本文選題：RIG- + 啟動(dòng)子��； 參考：《浙江大學(xué)學(xué)報(bào)(農(nóng)業(yè)與生命科學(xué)版)》2017年01期

【摘要】：RIG-1屬于細(xì)胞內(nèi)蛋白,與細(xì)胞增殖、分化和天然抗免疫功能密切相關(guān)。為研究RIG-1在鴨胚胎期免疫器官發(fā)育中的作用,本研究克隆得到鴨RIG-1啟動(dòng)子并做生物信息學(xué)分析,應(yīng)用實(shí)時(shí)熒光定量PCR技術(shù)檢測(cè)RIG-1基因以及預(yù)測(cè)得到的多個(gè)轉(zhuǎn)錄因子在鴨胚胎免疫器官發(fā)育過程中的表達(dá)模式。結(jié)果表明,擴(kuò)增得到鴨RIG-1基因啟動(dòng)子4 372bp。序列分析表明,該基因啟動(dòng)子存在典型的TATA-box、CAAT-box調(diào)控元件,有IRF-1、RXR、RAR、AP1、NF-κB、SP1、IL6及Pax-2等多個(gè)轉(zhuǎn)錄因子結(jié)合位點(diǎn)。此外,RIG-1啟動(dòng)子預(yù)測(cè)發(fā)現(xiàn)了一個(gè)CpG島,GC含量65.8%。定量結(jié)果發(fā)現(xiàn),RIG-1在鴨胚胎免疫器官中表達(dá)呈動(dòng)態(tài)性,具有不同的表達(dá)模式;并在法氏囊中的表達(dá)量高于脾臟和胸腺。聚類熱圖顯示,只有在法氏囊中RIG-1與IRF-1、RXR、AP1、NF-κB、IL6的mRNA表達(dá)量具有相似的表達(dá)模式,這可能是由于在鴨胚胎期法氏囊具有較為完整的結(jié)構(gòu)與功能,暗示它們可能為RIG-1的轉(zhuǎn)錄因子;而在法氏囊、脾臟和胸腺中,RIG-1與IRF-1、NF-κB基因都有較為相似的表達(dá)模式,在3個(gè)組織當(dāng)中均被聚類在一起,說明IRF-1和NF-κB可能參與調(diào)控RIG-1的表達(dá)。研究結(jié)果為探索鴨RIG-1基因轉(zhuǎn)錄調(diào)控、表達(dá),與在細(xì)胞增殖、分化、天然抗病毒免疫方面的功能提供了依據(jù)和方向。
[Abstract]:RIG-1 belongs to intracellular protein and is closely related to cell proliferation, differentiation and natural anti-immune function. In order to study the role of RIG-1 in the development of duck immune organs at embryonic stage, duck RIG-1 promoter was cloned and analyzed by bioinformatics. Real-time fluorescent quantitative PCR was used to detect the expression patterns of RIG-1 gene and several transcription factors in the development of duck embryonic immune organs. The results showed that the promoter of duck RIG-1 gene was 4372 BP. Sequence analysis showed that the promoter had a typical TATA-box CAAT-box regulatory element and several transcription factor binding sites, such as IRF-1, RXR, RARX, AP1, NF- 魏 B, SP1, Pax-2, and so on. In addition, a CpG insular GC content of 65.8% was found in the prediction of RIG-1 promoter. The quantitative results showed that the expression of RIG-1 was dynamic in the immune organs of duck embryos and had different expression patterns, and the expression level in bursa of Fabricius was higher than that in spleen and thymus. Clustering thermogram showed that only in bursa of Fabricius did RIG-1 and IRF-1RXRHAP1NF-kappa IL-6 have similar expression patterns, which may be due to the relatively complete structure and function of bursa of Fabricius at embryonic stage, suggesting that RIG-1 may be a transcription factor of RIG-1. In the bursa of Fabricius, spleen and thymus, the expression pattern of RIG-1 and IRF-1NF-1NF- 魏 B gene was similar, and it was clustered in three tissues, indicating that IRF-1 and NF- 魏 B might be involved in regulating the expression of RIG-1. The results provide the basis and direction for exploring the transcription regulation, expression and function of duck RIG-1 gene in cell proliferation, differentiation and natural antiviral immunity.
【作者單位】： 四川農(nóng)業(yè)大學(xué)動(dòng)物遺傳育種研究所;
【基金】：國家自然科學(xué)基金(31301964) 四川省科技支撐應(yīng)用基礎(chǔ)項(xiàng)目(2015JY0110)
【分類號(hào)】：S834

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