本文選題：肝臟 + 脾臟�。� 參考：《內(nèi)蒙古農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：本論文旨在研究限飼及不同方式補(bǔ)飼對蒙古公羔羊肝、脾、胰腺、結(jié)腸的形態(tài)結(jié)構(gòu)及空腸和肝臟相關(guān)基因表達(dá)的影響。選用30只四月齡蒙古公羔羊,平均體重為20.70±3.51kg,隨機(jī)平均分為對照組(CG)和限制組(RG)。試驗期分限制飼養(yǎng)(0-60d)和補(bǔ)償飼養(yǎng)(61-120d)。限制期結(jié)束,從兩組中隨機(jī)選五只屠宰采樣。每組剩余隨機(jī)分成兩組,青干草組(CH、RH)和秸稈組(CM、RM),青干草組飼喂精粗比為5:5的青干草精料日糧,秸稈組飼喂精粗比3:7的秸稈精料日糧,補(bǔ)償期結(jié)束后,全部屠宰采樣。采用組織切片法研究肝、脾、胰腺、結(jié)腸形態(tài)結(jié)構(gòu)變化,對空腸及肝臟進(jìn)行實時熒光定量PCR檢測代謝相關(guān)基因變化,從而對限制和補(bǔ)償生長對羊空腸結(jié)腸及相關(guān)實質(zhì)器官的影響做進(jìn)一步研究。結(jié)果表明:試驗一結(jié)果:(1)本試驗條件下蒙古公羔羊結(jié)腸的結(jié)果:結(jié)腸黏膜層代謝旺盛,營養(yǎng)限制—段時間,由于動物機(jī)體趨于吸收更多能量,黏膜層厚度代償性地升高。(2)本試驗條件下蒙古公羔羊肝臟的結(jié)果:通過60天限制飼養(yǎng),RG組肝細(xì)胞周長顯著低于CG組。而60天營養(yǎng)補(bǔ)償后,肝臟細(xì)胞周長恢復(fù)到正常水平。肝臟中央靜脈周長、肝細(xì)胞核周長恢復(fù)正常。(3)本試驗條件下蒙古公羔羊脾臟的結(jié)果:營養(yǎng)限制與補(bǔ)償對脾臟白髓影響不明顯,可能當(dāng)發(fā)生免疫應(yīng)答反應(yīng)后脾白髓會出現(xiàn)顯著的增大。(4)本試驗條件下蒙古公羔羊胰腺的結(jié)果:胰腺腺泡周長、胰腺灰度值,營養(yǎng)限制期與補(bǔ)償期后變化不顯著。試驗二結(jié)果:(1)空腸基因表達(dá)的變化:本試驗條件下,高精料補(bǔ)償有助于Claudin-1基因mRNA表達(dá),提高了細(xì)胞屏障作用。營養(yǎng)限制60天,限飼組Caveolin-1基因mRNA表達(dá)量高于對照組3.27倍。營養(yǎng)補(bǔ)償60天后,可能由于Caveolin-1基因在限飼后基因表達(dá)代償性顯著升高,而補(bǔ)償后恢復(fù)到正常水平。營養(yǎng)限制與補(bǔ)償對空腸connexin-43基因的表達(dá)沒有影響。(2)肝臟脂肪代謝基因的變化:營養(yǎng)限制后PPARγγ基因mRNA表達(dá)顯著高于對照組,營養(yǎng)限制導(dǎo)致脂肪分解,肝臟合成甘油三酯進(jìn)行儲存。營養(yǎng)限制后FASN和ACACA基因的表達(dá)量顯著降低,主要原因是由于乙酸的降低,高精料沒有促進(jìn)FASN和ACACA基因的表達(dá)顯著升高。
[Abstract]:The purpose of this study was to investigate the effects of dietary restriction and supplementation on the morphological structure of liver, spleen, pancreas and colon and the expression of genes related to jejunum and liver in Mongolian male lambs. Thirty four-month-old Mongolian male lambs with an average weight of 20.70 鹵3.51 kg were randomly divided into control group (CGG) and restriction group (RGG). During the trial period, the feeding period was limited to 0-60 days) and the compensatory feeding time was 61-120 days. At the end of the restriction period, five slaughter samples were randomly selected from the two groups. Each group was randomly divided into two groups: the green hay group (CHRH) and the straw group (CMR RMN), the green hay group fed with the concentrate diet of the crude ratio of 5:5 and the straw group fed with the concentrate ration of the concentrate at 3:7. After the compensation period was over, all the samples were slaughtered. The morphologic and structural changes of liver, spleen, pancreas and colon were studied by tissue section method. The changes of metabolism-related genes in jejunum and liver were detected by real-time fluorescence quantitative PCR. The effects of limiting and compensating growth on jejunal colon and related parenchymal organs were further studied. The results showed that under the condition of this experiment, the colon of Mongolian male lambs was metabolized vigorously, nutrition was limited for a period of time, and the animal body tended to absorb more energy. The results of the experiment showed that the liver circumference of Mongolian male lambs was significantly lower than that of CG group after 60 days of restriction. However, after 60 days of nutrition compensation, the peripheral length of liver cells returned to normal level. The results of this study were as follows: nutrition restriction and compensation had no significant effect on white pulp of spleen. The results of this study were as follows: the acinar circumference of the pancreas, the gray value of the pancreas, the nutritional limitation period and the compensation period had no significant changes. The results of experiment 2: the change of gene expression in jejunum: under this condition, high concentrate compensation was helpful to Claudin-1 gene mRNA expression and enhanced cell barrier. The mRNA expression of Caveolin-1 gene was 3.27 times higher in diet restriction group than that in control group for 60 days. After 60 days of nutrition compensation, Caveolin-1 gene expression increased significantly after feeding restriction, but returned to normal level after compensation. Nutrition restriction and compensation had no effect on the expression of connexin-43 gene in jejunum. After nutrition restriction, the expression of FASN and ACACA genes decreased significantly, mainly due to the decrease of acetic acid, and the high concentrate did not promote the expression of FASN and ACACA genes.
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S826

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