本文選題：旋毛蟲(chóng) + 排泄分泌物��； 參考：《吉林大學(xué)》2015年碩士論文

【摘要】：旋毛蟲(chóng)病是一種重要的食源性人畜共患寄生蟲(chóng)病，呈世界性分布，由組織棲線形旋毛蟲(chóng)引起。人類(lèi)通過(guò)生食或半生食含旋毛蟲(chóng)肌幼蟲(chóng)的肉類(lèi)（主要是豬肉）而感染旋毛蟲(chóng)病。此病目前在一些發(fā)展中國(guó)家（如泰國(guó)、阿根廷及中國(guó)等）仍常有暴發(fā)，究其原因除了缺少有效的預(yù)防控制措施外，關(guān)鍵在于至今沒(méi)有良好的診斷方法。旋毛蟲(chóng)病的臨床癥狀表現(xiàn)一般不具明顯特征，且易與其它傳染病相混淆，因此臨床診斷較為困難。雖然肌肉活檢發(fā)現(xiàn)幼蟲(chóng)或包囊即可確診，但在輕度感染早期往往不易檢出，即使是感染后期，因受組織局限性影響，活檢陽(yáng)性率也僅有50%左右。為此，尋找一種敏感性高、特異性強(qiáng)的檢測(cè)診斷方法對(duì)于旋毛蟲(chóng)病的診、防、治具有十分重要的意義，其關(guān)鍵是篩選到有效的免疫學(xué)檢測(cè)診斷抗原。 本研究擬以小鼠為實(shí)驗(yàn)動(dòng)物模型，并應(yīng)用于臨床出欄豬的診斷研究，利用中國(guó)河南豬旋毛蟲(chóng)T1分離株(ISS534)分別感染小鼠和豬，采用旋毛蟲(chóng)不同發(fā)育時(shí)期的排泄分泌物抗原（ES）作為診斷抗原，應(yīng)用ELISA方法檢測(cè)感染小鼠和豬60天內(nèi)不同天數(shù)的血清抗旋毛蟲(chóng)IgM和IgG抗體水平，并采用Excel軟件繪制其抗體消長(zhǎng)規(guī)律曲線并進(jìn)行數(shù)據(jù)分析。結(jié)果表明：對(duì)于小鼠模型，，三種抗原檢測(cè)到抗旋毛蟲(chóng)抗體IgM均成“波峰波谷”形式的變化曲線，可以利用旋毛蟲(chóng)三個(gè)發(fā)育時(shí)期ES抗原的混合物來(lái)檢測(cè)旋毛蟲(chóng)45天前的感染（即5-14天的感染檢測(cè)IgM、15-45天檢測(cè)IgG），感染45天之后可以利用單一肌幼蟲(chóng)時(shí)期ES抗原檢測(cè)IgG，最有意義的發(fā)現(xiàn)是利用旋毛蟲(chóng)成蟲(chóng)期和新生幼蟲(chóng)期的ES抗原可以有效檢測(cè)旋毛蟲(chóng)的早期感染；對(duì)于豬的應(yīng)用研究，低劑量感染最好利用旋毛蟲(chóng)不同發(fā)育時(shí)期的3至5種分泌物抗原的混合物來(lái)檢測(cè)旋毛蟲(chóng)2個(gè)月前的感染；中高劑量感染可利用單一感染后10小時(shí)腸道期肌幼蟲(chóng)ES抗原來(lái)檢測(cè)感染60天內(nèi)血清抗旋毛蟲(chóng)IgM抗體。本研究成功獲悉旋毛蟲(chóng)感染宿主后不同發(fā)育時(shí)期刺激機(jī)體產(chǎn)生抗旋毛蟲(chóng)抗體的消長(zhǎng)規(guī)律，確定了旋毛蟲(chóng)不同發(fā)育時(shí)期的免疫學(xué)診斷抗原，為進(jìn)一步合理利用旋毛蟲(chóng)不同發(fā)育時(shí)期的排泄分泌物抗原建立起有效檢測(cè)旋毛蟲(chóng)感染的診斷制劑的研制提供了重要的理論基礎(chǔ)和科學(xué)依據(jù)。
[Abstract]:Trichinellosis is an important zoonotic parasitic disease caused by histopathic nematode. Humans are infected with trichinellosis by eating raw or semi-raw meat (mainly pork) containing the muscle larvae of Trichinella spiralis. There are still many outbreaks in some developing countries (such as Thailand, Argentina and China). Besides the lack of effective prevention and control measures, the key lies in the lack of good diagnostic methods. The clinical symptoms of trichinellosis are not obvious and easily confused with other infectious diseases, so clinical diagnosis is difficult. Although the larva or cyst can be diagnosed by muscle biopsy, it is not easy to detect in the early stage of mild infection. Even in the late stage of infection, the positive rate of biopsy is only about 50% due to the influence of tissue localization. Therefore, it is of great significance for the diagnosis, prevention and treatment of trichinellosis to find a highly sensitive and specific method for detection and diagnosis. The key is to screen effective immunological diagnostic antigens. This study was designed to use mice as an experimental animal model and to be used in the clinical diagnosis of pigs. The mice and pigs were infected with ISS534, a T1 strain of Trichinella spiralis from Henan Province, China, respectively. The excretory secretion antigens of Trichinella spiralis were used as diagnostic antigens. Elisa was used to detect the levels of anti-trichinella IgM and IgG in sera of infected mice and pigs for different days within 60 days. Excel software was used to draw the curve of antibody growth and decline and to analyze the data. The results showed that for the mouse model, the three antigens detected the change curve of anti-trichinella antibody IgM in the form of "peak and trough". The mixture of es antigens of three developmental stages of Trichinella spiralis can be used to detect the infection of Trichinella spiralis 45 days ago (i.e. 5-14 days of infection detection of IgMN 15-45 days of detection of IgGN). After 45 days of infection, the single muscle larvae period es antigen can be used to detect IgGs. It is significant to find that the early infection of Trichinella spiralis can be detected effectively by using es antigens of adult and newborn larvae. For the application study of pigs, low dose infection is best to detect trichinella infection 2 months ago by using a mixture of 3 to 5 secretions antigens of Trichinella spiralis at different developmental stages. The serum anti-trichinella IgM antibody could be detected by using the es antigen of the intestinal muscle larvae 10 hours after a single infection in the middle and high dose of infection. In this study, we have learned the law of the growth and decline of anti-trichinella antibody in different development period after Trichinella spiralis infection, and determined the immunological diagnosis antigen of Trichinella spiralis in different development period. It provides an important theoretical basis and scientific basis for the development of an effective diagnostic preparation for the detection of Trichinella spiralis infection by using the excretory secretion antigens of Trichinella spiralis at different developmental stages.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：S852.7

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本文編號(hào)：2004854