發(fā)布時間：2018-06-18 20:08

  本文選題：奶牛 + 生物素��； 參考：《河南農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：本研究結(jié)合現(xiàn)在奶牛養(yǎng)殖業(yè)中面臨的繁殖性能低下的問題,在初產(chǎn)母牛日糧中添加不同濃度的生物素,通過對發(fā)情率、受配率、受胎率、分娩率、產(chǎn)犢間隔、產(chǎn)后發(fā)情時間等繁殖性能指標(biāo)進行統(tǒng)計分析后,篩選出最佳的生物素添加劑量。并研究其對卵巢機能的影響。此外,分別在試驗牛的不同發(fā)情周期階段,添加生物素后主要生殖激素的變化規(guī)律,從內(nèi)分泌機制上闡明生物素對奶牛繁殖性能的作用機制。并研究了不同劑量生物素對奶牛卵巢卵泡發(fā)育的影響。研究結(jié)果如下:1.不同劑量生物素對奶牛的發(fā)情受胎效果試驗研究了在奶�；A(chǔ)日糧基礎(chǔ)上不同劑量生物素對奶牛發(fā)情受胎的影響。結(jié)果表明:試驗結(jié)果表明:添加20 mg.d-1(69.44%)、30 mg.d-1(70.43%)與40 mg.d-1(68.80%)生物素組的母牛發(fā)情率極顯著(P0.01)高于10 mg.d-1組(62.86%)與對照組(55.24%),且10 mg.d-1組(62.86%)顯著(P0.05)高于對照組(55.24%),而20 mg.d-1、30 mg.d-1與40 mg.d-1生物素組間差異不顯著(P0.05)。添加20 mg.d-1(90.67%)、30 mg.d-1(91.36%)與40 mg.d-1(89.53%)生物素組的母�？偸芴ヂ蕵O顯著(P0.01)高于10 mg.d-1組(84.85%)與對照組(84.48%),10 mg.d-1組的總受胎率雖高于對照組,但兩者差異不顯著(P0.05),且30 mg.d-1組的受胎率略高于20 mg.d-1與40 mg.d-1組,但3組間差異也不顯著(P0.05)。研究結(jié)果說明日糧中添加20 mg·d-1、30 mg·d-1與40 mg·d-1生物素對奶牛的發(fā)情與受胎效果要明顯好于其他兩組(0 mg·d-1與10 mg·d-1)。建議添加量以20 mg.d-1~30 mg.d-1生物素為宜。2.不同劑量生物素對奶牛產(chǎn)犢率的影響研究結(jié)果顯示:20 mg.d-1組(82.67%)、30 mg.d-1組(86.42%)與40 mg.d-1組(83.72%)的母牛產(chǎn)犢率極顯著(P0.01)高于對照組(70.69%),且顯著(P0.05)高于10 mg.d-1組(75.76%),10 mg.d-1組的產(chǎn)犢率顯著(P0.05)高于對照組,30 mg.d-1組的母牛產(chǎn)犢率略高于20 mg.d-1與40 mg.d-1組,但3組間差異不顯著(P0.05)。研究結(jié)果說明不同劑量生物素對母牛產(chǎn)犢率有明顯影響,以30 mg·d-1劑量為最高。3.不同劑量生物素對奶牛產(chǎn)犢指數(shù)的影響20 mg.d-1組(378.23±12.82)、30 mg.d-1組(375.71±10.95)與40 mg.d-1組(379.17±9.81)的母牛產(chǎn)犢指數(shù)極顯著(P0.01)低于對照組(396.34±14.50),且顯著(P0.05)低于10mg.d-1組(385.80±10.76),10 mg.d-1組(385.80±10.76)的產(chǎn)犢指數(shù)顯著(P0.05)低于對照組,30 mg.d-1組(375.71±10.95)組的母牛產(chǎn)犢指數(shù)略低于20 mg.d-1組(378.23±12.82)與40 mg.d-1組(379.17±9.81),但3組間差異不顯著(P0.05)。研究結(jié)果表明添加生物素可以明顯縮短母牛的產(chǎn)犢間隔(產(chǎn)犢指數(shù))。不同劑量生物素對母牛產(chǎn)犢指數(shù)有顯著影響,以30 mg·d-1劑量為最佳。4.不同劑量生物素對奶牛產(chǎn)后發(fā)情的影響20mg.d-1組(78.67%)、30mg.d-1組(80.25%)與40mg.d-1組(79.07%)的母牛產(chǎn)后30d發(fā)情率極顯著(p0.01)高于對照組(51.72%),且顯著(p0.05)高于10mg.d-1組(69.70%),10mg.d-1組的母牛產(chǎn)后30d發(fā)情率顯著(p0.05)高于對照組,但20mg.d-1組、30mg.d-1組與40mg.d-1組間差異不顯著(p0.05)。20mg.d-1組(63.37±10.96)、30mg.d-1組(61.97±8.91)與40mg.d-1組(62.65±9.08)的母牛從分娩至第一次配種平均天數(shù)顯著(p0.05)低于10mg.d-1組(69.76±11.23)與對照組(72.19±14.79),10mg.d-1組的母牛從分娩至第一次配種平均天數(shù)雖低于對照組,但兩者差異不顯著(p0.05),且20mg.d-1組、30mg.d-1組與40mg.d-1組間差異不顯著(p0.05)。本試驗結(jié)果顯示生物素可以明顯提高母牛產(chǎn)后發(fā)情率,并縮短母牛產(chǎn)后發(fā)情與配種時間。不同劑量生物素對母牛產(chǎn)后發(fā)情率與母牛產(chǎn)后發(fā)情和配種時間有顯著影響,以30mg·d-1劑量為最佳。5.不同劑量生物素對奶牛發(fā)情周期不同時間雌二醇水平的影響結(jié)果表明:母牛在發(fā)情周期3d,20mg.d-1組(5.91±0.97pg/ml)、30mg.d-1組(5.95±0.83pg/ml)與40mg.d-1組(5.98±1.17pg/ml)組的母牛血漿雌二醇含量顯著(p0.05)高于10mg.d-1組(5.01±0.73pg/ml)與對照組(4.93±0.59pg/ml),10mg.d-1組的母牛血漿雌二醇含量高于對照組,但兩者差異不顯著(p0.05),且20mg.d-1組、30mg.d-1組與40mg.d-1組間差異不顯著(p0.05)。母牛在發(fā)情周期20d(發(fā)情前1d),20mg.d-1組(12.53±2.01pg/ml)、30mg.d-1組(12.91±1.51pg/ml)組與40mg.d-1組(12.57±1.13pg/ml)組的母牛血漿雌二醇含量極顯著(p0.01)高于對照組(8.92±0.57pg/ml),且顯著(p0.05)高于10mg.d-1組(10.55±1.72pg/ml),10mg.d-1組的母牛血漿雌二醇含量顯著(p0.05)高于對照組,且20mg.d-1組、30mg.d-1組與40mg.d-1組間差異不顯著(p0.05)。研究結(jié)果說明日糧中添加20mg·d-1、30mg·d-1與40mg·d-1生物素可以明顯提高血漿雌二醇的基礎(chǔ)水平與峰值。6.不同劑量生物素對奶牛發(fā)情周期不同時間孕酮水平的影響母牛在發(fā)情周期0d(發(fā)情當(dāng)天),20mg.d-1組(0.33±0.04ng/ml)、30mg.d-1組(0.35±0.06ng/ml)組與40mg.d-1組(0.34±0.07ng/ml)的母牛血漿孕酮含量極顯著(p0.01)高于對照組(0.27±0.04ng/ml),且顯著(p0.05)高于10mg.d-1組(0.27±0.03ng/ml)組,10mg.d-1組的母牛血漿孕酮含量顯著(p0.05)高于對照組,且20mg.d-1組、30mg.d-1組與40mg.d-1組間差異不顯著(p0.05)。母牛在發(fā)情周期14d,20mg.d-1組(7.43±0.81ng/ml)、30mg.d-1組(7.51±0.74ng/ml)組與40mg.d-1組(7.52±0.73ng/ml)組的母牛血漿孕酮含量極顯著(p0.01)高于對照組(6.57±0.35ng/ml),且顯著(p0.05)高于10mg.d-1組(6.91±0.79ng/ml)組,10mg.d-1組組的母牛血漿孕酮含量顯著(p0.05)高于對照組,且20mg.d-1組、30mg.d-1組與40mg.d-1組間差異不顯著(p0.05)。研究結(jié)果說明日糧中添加20 mg·d-1、30 mg·d-1與40 mg·d-1生物素可以明顯提高血漿孕酮的基礎(chǔ)水平與峰值。7.不同劑量生物素對奶牛卵巢卵泡發(fā)育的影響20 mg.d-1組(7.69±1.98)、30 mg.d-1組(7.84±1.35)組與40 mg.d-1組(7.82±3.54)的平均中卵泡數(shù)極顯著(P0.01)高于對照組(3.45±1.77),且顯著(P0.05)高于10 mg.d-1組(5.38±2.01)組,10 mg.d-1組組的平均中卵泡數(shù)(P0.05)高于對照組,且20 mg.d-1組、30 mg.d-1組與40 mg.d-1組間差異不顯著(P0.05)。20 mg.d-1組(13.13±5.84)、30mg.d-1組(13.36±3.98)與40 mg.d-1組(12.94±6.87)的平均小卵泡數(shù)顯著(P0.05)低于10 mg.d-1組(16.67±7.32)組與對照組(17.97±7.91),對照組的平均小卵泡數(shù)雖高于10mg.d-1組組,但兩者差異不顯著(P0.05),且20 mg.d-1組、30 mg.d-1組與40 mg.d-1組間差異不顯著(P0.05)。5組間的平均卵泡總數(shù)差異也不顯著(P0.05)。研究結(jié)果說明日糧中添加20 mg·d-1、30 mg·d-1與40 mg·d-1生物素對奶牛卵巢上的中卵泡數(shù)量明顯高于其他2組,由于中卵泡中排出的卵母細(xì)胞受胎率高于小卵泡與大卵泡。因此,為了提高奶牛繁殖力,建議以添加20 mg·d-1~30 mg·d-1生物素為宜。
[Abstract]:This study combined with the problem of low reproductive performance in the dairy cattle breeding industry, adding different concentrations of biotin in the primary cow's diet, and screening out the best biotin dosage after analyzing the reproductive performance indexes, such as the estrous rate, the rate of pregnancy, the rate of birth, the birth rate, the interval of calving, the time of postpartum estrus and so on. The effects on ovarian function were studied. In addition, the changes in the main reproductive hormones were added to the different estrous cycle stages of the test cattle, and the mechanism of the effects of biotin on the reproductive performance of cows was elucidated from the endocrine mechanism. The effects of different doses of biotin on the ovarian follicle development of dairy cows were studied. The effects of different doses of biotin on the estrus of cows were studied in 1. different doses. The effects of different doses of biotin on cow's estrus on dairy cows were studied. The results showed that the experimental results showed that the oestrus rate of the cows with 20 mg.d-1 (69.44%), 30 mg.d-1 (70.43%) and 40 mg.d-1 (68.80%) was significantly higher than that of the cows (P0.01). The 10 mg.d-1 group (62.86%) and the control group (55.24%), and the 10 mg.d-1 group (62.86%) were significantly higher than the control group (55.24%), while the difference between the 20 mg.d-1,30 mg.d-1 and the 40 mg.d-1 biotin group was not significant (P0.05). The addition of 20 mg.d-1 (90.67%), 30 mg.d-1 (91.36%) and 40 mg.d-1 (89.53%) biotin group was very significant (P0.01) higher than that of the mg.d-1. Group (84.85%) and control group (84.48%), the total pregnancy rate in the 10 mg.d-1 group was higher than the control group, but the difference was not significant (P0.05), and the 30 mg.d-1 group was slightly higher than the 20 mg.d-1 and 40 mg.d-1 groups, but the difference between the 3 groups was not significant (P0.05). The results showed that the diet added 20 mg. D-1,30 mg D-1 and 40 mg D-1 biotin to the cows. The effect of emotion and pregnancy was better than that of the other two groups (0 mg / D-1 and 10 mg D-1). The results showed that the effects of 20 mg.d-1~30 mg.d-1 biotin on the calving rate of dairy cows in different doses of suitable.2. showed that the calving rate of 20 mg.d-1 group (82.67%), 30 mg.d-1 group (86.42%) and 40 mg.d-1 group (83.72%) was significantly higher than that of the pair. Group (70.69%), and significant (P0.05) higher than the 10 mg.d-1 group (75.76%), 10 mg.d-1 group calving rate significantly higher than the control group, 30 mg.d-1 group cow calving rate is slightly higher than the 20 mg.d-1 and 40 mg.d-1 groups, but the 3 groups have no significant difference (P0.05). The results show that different doses of biotin has a significant impact on cow calving rate, with 30 mg D-1 agent. The effect of different doses of.3. on the calving index of dairy cows was 20 mg.d-1 (378.23 + 12.82), 30 mg.d-1 group (375.71 + 10.95) and 40 mg.d-1 group (379.17 + 9.81) of cow calving index (P0.01) was lower than that of the control group (396.34 + 14.50), and significantly lower than that of 10mg.d-1 group (385.80 + 10.76) and 10 mg.d-1 group. The calving index (P0.05) was lower than that of the control group. The calving index of the cows in the 30 mg.d-1 group (375.71 + 10.95) was slightly lower than the 20 mg.d-1 group (378.23 + 12.82) and 40 mg.d-1 group (379.17 + 9.81), but the difference between the 3 groups was not significant (P0.05). The results showed that the addition of biotin could significantly shorten the calving interval (calving index) of the cows. The calving index had a significant influence on cow calving index. The effect of 30 mg / D-1 dose on the best.4. different doses of biotin on the postpartum estrus was in the group 20mg.d-1 (78.67%). The 30d oestrus rate of the 30mg.d-1 group (80.25%) and the 40mg.d-1 group (79.07%) was significantly higher than the control group (51.72%), and the significant (P0.05) was higher than the 10mg.d-1 group (69.70%), 10mg.d-1. The rate of 30d estrus was significantly higher in the group of cows (P0.05) than in the control group, but in group 20mg.d-1, there was no significant difference between group 30mg.d-1 and 40mg.d-1 group (P0.05).20mg.d-1 group (63.37 + 10.96), 30mg.d-1 group (61.97 + 8.91) and 40mg.d-1 group (62.65 + 9.08) from childbirth to first mating average days (P0.05) was lower than that of 10mg.d-1 group (69.76 + 11.23). In the control group (72.19 + 14.79), the average days in the 10mg.d-1 group were lower than the control group, but the difference between the two groups was not significant (P0.05), and the difference between the group 30mg.d-1 and the 40mg.d-1 group was not significant (P0.05). The results of this test showed that biotin could obviously improve the estrous rate of the cows and shorten the estrus postpartum. The effects of different doses of biotin on the postpartum estrus and mating time of cows were significant. The effect of 30mg D-1 dose on the estradiol levels of the estrous cycle at different doses of.5. at different doses of.5. showed that the cows were in the oestrus cycle 3D, 20mg.d-1 group (5.91 + 0.97pg/ml), 30mg.d-1 group (30mg.d-1 group). 5.95 + 0.83pg/ml) and 40mg.d-1 group (5.98 + 1.17pg/ml) group of cows plasma estradiol content was significantly higher (P0.05) than the 10mg.d-1 group (5.01 + 0.73pg/ml) and the control group (4.93 + 0.59pg/ml), 10mg.d-1 group cow plasma estradiol content is higher than the control group, but the difference is not significant (P0.05), and 20mg.d-1, 30mg.d-1 group and 40mg.d-1 groups are not different. Significant (P0.05). Cows in the estrous cycle 20d (pre estrus 1D), 20mg.d-1 group (12.53 + 2.01pg/ml), 30mg.d-1 group (12.91 + 1.51pg/ml) and 40mg.d-1 group (12.57 + 1.13pg/ml) group of cows plasma estradiol content is very significant (P0.01) higher than the control group (8.92 + 0.57pg/ ml), and significantly higher than the group (10.55 +), the mother of the mother The content of estradiol in bovine plasma (P0.05) was significantly higher than that in the control group, and in group 20mg.d-1, there was no significant difference between group 30mg.d-1 and 40mg.d-1 group (P0.05). The results showed that the addition of 20mg, d-1,30mg, D-1 and 40mg D-1 biotin in the diet could significantly improve the basal level of plasma estradiol and the peak.6. dose of biotin on the estrous cycle of dairy cows. The level of progesterone at the same time affects cows in the estrous cycle 0d (estrus day), group 20mg.d-1 (0.33 + 0.04ng/ml), 30mg.d-1 group (0.35 + 0.06ng/ml) and 40mg.d-1 group (0.34 + 0.07ng/ml) of maternal plasma progesterone content is very significant (P0.01) higher than the control group (0.27 + 0.04ng/ml), and significant (P0.05) higher than the 10mg.d-1 group (0.27 + 0.03ng/ml) group, The plasma progesterone content of the cow plasma was significantly higher (P0.05) than that in the control group, and there was no significant difference between the 20mg.d-1 group and the 30mg.d-1 group (P0.05). The cows were in the estrous cycle 14d, the 20mg.d-1 group (7.43 + 0.81ng/ml), the 30mg.d-1 group (7.51 + 0.74ng/ml) and the 40mg.d-1 group (7.52 + 0.73ng/ml) were significantly higher than the control group. Group (6.57 + 0.35ng/ml), and significant (P0.05) higher than group 10mg.d-1 (6.91 + 0.79ng/ml), 10mg.d-1 group of cows plasma progesterone content (P0.05) higher than the control group, and 20mg.d-1 group, 30mg.d-1 group and 40mg.d-1 group difference is not significant (P0.05). The influence of the basic level of plasma progesterone and peak.7. on the ovarian follicle development of dairy cows was 20 mg.d-1 (7.69 + 1.98). The average number of follicles in the 30 mg.d-1 group (7.84 + 1.35) and 40 mg.d-1 group (7.82 + 3.54) was significantly higher than that of the control group (3.45 + 1.77), and the significant (P0.05) was higher than that of the 10 mg.d-1 group (5.38 + 2.01). The average median follicle number (P0.05) in group mg.d-1 was higher than that in the control group, and the difference between the 20 mg.d-1 group and the 30 mg.d-1 group and the 40 mg.d-1 group was not significant (P0.05).20 mg.d-1 group (13.13 + 5.84), the average number of small follicles in 30mg.d-1 group (13.36 + 3.98) and 40 mg.d-1 group (P0.05) was lower than that of 10 mg.d-1 group (16.67 + 7.32) and control group (16.67 + 5.84), The average number of small follicles in the control group was higher than the 10mg.d-1 group, but the difference was not significant (P0.05), and the difference between the 20 mg.d-1 group, the 30 mg.d-1 group and the 40 mg.d-1 group was not significant (P0.05) the difference of the average follicle number between the.5 group was not significant (P0.05). The results showed that the diet added 20 mg d-1,30 mg, D-1 and 40 extracellular biotin to cow eggs. The number of middle follicles on the nests is obviously higher than that of the other 2 groups, because the rate of oocyte in the middle follicles is higher than that of the small follicles and large follicles. Therefore, it is advisable to add 20 mg. Mg. D-1 biotin in order to improve the fecundity of the cows.
【學(xué)位授予單位】：河南農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S823

【相似文獻】

相關(guān)期刊論文 前10條

1 李俊杰,桑潤滋,田樹軍;熱應(yīng)熱對奶牛繁殖性能的影響[J];中國奶牛;2001年02期

2 韋公遠;提高奶牛繁殖性能的若干途徑[J];中國奶牛;2002年06期

3 韋公遠;提高奶牛繁殖性能的若干途徑[J];黑龍江動物繁殖;2002年03期

4 施振旦,邵西兵,梅承,林輝新,樊潤財;提高奶牛繁殖性能的技術(shù)發(fā)展現(xiàn)狀[J];中國奶牛;2004年02期

5 李章鎖;;提高奶牛繁殖性能的新技術(shù)(一)[J];農(nóng)村百事通;2006年14期

6 馬群山;付云超;李善文;薛琳琳;;微量元素對奶牛繁殖性能的影響[J];養(yǎng)殖技術(shù)顧問;2006年12期

7 叢智慧;譚支良;周傳社;湯少勛;孫志洪;;影響奶牛繁殖性能的營養(yǎng)因素及其調(diào)控[J];家畜生態(tài)學(xué)報;2006年06期

8 魏慶軍;;營養(yǎng)對奶牛繁殖性能的影響[J];北方牧業(yè);2007年04期

9 付云超;李善文;薛琳琳;馬群山;;微量元素對奶牛繁殖性能的影響[J];河南畜牧獸醫(yī)(綜合版);2007年04期

10 吳晨晨;夏成;張靜;高峰;胡恩偉;田立志;;散欄式集約化牛場奶牛繁殖性能狀況的調(diào)查[J];黑龍江畜牧獸醫(yī);2007年10期

相關(guān)會議論文 前5條

1 吳俊靜;楊利國;宴邦富;劉曉華;錢運國;金爾光;滕茂平;李開橋;熊敏;程橙;張淑君;;奶牛繁殖性能的分析研究[A];中國奶業(yè)協(xié)會第24次繁殖學(xué)術(shù)年會暨國家奶牛/肉牛產(chǎn)業(yè)技術(shù)體系第一屆全國牛病防治學(xué)術(shù)研討會論文集[C];2009年

2 王治國;王傳蓉;;營養(yǎng)因素對奶牛繁殖性能的影響[A];第三屆中國奶業(yè)大會論文集(上冊)[C];2012年

3 王學(xué)君;劉偉;苗霆;王錦;;能量對奶牛繁殖性能的影響與調(diào)控技術(shù)[A];中國奶業(yè)協(xié)會年會論文集2008（上冊）[C];2008年

4 許美解;何華西;;中草藥飼料添加劑提高奶牛繁殖性能的研究[A];中國奶業(yè)協(xié)會第24次繁殖學(xué)術(shù)年會暨國家奶牛/肉牛產(chǎn)業(yè)技術(shù)體系第一屆全國牛病防治學(xué)術(shù)研討會論文集[C];2009年

5 黃友梅;;如何提高奶牛繁殖性能[A];中國奶業(yè)協(xié)會第24次繁殖學(xué)術(shù)年會暨國家奶牛/肉牛產(chǎn)業(yè)技術(shù)體系第一屆全國牛病防治學(xué)術(shù)研討會論文集[C];2009年

相關(guān)重要報紙文章 前2條

1 葉日松;營養(yǎng)對奶牛繁殖性能的影響[N];中國畜牧報;2003年

2 魏之福;制約高產(chǎn)奶牛繁殖性能的瓶頸[N];中國畜牧獸醫(yī)報;2007年

相關(guān)碩士學(xué)位論文 前5條

1 劉坤;大型牛場奶牛繁殖性能調(diào)查及提高奶牛受胎率的研究[D];南京農(nóng)業(yè)大學(xué);2012年

2 董宏偉;大豆油脂對奶牛繁殖性能和泌乳性能的影響[D];吉林農(nóng)業(yè)大學(xué);2007年

3 李紅宇;日糧蛋白質(zhì)水平對奶牛繁殖性能的影響[D];黑龍江八一農(nóng)墾大學(xué);2010年

4 李曉飛;生物素對奶牛繁殖性能的影響及其內(nèi)分泌機制研究[D];河南農(nóng)業(yè)大學(xué);2015年

5 朱靜;奶牛場精準(zhǔn)管理與資源匹配研究[D];甘肅農(nóng)業(yè)大學(xué);2010年

，

本文編號：2036711