發(fā)布時(shí)間：2018-06-09 05:22

  本文選題：哺乳仔豬 + 微生態(tài)制劑　； 參考：《南京農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：哺乳仔豬由于免疫系統(tǒng)、消化系統(tǒng)功能不完善,外界環(huán)境壓力等原因常導(dǎo)致腹瀉。而腹瀉會(huì)對(duì)仔豬的生長(zhǎng)性能和免疫系統(tǒng)產(chǎn)生重大影響。微生態(tài)制劑作為抗生素替代品,已開(kāi)始應(yīng)用于預(yù)防和控制仔豬腹瀉,但其對(duì)仔豬的生長(zhǎng)性能、腹瀉率和血液指標(biāo)的影響及其適宜劑量還需進(jìn)一步研究。本文旨在研究,給腹瀉和未腹瀉的仔豬灌服不同劑量的糞腸球菌微生物制劑,觀察其對(duì)仔豬的生長(zhǎng)性能(主要是指仔豬日增重)和血液指標(biāo)的影響。1腹瀉仔豬血液常規(guī)、生化指標(biāo)情況本實(shí)驗(yàn)選擇胎次、產(chǎn)期、產(chǎn)仔數(shù)相近的新生仔豬48窩,第一階段是在仔豬出生后,細(xì)心觀察仔豬群腹瀉狀況,一旦發(fā)現(xiàn)某一窩有仔豬發(fā)生腹瀉現(xiàn)象,立即將該腹瀉仔豬及同窩同性別未腹瀉的仔豬各1頭,進(jìn)行稱重、屠宰、采集,直至采集20窩;第二階段,將這20窩仔豬隨機(jī)分對(duì)照組(C)、試驗(yàn)組1(T1)、試驗(yàn)組2(T2)、試驗(yàn)組3(T3)、試驗(yàn)組4(T4),每組4窩。五個(gè)組于腹瀉當(dāng)日(0d)、第3日(3d)、第6日(6d)全窩分別灌服雙蒸水1 mL(C)、糞腸球菌2.5 × 108CFU/mL(T1)、5× 108CFU/mL(T2)、1 × 109CFU/mL(T3)、2 × 109CFU/mL(T4)的微生態(tài)制劑 1mL。到20日齡時(shí),每窩選擇試驗(yàn)期間發(fā)生過(guò)腹瀉與同性別從未腹瀉的仔豬各一頭稱重、采集樣品分析腹瀉仔豬血液常規(guī)、生化指標(biāo)情況。結(jié)果表明:腹瀉仔豬組血液總蛋白(TP)、球蛋白(GLO)均顯著高于未腹瀉組(P0.05);腹瀉仔豬組血液常規(guī)指標(biāo)與未腹瀉仔豬組間無(wú)顯著差異。2灌服微生態(tài)制劑對(duì)仔豬生長(zhǎng)性能、腹瀉率及血液指標(biāo)的影響本實(shí)驗(yàn)研究灌服微生態(tài)制劑對(duì)仔豬日增重、腹瀉率、血液常規(guī)以及血液生化指標(biāo)的影響。結(jié)果表明:灌服高劑量微生態(tài)制劑組(T3組,1×109及T4組,2×109)仔豬平均日增重極顯著高于對(duì)照組(P0.01)和低劑量微生態(tài)制劑組(P0.01),且灌服2 × 109的仔豬平均日增重極顯著高于1 × 109組(P0.01),而低劑量組與對(duì)照組相比無(wú)差異。與對(duì)照組相比,灌服微生態(tài)制劑對(duì)血液球蛋白(GLO)和A/G均有顯著影響(P0.05),其中灌喂高劑量微生態(tài)制劑的處理組4(T4)血液球蛋白(GLO)顯著高于對(duì)照組C以及其他處理組(P0.05),灌喂高劑量微生態(tài)制劑的T4組和低劑量的T1組的A/G顯著低于對(duì)照組(P0.05),且T4組中A/G顯著低于T2、T3組;與對(duì)照組相比,各處理組血液尿素氮(BUN)的含量有下降的趨勢(shì)(P0.1);腹瀉對(duì)血液球蛋白(GLO)和A/G也有顯著影響,腹瀉組中血液球蛋白(GLO)顯著低于未腹瀉組,A/G值顯著高于未腹瀉組A/G。灌服微生態(tài)制劑對(duì)仔豬血常規(guī)指標(biāo)無(wú)顯著影響(P0.05)。
[Abstract]:Lactation piglets often suffer from diarrhea due to immune system, imperfect digestive system function and external environmental pressure. Diarrhea can have a significant impact on the performance and immune system of piglets. As a substitute for antibiotics, microecological agents have been used to prevent and control diarrhea in piglets. However, the effects of microecological agents on growth performance, diarrhea rate, blood index and their appropriate dosage of piglets need to be further studied. The purpose of this study was to study the effects of different doses of Enterococcus faecalis microorganism on growth performance (mainly daily gain of piglets) and blood index of diarrhea piglets. Biochemical parameters the experiment selected 48 litter of newborn piglets with similar birth order, delivery period and litter size. The first stage was to observe the diarrhea status of piglets carefully after the birth of piglets. Once a litter of piglets was found to have diarrhea phenomenon, the first stage was to observe the diarrhea status of piglets carefully after the birth of piglets. Immediately weighing, slaughtering and collecting the diarrhoea piglets and 1 piglet of the same litter and not having diarrhea of the same sex until the collection of 20 litter; the second stage, The 20 piglets were randomly divided into control group (n = 1), test group (n = 2), test group (n = 3) and control group (n = 4). The microecological agents of the five groups were 1 mL of double distilled water and 2.5 脳 10 8 CFU / mL T1, 5 脳 108 CFU / mLT2 / 1 脳 10 9 CFU / mLT3 / 2 脳 109 CFU / mLT4, respectively, in the whole nest on the day of diarrhea, 3 days on the 3rd day and 6 days after the 6th day of diarrhoea (n = 6), and the microecologic agent of Enterococcus faecalis was 2.5 脳 10 8 CFU / m L ~ (-1) T _ (1) / T _ (2) 脳 10 ~ (9) CFU / m ~ (L) T _ (4). At the age of 20 days, one piglet with diarrhea and one piglet of the same sex who had never had diarrhea occurred during each litter selection trial. The blood routine and biochemical indexes of the piglets with diarrhea were collected and analyzed. The results showed that the levels of serum total protein (TPN) and globulin (GLOL) in diarrhea piglets were significantly higher than those in non-diarrhea piglets (P 0.05), and there was no significant difference between diarrhea piglets and diarrhea piglets. 2. The growth performance of piglets treated with microecological preparations was not significantly different from that of diarrhea piglets. The effect of microecological preparation on daily gain, diarrhea rate, blood routine and blood biochemical indexes of piglets. The results showed that the average daily gain (ADG) of the high dose microecological preparation group was significantly higher than that of the control group (P 0.01) and the low dose microecological preparation group (P 0.01), and the average daily gain of the piglets treated with 2 脳 10 9 was significantly higher than that of the control group (P 0.01) and the average daily gain of the piglets treated with 2 脳 10 9 were significantly higher than that of the control group (P 0.01). There was no difference between the low dose group and the control group. Compared with the control group, The effects of microecological preparations on blood globulin (GLOL) and A / G ratio were significant (P 0.05). The blood globulin GLOs of the treatment group fed with high dose microecological preparations were significantly higher than those of the control group C and other treatment groups (P 0.05), and were fed with high dose microecology. The Ar / G of T4 group and T1 group were significantly lower than that of control group (P 0.05), and Ar / G of T4 group was significantly lower than that of T2T 3 group. Compared with the control group, the blood urea nitrogen (bun) content of each treatment group was decreased (P _ (0.1), and diarrhea had a significant effect on the blood globulin GLOs and A- / -G, and the blood globulin G _ (g) in diarrhea group was significantly lower than that in the non-diarrhea group (P _ (0.1), and the value of blood globulin G _ (g) in diarrhea group was significantly higher than that in non-diarrhea group. There was no significant effect of microecological preparation on blood routine index of piglets.
【學(xué)位授予單位】：南京農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S858.28

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