本文選題：脫氧血腐鐮刀菌烯醇 + 樹(shù)突狀細(xì)胞　； 參考：《南京農(nóng)業(yè)大學(xué)學(xué)報(bào)》2017年02期

【摘要】：[目的]本試驗(yàn)旨在研究脫氧血腐鐮刀菌烯醇(deoxynivalenol,DON)對(duì)脂多糖(lipopolysaccharide,LPS)刺激的小鼠骨髓源性成熟樹(shù)突狀細(xì)胞(mature dendritic cell,m DC)的表型和吞噬功能的影響。[方法]體外培養(yǎng)小鼠骨髓細(xì)胞誘導(dǎo)分化成未成熟的樹(shù)突狀細(xì)胞,分別用不同質(zhì)量濃度的DON(0、125、250、500 ng·m L~(-1))處理DC 6 h后,再加入LPS刺激18 h。以?xún)H用LPS處理的為陽(yáng)性對(duì)照組。用流式細(xì)胞儀檢測(cè)DC表型(CD80、CD86和MHC-Ⅱ)變化及吞噬右旋葡聚糖的能力;RTPCR檢測(cè)細(xì)胞中細(xì)胞因子(IL-6、IL-0、IL-12和TNF-α)mRNA的相對(duì)表達(dá)。[結(jié)果]LPS刺激引起m DC表面的CD80、CD86和MHC-Ⅱ升高(P0.05),吞噬右旋葡聚糖能力下降,IL-6、IL-0、IL-12和TNF-α的mRNA相對(duì)表達(dá)水平提高(P0.05);低濃度和中濃度DON(125和250 ng·m L~(-1))對(duì)DC的表型和免疫功能沒(méi)有影響(P0.05),高濃度DON(500 ng·m L~(-1))引起m DC共刺激分子(CD80、CD86和MHC-Ⅱ)表達(dá)水平降低(P0.05),同時(shí)細(xì)胞因子的mRNA表達(dá)降低(P0.05),但吞噬能力提高12.2%。[結(jié)論]高濃度DON對(duì)LPS刺激的m DC表型和細(xì)胞因子表達(dá)具有一定的抑制作用。
[Abstract]:[objective] to investigate the effects of deoxynivalenol (DON) on the phenotype and phagocytosis of murine bone marrow-derived mature dendritic cells (BMSCs) stimulated by lipopolysaccharide (LPS). [methods] Murine bone marrow cells were induced to differentiate into immature dendritic cells in vitro. DC were treated with different concentrations of DONX 0125 250500 ng / L (1 / 1) for 6 h and then stimulated by LPS for 18 h. The positive control group was treated with LPS only. The changes of CD80 CD86 and MHC- 鈪，

本文編號(hào)：2005127