本文選題：水貂 + 阿留申病�。� 參考：《東北農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：水貂(Mink)是哺乳綱鼬科鼬屬的肉食性動(dòng)物,主要用以皮毛生產(chǎn),是世界三大毛皮商品之一,被譽(yù)為“裘皮之王”。目前市場上除了黑褐色之外,還出現(xiàn)了各種色澤艷麗的彩色水貂。而水貂除毛皮之外,還可制作許多副產(chǎn)品�？芍^是由內(nèi)而外都具有極高的經(jīng)濟(jì)價(jià)值。水貂阿留申病是養(yǎng)貂產(chǎn)業(yè)公認(rèn)的三大疫病之一。主要侵害水貂的免疫系統(tǒng),引發(fā)動(dòng)脈血管炎、腎小球腎炎等疾病。患病水貂消瘦、貧血、衰弱,皮毛質(zhì)量差。一般對患病母貂造成空懷、流產(chǎn)、弱胎,而公貂精子活力和配種能力降低,造成嚴(yán)重的經(jīng)濟(jì)損失。研究表明,阿留申病因其特殊的發(fā)病機(jī)理,目前還沒有安全有效的治療方法。所以要想做好水貂阿留申病的控制工作,最大程度的降低該病對水貂產(chǎn)業(yè)造成的損失,最有效的手段就是在養(yǎng)殖過程中制定嚴(yán)格的疾病檢測制度,及時(shí)發(fā)現(xiàn)并淘汰病貂。因此,國內(nèi)外學(xué)者探究出多種水貂阿留申病的檢測手段,在水貂的生產(chǎn)養(yǎng)殖中得以廣泛應(yīng)用。糞便DNA提取方法是非損傷性取樣中的一種,該方法在取樣過程中避免了對動(dòng)物個(gè)體的干擾和損傷,且檢測樣本量大、成本低廉,在動(dòng)物分子生物學(xué)研究中具有很高的實(shí)際意義和應(yīng)用價(jià)值。水貂作為一種名貴的毛皮經(jīng)濟(jì)動(dòng)物,在對其進(jìn)行疾病檢測過程中,應(yīng)注意保護(hù)它的經(jīng)濟(jì)價(jià)值,盡量降低對其造成影響。在水貂阿留申病的檢測過程中,利用糞便DNA提取的檢測方法,既可以避免對水貂個(gè)體造成影響和損傷,又能夠準(zhǔn)確的測定其阿留申病的感染情況,具有很高的使用操作價(jià)值。因此,本研究利用水貂糞便DNA提取技術(shù),來檢測水貂體內(nèi)阿留申病毒,并以此為基礎(chǔ),探討阿留申病對水貂產(chǎn)仔數(shù)的影響。主要試驗(yàn)內(nèi)容結(jié)果如下:(1)利用Ezup柱式病毒DNA抽提試劑盒,成功的從患病水貂糞便中,抽提出阿留申病毒DNA。說明該種方法可以用于水貂阿留申病的檢測。(2)從產(chǎn)仔數(shù)高、低兩組糞樣中抽提的阿留申病毒DNA經(jīng)PCR擴(kuò)增后,電泳結(jié)果顯示,低產(chǎn)仔數(shù)組帶毒率高于高產(chǎn)仔數(shù)組。說明阿留申病對水貂產(chǎn)仔數(shù)有影響,引起水貂產(chǎn)仔數(shù)降低。綜上所述,本研究證實(shí),病毒DNA抽提試劑盒可以提取水貂糞便中阿留申病毒DNA,該方法可以用以水貂阿留申病的檢測;水貂阿留申病影響水貂產(chǎn)仔個(gè)數(shù),造成患病水貂產(chǎn)仔數(shù)下降。
[Abstract]:Mink (Mink) is a carnivorous animal of the weasel genus of the weasel family. It is mainly used for fur production. It is one of the world's three largest fur goods. It is known as the "king of fur". In addition to the black brown market, there are many colorful colored mink in the market. Sable Aleutian disease is one of the three major diseases recognized by mink industry. It mainly infringes the immune system of mink, causes arterial vasculitis, glomerulonephritis and other diseases. The sable is emaciated, anemic, weak, and poor in fur quality. The study shows that there is no safe and effective treatment for Aleutian disease, so it is necessary to make the control of Aleutian disease and reduce the loss of the mink industry to the greatest extent. The most effective means is to make it in the breeding process. Therefore, domestic and foreign scholars have explored a variety of mink Aleutian disease detection methods, and have been widely used in the production of mink. The method of DNA extraction is one of non damage sampling. This method avoids the interference and damage to animal individuals during the sampling process. It has high practical significance and application value in the study of animal molecular biology. As a valuable animal, mink should protect its economic value and reduce its impact on it in the process of disease detection. In the detection process of Aleutian disease of mink, mink is a kind of expensive fur economic animal. Using the detection method of fecal DNA extraction, it can not only avoid the influence and damage to mink, but also can accurately determine the infection of Aleutian disease. Therefore, this study uses the DNA extraction technique of mink feces to detect Aleutian virus in mink, and based on this, it discusses Aleutian. The main results were as follows: (1) using the Ezup column virus DNA extraction kit, the Aleutian virus DNA. was successfully extracted from the dung of the sable mink. The method could be used to detect Aleutian disease in mink. (2) the Aleutian virus (DNA) from the number of litter and the two groups of feces in the low two groups was expanded by PCR. After the increase, the electrophoresis results showed that the toxicity rate of the low yield array was higher than the high yield array. It showed that Aleutian disease had an impact on the number of mink litter, and the number of mink litter decreased. In summary, this study confirmed that the virus DNA extraction kit could extract Aleutian virus DNA in mink feces; this method can be used for the detection of Aleutian disease in Mink; Mink Aleutian disease affects mink litter size, resulting in a decrease in litter size in the affected mink.
【學(xué)位授予單位】：東北農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S858.92

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