本文選題：旋毛蟲 + 排泄分泌物　； 參考：《吉林大學(xué)》2015年碩士論文

【摘要】：旋毛蟲病是一種重要的食源性人畜共患寄生蟲病，呈世界性分布，由組織棲線形旋毛蟲引起。人類通過生食或半生食含旋毛蟲肌幼蟲的肉類（主要是豬肉）而感染旋毛蟲病。此病目前在一些發(fā)展中國家（如泰國、阿根廷及中國等）仍常有暴發(fā)，究其原因除了缺少有效的預(yù)防控制措施外，關(guān)鍵在于至今沒有良好的診斷方法。旋毛蟲病的臨床癥狀表現(xiàn)一般不具明顯特征，且易與其它傳染病相混淆，因此臨床診斷較為困難。雖然肌肉活檢發(fā)現(xiàn)幼蟲或包囊即可確診，但在輕度感染早期往往不易檢出，即使是感染后期，因受組織局限性影響，活檢陽性率也僅有50%左右。為此，尋找一種敏感性高、特異性強的檢測診斷方法對于旋毛蟲病的診、防、治具有十分重要的意義，其關(guān)鍵是篩選到有效的免疫學(xué)檢測診斷抗原。 本研究擬以小鼠為實驗動物模型，并應(yīng)用于臨床出欄豬的診斷研究，利用中國河南豬旋毛蟲T1分離株(ISS534)分別感染小鼠和豬，采用旋毛蟲不同發(fā)育時期的排泄分泌物抗原（ES）作為診斷抗原，應(yīng)用ELISA方法檢測感染小鼠和豬60天內(nèi)不同天數(shù)的血清抗旋毛蟲IgM和IgG抗體水平，并采用Excel軟件繪制其抗體消長規(guī)律曲線并進行數(shù)據(jù)分析。結(jié)果表明：對于小鼠模型，，三種抗原檢測到抗旋毛蟲抗體IgM均成“波峰波谷”形式的變化曲線，可以利用旋毛蟲三個發(fā)育時期ES抗原的混合物來檢測旋毛蟲45天前的感染（即5-14天的感染檢測IgM、15-45天檢測IgG），感染45天之后可以利用單一肌幼蟲時期ES抗原檢測IgG，最有意義的發(fā)現(xiàn)是利用旋毛蟲成蟲期和新生幼蟲期的ES抗原可以有效檢測旋毛蟲的早期感染；對于豬的應(yīng)用研究，低劑量感染最好利用旋毛蟲不同發(fā)育時期的3至5種分泌物抗原的混合物來檢測旋毛蟲2個月前的感染；中高劑量感染可利用單一感染后10小時腸道期肌幼蟲ES抗原來檢測感染60天內(nèi)血清抗旋毛蟲IgM抗體。本研究成功獲悉旋毛蟲感染宿主后不同發(fā)育時期刺激機體產(chǎn)生抗旋毛蟲抗體的消長規(guī)律，確定了旋毛蟲不同發(fā)育時期的免疫學(xué)診斷抗原，為進一步合理利用旋毛蟲不同發(fā)育時期的排泄分泌物抗原建立起有效檢測旋毛蟲感染的診斷制劑的研制提供了重要的理論基礎(chǔ)和科學(xué)依據(jù)。
[Abstract]:Trichinellosis is an important zoonotic parasitic disease caused by histopathic nematode. Humans are infected with trichinellosis by eating raw or semi-raw meat (mainly pork) containing the muscle larvae of Trichinella spiralis. There are still many outbreaks in some developing countries (such as Thailand, Argentina and China). Besides the lack of effective prevention and control measures, the key lies in the lack of good diagnostic methods. The clinical symptoms of trichinellosis are not obvious and easily confused with other infectious diseases, so clinical diagnosis is difficult. Although the larva or cyst can be diagnosed by muscle biopsy, it is not easy to detect in the early stage of mild infection. Even in the late stage of infection, the positive rate of biopsy is only about 50% due to the influence of tissue localization. Therefore, it is of great significance for the diagnosis, prevention and treatment of trichinellosis to find a highly sensitive and specific method for detection and diagnosis. The key is to screen effective immunological diagnostic antigens. This study was designed to use mice as an experimental animal model and to be used in the clinical diagnosis of pigs. The mice and pigs were infected with ISS534, a T1 strain of Trichinella spiralis from Henan Province, China, respectively. The excretory secretion antigens of Trichinella spiralis were used as diagnostic antigens. Elisa was used to detect the levels of anti-trichinella IgM and IgG in sera of infected mice and pigs for different days within 60 days. Excel software was used to draw the curve of antibody growth and decline and to analyze the data. The results showed that for the mouse model, the three antigens detected the change curve of anti-trichinella antibody IgM in the form of "peak and trough". The mixture of es antigens of three developmental stages of Trichinella spiralis can be used to detect the infection of Trichinella spiralis 45 days ago (i.e. 5-14 days of infection detection of IgMN 15-45 days of detection of IgGN). After 45 days of infection, the single muscle larvae period es antigen can be used to detect IgGs. It is significant to find that the early infection of Trichinella spiralis can be detected effectively by using es antigens of adult and newborn larvae. For the application study of pigs, low dose infection is best to detect trichinella infection 2 months ago by using a mixture of 3 to 5 secretions antigens of Trichinella spiralis at different developmental stages. The serum anti-trichinella IgM antibody could be detected by using the es antigen of the intestinal muscle larvae 10 hours after a single infection in the middle and high dose of infection. In this study, we have learned the law of the growth and decline of anti-trichinella antibody in different development period after Trichinella spiralis infection, and determined the immunological diagnosis antigen of Trichinella spiralis in different development period. It provides an important theoretical basis and scientific basis for the development of an effective diagnostic preparation for the detection of Trichinella spiralis infection by using the excretory secretion antigens of Trichinella spiralis at different developmental stages.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S852.7

【參考文獻】

相關(guān)期刊論文 前8條

1 申麗潔,黎世濤,王秀珍,朱聲華,羅仲金;旋毛蟲三種抗原作為免疫診斷抗原的比較研究[J];地方病通報;1999年02期

2 申麗潔,申元英,朱聲華;旋毛蟲成蟲可溶性抗原SDS-PAGE及Western-blot分析[J];大理醫(yī)學(xué)院學(xué)報;2000年01期

3 劉紫金,王超峰,葛峰,司建新;豬旋毛蟲檢驗方法探析[J];肉品衛(wèi)生;2004年06期

4 任紅斌,張海珠,劉世國,史明珠;旋毛蟲感染早期兔血清中IgM的檢測[J];新鄉(xiāng)醫(yī)學(xué)院學(xué)報;1997年03期

5 朱名勝,劉文獻;斑點 ELISA法診斷人、鼠旋毛蟲病的研究[J];中國寄生蟲病防治雜志;2000年02期

6 王潔;崔晶;王中全;;小鼠實驗感染不同種旋毛蟲后血清IgG抗體水平的變化[J];中國病原生物學(xué)雜志;2007年04期

7 張國華,牛安歐,胡昌仁;應(yīng)用PCR檢測旋毛蟲DNA的研究[J];中國寄生蟲病防治雜志;1997年03期

8 朱名勝,朱艷霞,劉文獻,貝濤,常向東;Dot-ELISA法和IEST檢測旋毛蟲抗體的實驗研究[J];中國人獸共患病雜志;2001年05期

本文編號：2004854