本文選題：狂犬病病毒 + G蛋白　； 參考：《河南農(nóng)業(yè)科學(xué)》2017年04期

【摘要】：為優(yōu)化狂犬病病毒G蛋白的原核表達(dá)條件,探究其反應(yīng)原性,參照狂犬病病毒CTN-1V10株編碼G蛋白基因序列,采用生物工程合成的方法合成編碼G蛋白的基因片段,并將該片段命名為G5F。將該片段與pET-28a原核表達(dá)載體連接,構(gòu)建重組表達(dá)質(zhì)粒pET-G5F,并將重組質(zhì)粒進(jìn)行誘導(dǎo)表達(dá),在IPTG濃度為0.2 mmol/L、溫度為20℃、誘導(dǎo)12 h時(shí),重組蛋白表達(dá)量最高。SDS-PAGE電泳及Western blot分析表明,G5F重組蛋白可溶性表達(dá)量高,且可以被抗狂犬病病毒單克隆抗體識(shí)別,反應(yīng)原性良好。
[Abstract]:In order to optimize the prokaryotic expression conditions of rabies virus G protein and to explore its reactivity, the gene fragment encoding G protein was synthesized by bioengineering method according to the coding G protein gene sequence of rabies virus CTN-1V10 strain. The fragment was named G5F. The recombinant plasmid pET-G5F was constructed by ligating the fragment with the prokaryotic expression vector pET-28a. The recombinant plasmid was induced to express at 0.2 mmol / L IPTG at 20 鈩，

本文編號(hào)：2005282