血液中的表達(dá)量極顯著高于訓(xùn)練前(P0.01),骨骼肌中表達(dá)量差異顯著(P0.05)。
[Abstract]:Horse industry is an ancient industry, and our country is a traditional horse-raising country, horse-raising history is long. Mongolian horse is the most abundant and widely distributed horse in China, which has the characteristics of strong tolerance, drought resistance, cold resistance and disease resistance, and is an extremely valuable gene bank in horse genetic resources. Therefore, the study on the blood glucose related genes of Mongolian horse in China can not only further understand the reasons of Mongolian horse endurance, but also provide a theoretical basis for revealing the genetic characteristics of Mongolian horse endurance. Moreover, it is of great significance to the improvement and cultivation of local horses. In this study, 6 Mongolian horses with similar genetic background and four-month exercise training were studied. Firstly, the physiological and biochemical indexes of Mongolian horse blood were measured by exercise training, and the basic data of blood physiological and biochemical indexes before and after training were analyzed by using SAS (the system for Windows 9.0) software. The results showed that blood glucose, creatine kinase, urea, hemoglobin, red blood cell, white blood cell, lymphocyte and platelet count showed significant changes before and after training. The blood glucose concentration before training was (4.77 鹵0.42) mmol / L, and that after training was (5.83 鹵0.31) mmol / L, which was significantly higher than that before training (P0.05). Secondly, high-throughput transcriptional sequencing of Mongolian horse skeletal muscle samples before and after training was carried out, and the differentially screened genes were enriched with go function on the term entry. The LPHN3 LGALS3.IFIH1 gene associated with blood sugar was screened as the target gene in this study. Real-time quantitative RT-PCR was used to detect the mRNA expression of LPHN3, LGALS3, IFIH1 gene in skeletal muscle and blood of Mongolian horse before and after training. The results showed that LPHN3LGALS3 IFIH1 gene was expressed in Mongolian horse blood and skeletal muscle before and after training. The expression of LPHN3 gene in the skeletal muscle of Mongolian horse after training was significantly higher than that before training (P0.01), and there was no significant difference between before and after training (P 鈮，

本文編號(hào)：2116711