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  本文選題：耐熱腸毒素 + 重組大腸桿菌�。� 參考：《畜牧獸醫(yī)學報》2017年09期

【摘要】：旨在研究表達Ⅰ型耐熱腸毒素(STa)的重組大腸桿菌對7日齡仔豬腸道吸收、屏障、轉運及抗氧化功能的影響。選取24頭7日齡仔豬,分成4個處理組,分別為對照組(基礎日糧)、STa組(基礎日糧+2×109 CFU重組菌LMG194-STa)、LMG194組(基礎日糧+2×109 CFU大腸桿菌LMG194)和K88組(基礎日糧+2×109 CFU大腸桿菌K88)。使用人工乳飼養(yǎng),第5天進行攻毒,第7天屠宰取樣。測量回腸免疫相關基因IFN-γ、IL-4與VNN1及回腸黏膜損傷基因Villin與MMP3的相對轉錄量,空腸黏膜上皮連接蛋白Occludin與Claudin-1相對表達量,以及十二指腸、回腸氧化相關酶谷胱甘肽過氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、髓過氧化物酶(MPO)的活力與氧化相關產物丙二醛(MDA)的含量。試驗結果表明,與對照組相比,STa組顯著降低了回腸IFN-γ和IL-4基因相對轉錄量,VNN1相對轉錄量顯著上調(P0.05);STa組顯著降低了基因Villin與MMP3相對轉錄量(P0.05)及蛋白Occludin與Claudin-1相對表達量(P0.05);同時,STa組十二指腸、回腸GSH-Px和回腸MPO活力顯著降低(P0.05);STa組十二指腸丙二醛(MDA)含量顯著升高(P0.05)。結果顯示,表達Ⅰ型耐熱腸毒素的重組大腸桿菌可導致7日齡仔豬腸道免疫、屏障及抗氧化功能下降。
[Abstract]:The aim of this study was to investigate the effects of recombinant Escherichia coli expressing heat resistant enterotoxin type I (STA) on intestinal absorption, barrier, transport and antioxidant function in 7-day-old piglets. 24 7-day-old piglets were divided into four groups: control group (basal diet 2 脳 109 CFU recombinant bacteria LMG194) and K88 group (basal diet 2 脳 109 CFU Escherichia coli LMG194) and K88 group (basal diet 2 脳 109 CFU Escherichia coli K88). Artificial milk was used to feed, 5 days to attack the drug, 7 days slaughtering sampling. The relative transcription of IFN- 緯 -IL-4 and VNN1, the ileal mucosal injury gene Villin and MMP3, the relative expression of jejunal mucosal junction protein Occludin and Claudin-1, and duodenum were measured. The activities of glutathione peroxidase (Glutathione peroxidase), superoxide dismutase (SOD) and myeloperoxidase (MPO) and the content of malondialdehyde (MDA) in ileum. The results show that Compared with the control group, the relative transcription of IFN- 緯 and IL-4 genes in the ileum and the relative transcription of VNN1 were significantly increased in the STA group compared with the control group. In the STA group, the relative transcription of Villin and MMP3 decreased significantly, and the relative expression of protein Occludin and Claudin-1 decreased P0.05in the duodenum of STa group. The activities of GSH-Px and MPO in ileum significantly decreased the content of malondialdehyde (MDAs) in duodenum of STA group. The results showed that recombinant Escherichia coli expressing heat-resistant enterotoxin type I could decrease intestinal immunity, barrier and antioxidant function in 7-day-old piglets.
【作者單位】： 武漢輕工大學動物營養(yǎng)與飼料科學湖北省重點實驗室;中國水產科學研究院長江水產研究所;
【基金】：國家自然科學基金(31302089) 湖北省自然科學基金(2012FFBO4804)
【分類號】：S858.28

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