本文選題：家兔 + 雌激素受體　； 參考：《中國(guó)畜牧雜志》2017年10期

【摘要】：家兔的繁殖性狀屬于低遺傳力性狀,基于傳統(tǒng)育種方法的遺傳改良很難取得顯著進(jìn)展。家兔雌激素受體1(ESR1)基因是影響繁殖性狀的重要候選基因,本研究利用PCR技術(shù)擴(kuò)增了德國(guó)巨型白兔ESR1基因的編碼區(qū)序列。結(jié)果表明:基因進(jìn)化樹與已知進(jìn)化樹一致,哺乳動(dòng)物的ESR1基因可能來自于同一個(gè)祖先基因;德國(guó)巨型白兔ESR1基因與豬的同源性最高(87.1%);預(yù)測(cè)的蛋白包括4個(gè)主要的功能區(qū)域A/B區(qū)(aa1-187)、C區(qū)(aa188-264)、D區(qū)(aa265-314)和E區(qū)(aa315-598);分析發(fā)現(xiàn)5個(gè)保守的磷酸化位點(diǎn),可進(jìn)一步作為與性狀關(guān)聯(lián)研究的候選位點(diǎn)。測(cè)定的德國(guó)巨型白兔ESR1編碼區(qū)序列將為基因功能突變和繁殖性狀的相關(guān)性研究提供理論基礎(chǔ)。
[Abstract]:The reproductive traits of rabbits are of low heritability, and it is difficult to make significant progress in genetic improvement based on traditional breeding methods. Rabbit estrogen receptor 1 (ESR1) gene is an important candidate gene affecting reproductive traits. In this study, the coding region of ESR1 gene of German giant white rabbit was amplified by polymerase chain reaction (PCR). The results showed that the gene evolution tree was consistent with the known evolutionary tree, and the ESR1 gene of mammal probably came from the same ancestor gene. The German giant white rabbit ESR1 gene had the highest homology with pigs (87.1%), and the predicted protein consisted of four major functional regions, A / B region (aa1-187), C region (aa188-264), D region (aa265-314) and E region (aa315-598), and five conserved phosphorylation sites were found. It can be further used as a candidate for the study of trait association. The sequence of ESR1 coding region of German giant white rabbit will provide a theoretical basis for the study of the correlation between gene functional mutation and reproductive traits.
【作者單位】： 四川省畜牧科學(xué)研究院動(dòng)物遺傳育種四川省重點(diǎn)實(shí)驗(yàn)室;
【基金】：四川省畜牧科學(xué)研究院基本科研業(yè)務(wù)費(fèi)專項(xiàng)資金(SASA2014A11) 公益性行業(yè)(農(nóng)業(yè))科研專項(xiàng)經(jīng)費(fèi)(201303143) 中國(guó)南方經(jīng)濟(jì)作物副產(chǎn)物飼料化利用技術(shù)研究與示范(201403049) 國(guó)家兔產(chǎn)業(yè)技術(shù)體系(CARS-44-B-4) 四川省育種攻關(guān)項(xiàng)目(2016NYZ0046) 四川省科技支撐計(jì)劃(2016NZ0002)
【分類號(hào)】：Q78;S829.1

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本文編號(hào)：2096623