本文選題：基因C型鴨甲肝病毒 + 間接免疫熒光技術(shù)　； 參考：《中國(guó)預(yù)防獸醫(yī)學(xué)報(bào)》2017年04期

【摘要】：為建立基因C型鴨甲肝病毒(DHAV-C)間接免疫熒光檢測(cè)技術(shù)(IFA),本研究以純化的DHAV-C單克隆抗體(MAb)為一抗,FITC標(biāo)記的兔抗鼠Ig G(Ig G-FITC)為二抗,建立了DHAV-C IFA檢測(cè)方法。該方法僅對(duì)感染DHAV-C的肝組織觸片檢測(cè)為陽(yáng)性,而對(duì)分別感染基因A型鴨甲肝病毒、新城疫病毒、鴨瘟病毒、鴨圓環(huán)病毒、鴨源金黃色葡萄球菌、鴨源大腸桿菌、鴨源多殺性巴氏桿菌、鴨源沙門氏菌的SPF鴨肝組織觸片檢測(cè)為陰性,表明該方法特異性好;重復(fù)性試驗(yàn)表明,該方法批內(nèi)和批間重復(fù)性好。一抗和二抗在-20℃至少能保存7個(gè)月。實(shí)驗(yàn)感染SPF雛鴨IFA和RT-PCR檢測(cè)符合率100%。本研究建立的DHAV-C IFA方法特異性好,可以用于該病毒感染肝組織的快速檢測(cè)。
[Abstract]:In order to establish an indirect immunofluorescence assay for detection of DHAV-C gene type C duck hepatitis A virus (DHAV-C), a new method for detection of DHAV-C IFA was developed, using purified DHAV-C monoclonal antibody (MAb) as a second antibody against rabbit anti-FITC labeled rabbit anti-mouse Ig G G FITC. The method was only positive for DHAV-C infected liver tissue, but positive for duck hepatitis A virus, Newcastle disease virus, duck plague virus, duck circovirus, duck Staphylococcus aureus, duck Escherichia coli. The detection of Pasteurella multocida from duck and salmonella from duck was negative, which indicated that the method was specific and reproducible, and the repeatability test showed that the method had good reproducibility within and between batches. The first and second antibodies can be preserved at-20 鈩，

本文編號(hào)：2009711