本文選題：甲烷抑制劑 + 瘤胃發(fā)酵�。� 參考：《黑龍江八一農(nóng)墾大學(xué)》2015年碩士論文

【摘要】：為探究甲烷抑制劑對(duì)綿羊瘤胃發(fā)酵及營(yíng)養(yǎng)物質(zhì)代謝的影響,本試驗(yàn)首先選取6只體況良好,體重相近(28±1.0kg)綿羯羊,安裝永久性瘤胃瘺管,作為瘤胃液供體羊進(jìn)行不同甲烷抑制劑添加劑量的體外篩選試驗(yàn)。在此基礎(chǔ)上選取16只體況良好、體重相近、安裝永久性瘤胃瘺管的綿羊進(jìn)行代謝試驗(yàn),探究甲烷抑制劑對(duì)綿羊瘤胃發(fā)酵及營(yíng)養(yǎng)物質(zhì)代謝的影響。本試驗(yàn)共分兩個(gè)部分。第一部分,選擇6只體況良好、體重相近(28±1.0kg)、安裝永久性瘤胃瘺管的綿羯羊作為瘤胃液供體羊。選取三種甲烷抑制劑且每種抑制劑有三個(gè)水平,分別為每千克飼料中添加延胡索酸(30mg、40mg、50mg)、大蒜素100mg、300mg、500mg)、蒽醌(10 mg、20 mg、100mg),分別在體外培養(yǎng)3、6、9、12和24h,測(cè)定體外發(fā)酵指標(biāo),并篩選出每種甲烷抑制劑的一個(gè)最佳添加水平。第二部分,采取隨機(jī)區(qū)組試驗(yàn)設(shè)計(jì),選擇16只安裝永久性瘤胃瘺管的綿羊,隨機(jī)分為4組,每組4只,其中一組為對(duì)照組飼喂基礎(chǔ)日糧,其余三個(gè)試驗(yàn)組分別在基礎(chǔ)日糧中添加40mg延胡索酸、300mg大蒜素和20mg蒽醌。預(yù)飼期為2周,正試期3天,測(cè)定綿羊瘤胃液pH、氨態(tài)氮、BCP、VFA等發(fā)酵指標(biāo)及甲烷濃度和綿羊?qū)I(yíng)養(yǎng)物質(zhì)代謝指標(biāo)的影響。研究結(jié)果如下:(1)飼料中添加不同劑量延胡索酸、蒽醌、大蒜素對(duì)體外發(fā)酵pH值無(wú)顯著影響(p0.05);飼料中分別添加延胡索酸40mg、大蒜素300mg和蒽醌20mg,顯著提高了體外培養(yǎng)液中BCP(p0.05)、氨態(tài)氮(p0.05)和丙酸(p0.05)濃度,顯著降低了乙酸濃度(p0.05)和乙酸/丙酸(p0.05);顯著提高了12h和24h的總產(chǎn)氣量(p0.05),并對(duì)甲烷產(chǎn)量具有顯著的抑制作用(p0.05)。(2)分別在每千克基礎(chǔ)日糧中添加40mg延胡索酸、300mg大蒜素和20mg蒽醌三種不同甲烷抑制劑,與對(duì)照組相比,對(duì)綿羊瘤胃內(nèi)飼喂后9h內(nèi)的pH值沒(méi)有顯著影響(p0.05),12h時(shí)大蒜素組顯著提高了pH值(p0.05);3個(gè)試驗(yàn)組顯著降低了6h后瘤胃內(nèi)氨態(tài)氮濃度(p0.05),并顯著提高了9h后的瘤胃BCP濃度,其中以大蒜素組最高;有降低乙酸、乙酸/丙酸比及提高丙酸和總VFA的趨勢(shì)(p0.05)。對(duì)瘤胃內(nèi)甲烷產(chǎn)量有顯著影響(p0.05),添加大蒜素和蒽醌6h后甲烷濃度分別比對(duì)照組降低38.28%和37.61%。(3)與對(duì)照組相比日糧中添加蒽醌和大蒜素顯著提高了能量代謝率(p0.05),其中以大蒜素組最高;顯著提高了DM、OM、CP、NDF、ADF、Ca和P的表觀消化率(p0.05),其中以大蒜素組最高;日糧中添加大蒜素顯著提高了干物質(zhì)采食量和可消化氮量(p0.05)。本試驗(yàn)表明,綿羊日糧中添加300mg大蒜素有利于促進(jìn)瘤胃發(fā)酵,降低甲烷產(chǎn)量和提高營(yíng)養(yǎng)物質(zhì)代謝率的作用。
[Abstract]:In order to investigate the effect of methane inhibitor on rumen fermentation and nutrient metabolism of sheep, this experiment first selected 6 good body conditions and similar body weight (28 + 1.0kg) of sheep. The permanent rumen fistula was installed as the donor sheep of the tumor gastric juice in vitro screening test with different dosage of methane inhibitor. On this basis, 16 bodies were selected. The effects of methane inhibitor on rumen fermentation and nutrient metabolism of sheep were investigated with similar weight, and the effects of methane inhibitors on rumen fermentation and nutrient metabolism were investigated. The test was divided into two parts. The first part, the selection of 6 bodies was good, and the body weight was similar (28 + 1.0kg). The selection of permanent rumen fistula was selected as the donor sheep of the tumor gastric juice. Three kinds of methane inhibitors and three levels of each inhibitor were added to each kilogram of 30mg, 40mg, 50mg, allicin 100mg, 300mg, 500mg), anthraquinone (10 mg, 20 mg, 100mg) in vitro culture 3,6,9,12 and 24h, and the optimum addition level of each type of methane inhibitor was screened, and the optimum addition level of each methane inhibitor was screened. Second 16 sheep with permanent rumen fistula were selected and divided into 4 groups randomly, with 4 rats in each group. One group was fed with basal diet, and the other three groups added 40mg Corydalis, 300mg allicin and 20mg anthraquinone in the basal diet. The pre feeding period was 2 weeks, and the sheep tumor was measured for 3 days. The sheep tumor was measured. The effects of pH, ammonia nitrogen, BCP, VFA and the concentration of methane and sheep on the metabolic indices of nutrients were observed. The results were as follows: (1) the addition of different doses of corydal acid, anthraquinone and Allicin had no significant effect on the pH value in vitro (P0.05). The dietary supplemented tetrahydropalmatic acid 40mg, allicin 300mg and anthraquinone 20mg, respectively. The concentration of BCP (P0.05), ammonia nitrogen (P0.05) and propionic acid (P0.05) in the extracorporeal culture significantly reduced the concentration of acetic acid (P0.05) and acetic acid / propionic acid (P0.05), significantly increased the total gas production (P0.05) of 12h and 24h (P0.05), and had a significant inhibitory effect on methane production (P0.05). (2) the 40mg fumaric acid was added to the basal diet of each kilogram, and 300mg was larger. The three different methane inhibitors of allicin and 20mg anthraquinone had no significant effect on the pH value in 9h after rumen feeding in the sheep (P0.05), and the allicin group significantly increased the pH value (P0.05) at 12h, and the 3 experimental groups significantly reduced the concentration of ammonia nitrogen in the rumen after 6h (P0.05), and significantly increased the BCP concentration of the rumen after 9h. The trend of reducing acetic acid, acetic acid / propionic acid ratio and increasing propionic acid and total VFA (P0.05) had a significant effect on the methane production in the rumen (P0.05). The methane concentration of allicin and anthraquinone 6h was reduced by 38.28% and 37.61%. (3), respectively, compared with the control group, and the energy metabolism was significantly increased with the addition of anthraquinone and Allicin in the diet. Rate (P0.05), which was the highest in allicin group, significantly increased the apparent digestibility (P0.05) of DM, OM, CP, NDF, ADF, Ca and P, which was the highest in the allicin group; the addition of allicin in the diet significantly increased the intake of dry matter and the amount of digestible nitrogen (P0.05). The experiment showed that the addition of 300mg allicin in the diet of sheep was beneficial to the rumen fermentation. The role of methane production and nutrient metabolic rate increase.

【學(xué)位授予單位】：黑龍江八一農(nóng)墾大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S826

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