本文選題：灘羊 + 皮膚組織�。� 參考：《寧夏大學(xué)》2017年碩士論文

【摘要】：灘羊是我國唯一生產(chǎn)名貴二毛裘皮的特有綿羊品種,因其特殊地理生態(tài)環(huán)境的長(zhǎng)期自然選擇和人工選育,造就了寧夏灘羊集產(chǎn)肉、產(chǎn)毛、產(chǎn)皮為一體的名貴羊種。灘羊裘皮以美觀、輕便、保暖、耐用而聞名,其在二毛期由于毛股花形不同,因而表現(xiàn)出不同的花穗類型,但隨著二毛期灘羊的日齡增加,其裘皮毛股彎曲等特征會(huì)逐漸減弱直至消失。灘羊皮膚作為這些特征的承載者,通過對(duì)皮膚蛋白質(zhì)組的研究,從而了解其內(nèi)在原因。基于上述背景,本研究開展以下方面的工作:利用雙向凝膠電泳(2-DE)技術(shù)研究一周歲內(nèi)灘羊皮膚差異表達(dá)蛋白,對(duì)一周歲內(nèi)4個(gè)生長(zhǎng)時(shí)期的灘羊皮膚蛋白圖譜進(jìn)行兩兩之間進(jìn)行比對(duì),共發(fā)現(xiàn)了 19個(gè)差異蛋白點(diǎn),質(zhì)譜成功鑒定出15個(gè)蛋白點(diǎn)共13種蛋白。可能與灘羊皮膚生長(zhǎng)調(diào)控有關(guān)的蛋白有14-3-3蛋白σ亞型、膜聯(lián)蛋白A2、角蛋白25、微管蛋白α鏈。對(duì)差異表達(dá)蛋白進(jìn)行分析,發(fā)現(xiàn)這些差異表達(dá)蛋白可能與灘羊皮膚不同生長(zhǎng)階段的毛囊呈周期性變化有關(guān)。應(yīng)用同位素標(biāo)記相對(duì)和絕對(duì)定量(iTRAQ)蛋白質(zhì)組學(xué)方法,聯(lián)合多維液相色譜串聯(lián)質(zhì)譜(LC-MS/MS)技術(shù)對(duì)灘羊、內(nèi)蒙古蘇尼特、中衛(wèi)山羊和新吉細(xì)毛羊的羊皮膚蛋白進(jìn)行差異表達(dá)研究,并應(yīng)用Proteome Discoverer 1.4軟件進(jìn)行定量分析,結(jié)合數(shù)據(jù)庫搜索,鑒定出差異蛋白;最后對(duì)差異蛋白進(jìn)行GO和Pathway分析。四種羊皮膚中共鑒定到1161個(gè)蛋白,其中內(nèi)蒙古蘇尼特羊與灘羊比對(duì)中發(fā)現(xiàn)30個(gè)差異蛋白,中衛(wèi)山羊與灘羊比對(duì)發(fā)現(xiàn)112個(gè)差異蛋白,新吉細(xì)毛羊比對(duì)與灘羊發(fā)現(xiàn)107個(gè)差異蛋白,對(duì)各比對(duì)組的皮膚差異蛋白進(jìn)行分析,發(fā)現(xiàn)發(fā)現(xiàn)KRTAP6、KRTAP11.1、KRT25、KRT34、KRT83和TCHH可能與灘羊羊毛粗細(xì)及彎曲度形成相關(guān)。應(yīng)用iTRAQ蛋白質(zhì)組學(xué)方法,聯(lián)合LC-MS/MS技術(shù)對(duì)灘羊二毛期串子花型、軟大花型、綠豆絲型和其它不規(guī)則型4種花穗型裘皮蛋白進(jìn)行差異表達(dá)研究,在4種花穗型裘皮共鑒定得2886個(gè)蛋白,軟大花型與串子花型比對(duì)獲得差異蛋白數(shù)為135個(gè);綠豆絲型與串子花型比對(duì)獲得差異蛋白142個(gè);不規(guī)則型與串子花型比對(duì)獲得差異蛋白為113個(gè),對(duì)表達(dá)差異蛋白進(jìn)行分析,發(fā)現(xiàn)膜聯(lián)蛋白與VEGF信號(hào)通路與軟大花型毛股形成相關(guān),KRTAP3和KRTAP6可能與灘羊串子花型毛股結(jié)構(gòu)相關(guān)。上述研究為灘羊皮膚的年變化規(guī)律和優(yōu)質(zhì)二毛裘皮選育以及灘羊串子花型二毛裘皮育種提供理論基礎(chǔ)和科學(xué)依據(jù)。
[Abstract]:Tan sheep is the only unique sheep breed producing rare and rare fur in China. Due to the long-term natural selection and artificial breeding of its special geographical and ecological environment, Tan sheep in Ningxia is a rare sheep that produces meat, wool and skin. Tan Sheep fur is famous for its beauty, lightness, warmth, durability and durability. It shows different flower spike types in the period of 20 wool because of the different flower shape, but with the increase of the age of Tan sheep in the period of 20 wool, Its fur hair and other characteristics such as thigh bending will gradually weaken until disappear. As the carrier of these characteristics, the skin of Tan Sheep is studied on the proteome of skin to understand its internal causes. Based on the above background, this study carried out the following work: Two-Dimensional Gel Electrophoresis (2-DEE) technique was used to study the differentially expressed proteins in the skin of Tan Sheep within one year old. A total of 19 differential protein spots were found in the skin protein maps of Tan sheep in four growing stages within one year of age, and 13 proteins were successfully identified by mass spectrometry. There are 14-3-3 protein 蟽 subtypes, annexin A2, keratin 25 and tubulin 偽 chain, which may be related to the regulation of skin growth in Tan sheep. It was found that these differentially expressed proteins might be related to the periodic changes of hair follicles in different growth stages of Tan sheep skin. The differential expression of sheep skin protein in Tan sheep, Inner Mongolia Sunita, Zhongwei goat and Xinji fine wool sheep was studied by using isotopic labeling relative and absolute quantitative ITRAQ proteomics combined with Multidimensional liquid Chromatography tandem Mass Spectrometry (MLDMS) technique in Tan sheep, Inner Mongolia Sunita, Zhongwei goat and Xinji fine wool sheep. The differential protein was identified by using Proteome Discoverer 1.4 software and database search. Finally, the differential protein was analyzed by go and Pathway. A total of 1,161 proteins were identified in the skin of four kinds of sheep, of which 30 were found in the comparison of Sunita sheep and Tan sheep in Inner Mongolia, 112 differential proteins in Zhongwei goat and Tan sheep, 107 differential proteins in Xinji fine wool sheep and Tan sheep. By analyzing the skin differential proteins of the control groups, it was found that KRTAP6, KRTAP11.1, KRT25, KRT34, KRT83 and TCHH might be related to the formation of wool thickness and curvature of Tan sheep. ITRAQ proteomics and LC-MS/MS technique were used to study the differential expression of Fur skin proteins in four flower spike types of Tan Sheep, such as flower pattern, soft large flower type, mung bean silk type and other irregular type. A total of 2886 proteins were identified in four flower panicle types of fur. The number of differentially expressed proteins was 135 in the ratio of soft large flower type to string flower type, 142 in mung bean silk and string flower type. 113 differentially expressed proteins were obtained by comparison of irregular and string flower types. It was found that VEGF signal pathway and VEGF signal pathway were related to the formation of soft large floral hair strands. KRTAP3 and KRTAP6 might be related to the floral pattern of hair strands of Tan sheep. The above studies provide theoretical and scientific basis for the annual variation of Tan sheep skin and the breeding of fine quality Ermao fur and the breeding of Tan sheep's flower pattern of Ermao fur.
【學(xué)位授予單位】：寧夏大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S826

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