本文選題：芽囊原蟲 + 珍稀珍稀動物�。� 參考：《西北農(nóng)林科技大學(xué)》2017年碩士論文

【摘要】：芽囊原蟲(Blastocystis sp.)是一種廣泛分布的寄生于腸道的原蟲,在欠發(fā)達(dá)地區(qū)的更為流行,引起腹痛腹瀉等消化系統(tǒng)癥狀,對人的健康和動物的生長發(fā)育具有不良影響。芽囊原蟲的宿主廣泛,可感染人和其他哺乳動物及鳥類。近期的研究發(fā)現(xiàn),珍稀野生動物在人和動物的芽囊原蟲感染過程中具有公共衛(wèi)生隱患。因此,掌握芽囊原蟲的感染情況和種群結(jié)構(gòu)對評估其人獸共患潛能、防控宿主及其具有重要意義。秦嶺地區(qū)珍稀野生動物資源獨(dú)特而豐富,本研究通過基于SSUrRNA基因序列的分子檢測對該地區(qū)兩個野生動物飼養(yǎng)點(diǎn)部分珍稀野生動物進(jìn)行芽囊原蟲感染情況進(jìn)行調(diào)查,并對獲得的陽性分離株進(jìn)行種群結(jié)構(gòu)分析。獲得以下結(jié)果:1.2015年6月至2016年1月,對秦嶺地區(qū)37個物種共497份糞便樣品進(jìn)行芽囊原蟲分子檢測,陽性樣品PCR擴(kuò)增結(jié)果為1,100bp的基因片段。7個動物類群總感染率為40.2%(200/497),其中非人靈長類(75.9%)和有袋類(72.7%)感染率最高,偶蹄類(56.3%)和單蹄類(22.2%)次之,鳥類感染率(2.2%)最低。象類和食肉類為芽囊原蟲陰性。世界范圍內(nèi)首次在果下馬、蒙古野驢、羚牛、羊駝、大羚羊、梅花鹿、馬鹿、列氏水羚、牦牛和白唇鹿中發(fā)現(xiàn)芽囊原蟲。兩個采樣點(diǎn)感染情況一致,均有季節(jié)性變化——夏季到冬季感染率逐漸下降。2.基于芽囊原蟲陽性樣品的SSUrRNA基因的種系進(jìn)化關(guān)系分析,本研究共發(fā)現(xiàn)了13種亞型,包括8種已有亞型(亞型1~3,5,10和12~14)和5種可能的新亞型(暫時命名為亞型18~22)。在已有亞型中,亞型13和5具有人獸共患潛能;可能的新亞型中,亞型19和20可能具有人獸共患潛能。亞型10是優(yōu)勢亞型(38.5%),分布于17種野生動物,亞型5首次在鴕鳥內(nèi)發(fā)現(xiàn)。
[Abstract]:Blastocystis sp. It is a widely distributed protozoa, which is more prevalent in underdeveloped areas, causing abdominal pain, diarrhea and other digestive system symptoms, which has a negative impact on human health and the growth and development of animals. The protozoa has a wide range of hosts and can infect humans and other mammals and birds. Recent studies have found that rare wild animals have a public health hazard in the process of infection of human and animal bud sac protozoa. Therefore, it is important to know the infection status and population structure of Bud cyst protozoa in order to evaluate its zoonotic potential and control its host. Rare and rare wildlife resources in Qinling region are unique and abundant. In this study, the infection of bud cyst protozoa of some rare wild animals in two wild animal breeding sites in this area was investigated by molecular detection based on SSUrRNA gene sequence. The population structure of the positive isolates was analyzed. The following results were obtained: 1. From June 2015 to January 2016, 497 fecal samples from 37 species in Qinling region were detected for protozoa sprouting. The total infection rate of the seven animal groups was 40.22 / 497, including 75.9% of the non-human primate and 72.7% of the marsupial.) the infection rate was the highest, followed by 56.3) and 22.2b), and the infection rate of birds was the lowest. The herbivorous meat was negative for Bud cyst protozoa. Bud sac protozoa was first found in fruit dismount, Mongolian wild donkey, antelope, alpaca, large antelope, sika deer, Wapiti, Lees gazelle, yak and white-lipped deer in the world. The infection rate in the two sampling sites was the same, and there was seasonal variation-the infection rate decreased gradually from summer to winter. Based on phylogenetic analysis of SSUrRNA gene in protozoa positive samples, a total of 13 subtypes were identified in this study, including 8 existing subtypes (subtypes 1, 3, 5, 10 and 12, 14) and 5 possible new subtypes (temporarily named as subtype 18t22). Among the existing subtypes, subtypes 13 and 5 have zoonotic potential, and subtypes 19 and 20 may have zoonotic potential among the possible new subtypes. Subtype 10 is a dominant subtype 38.5, distributed in 17 species of wild animals. Subtype 5 was first found in ostrich.
【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S858.9

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