發(fā)布時(shí)間：2018-06-12 14:45

  本文選題：抗菌肽 + 海蘭褐蛋雞��； 參考：《吉林大學(xué)》2016年碩士論文

【摘要】：本試驗(yàn)采用單因子設(shè)計(jì)方案。隨機(jī)選取480只健康、產(chǎn)蛋率相近的380日齡海蘭褐蛋雞,隨機(jī)分成2組,每組內(nèi)設(shè)3個(gè)重復(fù),每個(gè)重復(fù)80只雞。其中對(duì)照組飼喂基礎(chǔ)日糧,試驗(yàn)組在基礎(chǔ)日糧中添加0.01L/kg鱟素抗菌肽。預(yù)飼期7 d,試驗(yàn)期35 d。本研究通過比較兩個(gè)處理組蛋雞的生產(chǎn)性能、血液生化指標(biāo)、蛋品質(zhì)及蛋殼超微結(jié)構(gòu),探究鱟素抗菌肽對(duì)海蘭褐蛋雞產(chǎn)蛋后期的生產(chǎn)性能及蛋品質(zhì)的影響。在此基礎(chǔ)上,應(yīng)用RT-PCR技術(shù),檢測海蘭褐蛋雞子宮組織Ca BP-D28k m RNA的表達(dá)水平,進(jìn)一步研究鱟素抗菌肽對(duì)蛋品質(zhì)影響的分子作用機(jī)制。該研究對(duì)改善海蘭褐蛋雞蛋殼品質(zhì)具有一定的理論意義。假設(shè)該試驗(yàn)結(jié)果能在養(yǎng)雞業(yè)乃至家禽業(yè)生產(chǎn)中推廣應(yīng)用,對(duì)降低雞蛋破損率,提高養(yǎng)雞經(jīng)濟(jì)效益具有十分重要的實(shí)際意義。試驗(yàn)結(jié)果如下:1.生產(chǎn)性能:與對(duì)照組比較,試驗(yàn)組產(chǎn)蛋率顯著提高9.09%(P0.05),平均蛋重顯著增加6.25%(P0.05)。試驗(yàn)組較對(duì)照組料蛋比降低4.02%,破蛋率降低13.08%,差異不顯著(P0.05)。2.血清生化指標(biāo):與對(duì)照組相比,試驗(yàn)組總膽固醇顯著提高45.85%(P0.05),血鈣顯著提高21.59%(P0.05)。總蛋白提高2.81%,血磷提高21.93%,血糖降低11.35%,差異不顯著(P0.05)。3.蛋品質(zhì)指標(biāo):與對(duì)照組比較,試驗(yàn)組蛋殼強(qiáng)度顯著增加15.19%(P0.05)。試驗(yàn)組較對(duì)照組蛋殼厚度增加0.55%,蛋殼顏色增加2.86%,蛋殼相對(duì)重增加6.91%,哈氏單位增加5.44%,差異不顯著(P0.05)。4.蛋殼超微結(jié)構(gòu):與對(duì)照組相比,試驗(yàn)組蛋殼的表面角質(zhì)層皴裂紋少且裂隙小,試驗(yàn)組蛋殼的垂直晶體層及柵欄層結(jié)構(gòu)平整、致密,柵欄層氣孔少,乳頭個(gè)體大且突出,乳頭數(shù)目較少。5.Ca BP-D28K基因表達(dá)水平:與對(duì)照組相比,試驗(yàn)組蛋雞子宮組織Ca BP-D28k m RNA的表達(dá)水平極顯著提高(P=0.01)。結(jié)論:1.鱟素抗菌肽顯著提高了海蘭褐蛋雞產(chǎn)蛋后期的蛋重、產(chǎn)蛋率,并在一定程度上降低了破蛋率,提高了海蘭褐蛋雞的生產(chǎn)性能。2.鱟素抗菌肽顯著提高血清總膽固醇及血鈣的水平。3.鱟素抗菌肽顯著提高了海蘭褐蛋雞產(chǎn)蛋后期的蛋殼強(qiáng)度,并在一定程度上增加了蛋殼厚度,加深了蛋殼顏色、提高了蛋殼相對(duì)重和哈氏單位。4.鱟素抗菌肽減少蛋殼表面皴裂紋數(shù)量,降低裂隙之間的距離,并且蛋殼的均勻性較好,致密度增加�？v切面乳突顆粒數(shù)較少且乳頭個(gè)體較大。5.鱟素抗菌肽極顯著提高了海蘭褐蛋雞產(chǎn)蛋后期子宮組織Ca BP-D28k m RNA的表達(dá)水平。
[Abstract]:A single factor design scheme was used in this experiment. 480 healthy and 380 day old hens with similar laying rate were randomly divided into 2 groups. There were 3 repetitions in each group, and 80 chickens were repeated in each group. The control group was fed with basal diet. The experimental group added 0.01L/kg Limulus antibacterial peptide in the basal diet. The pre feeding period was 7 d, and the test period 35 D. passed through the study. The effects of the production performance, blood biochemical indexes, egg quality and eggshell ultrastructure of two treated groups were compared, and the effect of lysate antibacterial peptide on the production performance and egg quality in the later period of laying hens was investigated. On this basis, the expression level of Ca BP-D28k m RNA in the uterine tissue of hens was detected by RT-PCR technology, and the limulus lysate was further studied. The molecular mechanism of the effect of antibiotic peptide on egg quality. This study has a certain theoretical significance to improve the quality of eggshell egg shell. It is assumed that the test results can be applied in the poultry industry and poultry production. It has very important practical significance for reducing the egg breakage rate and raising the economic benefit of the chicken. The experimental results are as follows. 1. production performance: compared with the control group, the egg production rate of the experimental group was significantly increased by 9.09% (P0.05), the average egg weight was significantly increased by 6.25% (P0.05). Compared with the control group, the egg ratio of the experimental group decreased by 4.02%, the egg breaking rate was 13.08%, and the difference was not significant (P0.05).2. serum biochemical index: compared with the control group, the total cholesterol was significantly increased by 45.85% (P0.05), and the blood calcium showed a significant difference. The total protein increased by 21.59% (P0.05), the total protein increased by 2.81%, the blood phosphorus increased by 21.93%, the blood sugar decreased by 11.35%, the difference was not significant (P0.05).3. egg quality index: compared with the control group, the egg shell strength of the experimental group increased by 15.19% (P0.05). The experimental group increased the eggshell thickness by 0.55%, the eggshell color increased 2.86%, the eggshell relative weight increase 6.91%, hastelis unit. Increased 5.44%, the difference was not significant (P0.05).4. eggshell ultrastructure: compared with the control group, the surface cuticle cracks of the experimental group were less and the cracks were small. The vertical crystal layer and palisade layer structure of the experimental group were flat, dense, the stomata of the palisade layer were few, the nipple individuals were large and prominent, and the number of nipple was less.5.Ca BP-D28K gene expression level: and pairs of pairs. Compared with the experimental group, the expression level of Ca BP-D28k m RNA in the uterus tissue of the experimental group was greatly improved (P=0.01). Conclusion: 1. Limulus antibacterial peptide significantly improved the egg weight, the egg production rate, and reduced the egg breaking rate to a certain extent, and improved the production performance of the hens by.2.. The level of.3. in cholesterol and blood calcium significantly increased the shell strength in the later period of laying hens, and to a certain extent increased the thickness of the eggshell, deepened the color of the shell, improved the relative weight of the shell and the.4. limulus lysate, reduced the number of chapped lines on the surface of the eggshell, and reduced the distance between the cracks, and the eggshell. The homogeneity was better and the density increased. The number of mastoid granules in the longitudinal section was less and the large.5. Limulus antiseptic peptide significantly improved the expression level of Ca BP-D28k m RNA in the later period of the egg production of hens.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：S831

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