本文選題：IUGR + 胎兒肝臟�。� 參考：《內(nèi)蒙古農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：本實驗主要研究蒙古綿羊妊娠后期營養(yǎng)限飼引起胎兒宮內(nèi)生長限制對胎兒肝臟生長發(fā)育及其生理功能的影響。選擇體況中等、2-3胎次的健康蒙古綿羊24只,采用同期發(fā)情技術(shù)使其同一時期受孕,到妊娠期90d時,選出6只母羊,開始屠宰實驗,其余按體重任意分配為3組,每組6只并采取不同的能量飼養(yǎng)水平：CG組(自由采食)；RG2組(0.33 MJME·kgw-0.75·d-1); RG1組(0.175MJME·kgw-0.75·d-1),能量水平依次降低,至妊娠140d時對全部實驗組母羊進(jìn)行屠宰。實驗研究結(jié)果表明,在本實驗條件下：(1)IURG對胎兒肝臟發(fā)育的影響：胎兒肝臟重、肝臟生長速率顯著降低；在胎兒肝臟組織中,RG1組DNA、蛋白濃度,總DNA含量及蛋白質(zhì)/DNA均顯著低于CG組(P0.01);RG1組胎兒肝細(xì)胞數(shù)量、肝細(xì)胞直徑、枯否氏細(xì)胞數(shù)量均有減少,內(nèi)皮細(xì)胞數(shù)量增多；限飼組胎兒肝臟組分均顯著降低。(2)IURG對胎兒肝臟代謝功能的影響：RG1組胎兒肝臟組織中的TC、TG、GLU、LD含量(P0.01)以及PA含量(P0.05)和LPL、HL活性(P0.05)均顯著低于CG組；胎兒血中GLU濃度有升高趨勢,LD、BHBA濃度(P0.01)以及NEFA、TC、PA濃度(P0.05)均顯著高于CG組,TG含量(P0.05)顯著低于CG組,而TP、ALB、GLB濃度(P0.05)有降低趨勢。(3)IURG對胎兒肝臟免疫功能的影響：RG1組胎兒肝臟中的補體C3含量(P0.01)顯著高于CG組,補體C4含量(P0.05)有逐漸升高趨勢。(4)IURG對胎兒肝臟造血功能的影響：胎兒肝臟中RG1組EPO濃度、胎兒血液中RG1組RBC、HCT、HGB均顯著低于CG組(P0.01),PLT、WBC較CG組有降低趨勢(P0.05)。(5)IURG對胎兒肝臟急性期蛋白含量的影響：RG1組胎兒肝臟中IL-1β、IL-2、 IFN-γ濃度及HPT、CRP、CP含量均顯著低于CG組(P0.05),而TNF-a濃度、IL-4、SAA含量較CG組降低(P0.05)；胎兒血液中RGl組SAA(P＜0.05)、 CRP(P0.05)、CP(P0.01)濃度高于CG組,而HPT濃度低于CG組(P0.05)。
[Abstract]:The effect of fetal intrauterine growth restriction on fetal liver growth and its physiological function was studied. Twenty-four healthy Mongolian sheep with 2-3 parity were selected to conceive in the same period by synchronizing estrus technique. At 90 days of gestation, 6 ewes were selected and slaughtered. The rest were randomly divided into 3 groups according to their body weight. Six rats in each group were fed with different energy levels (0.33MJME kgw-0.75 d-1) in RG2 group and 0.175 MJME kgw-0.75 d-1 in RG1 group. The energy level was decreased in turn and slaughtered on day 140 of gestation. The experimental results showed that under the experimental conditions, the effects of 1: 1 + IURG on fetal liver development were as follows: fetal liver weight and liver growth rate decreased significantly, and DNA, protein concentration of RG1 group in fetal liver tissue decreased significantly. The total DNA content and protein / DNA content were significantly lower than those in CG group (P 0.01). The number of fetal hepatocytes, hepatocyte diameter and Kupffer's cells decreased, and the number of endothelial cells increased. The effect of Fetal liver components of Fetal liver in Fetal liver restriction Group on the Metabolic function of Fetal liver; the contents of TCG-GUULD, PA and P0.05) and the activity of LPLHL-P0.05) were significantly lower in the Fetal liver tissue of the 10 ~ (-1) RG1 group than in the CG group (P _ (0.05), and the difference was significant (P _ (0.05) between the control group and the control group (P _ (0.05). The concentration of GLU in fetal blood was significantly higher than that in CG group (P 0.01) and the concentration of Nefa TCU PA was significantly higher than that in CG group (P 0.05), which was significantly lower than that in CG group. The effect of IURG on the immune function of fetal liver was significantly higher than that in CG group. The effect of IURG on fetal liver hematopoiesis: the concentration of EPO in the RG1 group of fetal liver. The effect of RG1 group on the protein content of fetal liver in acute phase is significantly lower than that of CG group (P0.01P0.01P0.01P0.01P0. 01). Effect of IURG on the protein content of fetal liver in acute phase; the concentration of IL-1 尾 -IL-2, IFN- 緯 and HPT-CRPPCP in the fetal liver of the group of 10: RG1 are significantly lower than those of the CG group, while the concentration of IL-4a is significantly lower than that of the CG group (P 0.05), while the concentration of IL-4 and SAA of TNF-a is significantly lower than that of the CG group (P < 0.05), while the concentration of IL-4 and SAA is significantly lower than that of the group of CG. Compared with CG group, the quantity of P0. 05 was lower than that of CG group. The concentration of SAA (P < 0.05), CPP (0.01) in RGl group was higher than that in CG group, but HPT was lower than that in CG group (P 0.05).
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S826

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