本文選題：溶血巴斯德菌 + Sr�。� 參考：《西南農(nóng)業(yè)學(xué)報》2017年06期

【摘要】：【目的】探討雞蛋中溶血性微生物進來源及生物安全性�！痉椒ā坎捎锰禺愋耘囵B(yǎng)基在雞蛋中分離一株溶血性微生物,對其進行生化鑒定、16Sr DNA進行測序并聚類分析,采用7類9種抗生素抗性試驗對溶血微生物進行藥敏試驗,對其進行發(fā)酵底物蛋白質(zhì)和游離氨基酸研究�！窘Y(jié)果】生化鑒定與16r DNA鑒定分離的溶血性微生為溶血巴斯德菌,藥敏試驗結(jié)果表明氨基糖苷類抗生素對此菌有一定的抑制作用,但青霉素鈉、羅紅霉素和喹諾酮類抗生素環(huán)丙沙星對此菌無抑制效果,底物發(fā)酵實驗結(jié)果為溶血巴斯德菌的代謝對蛋白質(zhì)總量影響不大,但可升高發(fā)酵培養(yǎng)基中部分游離氨基酸的含量。【結(jié)論】雞蛋中存在非常見致病微生物的污染,并且部分抗生素對致病微生物已無抑制作用,雞蛋中的微生物可以改變底物結(jié)構(gòu),相關(guān)部門應(yīng)加強對新鮮雞蛋微生物污染的控制,關(guān)注罕見致病菌及條件致病菌引起的危害。
[Abstract]:[objective] to investigate the origin and biological safety of hemolytic microorganism in eggs. [methods] A hemolytic microorganism was isolated from egg by specific medium, and its 16Sr DNA was identified by sequencing and cluster analysis. Nine antibiotic resistance tests were used to study the susceptibility of hemolytic microorganisms to the fermentation substrate protein and free amino acids. [results] Hemolytic microorganism isolated by biochemical identification and 16r DNA identification was Pasteurella haemolyticus. The results of drug sensitivity test showed that aminoglycoside antibiotics could inhibit the bacteria to some extent, but penicillin sodium, roxithromycin and quinolone antibiotics ciprofloxacin had no inhibitory effect on the bacteria. The results of substrate fermentation showed that the metabolism of Pasteurella hemolyticus had little effect on the total protein content, but it could increase the content of some free amino acids in the fermentation medium. [conclusion] the contamination of pathogenic microorganisms was found in eggs. Some antibiotics have no inhibitory effect on pathogenic microorganisms, and microbes in eggs can change the structure of substrate. The relevant departments should strengthen the control of microbial contamination in fresh eggs and pay attention to the damage caused by rare pathogenic bacteria and conditioned pathogens.
【作者單位】： 四川省農(nóng)業(yè)科學(xué)院分析測試中心;
【基金】：國家農(nóng)產(chǎn)品質(zhì)量安全風險評估重大專項(GJFP201601302)
【分類號】：S852.61

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