本文選題：禽白血病病毒 + 泄殖腔棉拭子��； 參考：《中國獸醫(yī)學(xué)報》2017年04期

【摘要】：為探討泄殖腔棉拭子或種蛋中禽白血病病毒(ALV)-p27抗原檢測與病毒分離檢測之間的相關(guān)性,對不同類型雞編號后分別對應(yīng)采集泄殖腔棉拭子或蛋清后以ALV-p27抗原檢測試劑盒檢測,同時無菌采集抗凝血接種DF-1細胞進行ALV的分離鑒定。結(jié)果顯示,人工感染A亞群ALV的SPF雞群泄殖腔棉拭子ALV-p27抗原檢測與病毒分離有很高的吻合率,相對于病毒分離法,119次泄殖腔棉拭子ALV-p27抗原檢測中出現(xiàn)有1例假陽性和3例假陰性;從美國進口的200只祖代肉雞3次采血DF1細胞分離病毒結(jié)果均為陰性,而泄殖腔棉拭子ALV-p27抗原陽性率依次為4.00%(8/200),2.97%(5/168)和2.61%(4/153),假陽性率為3.30%。相對于病毒分離法,正在實施ALV凈化的不同遺傳背景地方品系雞泄殖腔棉拭子ALV-p27抗原檢測的假陽性率高達93.20%~100.00%,還有一定比例假陰性(1/9,1/4,1/3)。本研究大量對應(yīng)比較的數(shù)據(jù)表明,相對于血漿病毒分離法,不同類型雞的泄殖腔棉拭子ALVp27抗原檢測法不僅有很高的假陽性率,還有一定比例的漏檢率。在種雞場的ALV凈化過程中考慮到成本,不建議以泄殖腔棉拭子作為檢測材料。本試驗為我國雞群禽白血病凈化檢測和臨床鑒別診斷提供參考依據(jù)。
[Abstract]:In order to investigate the correlation between the detection of ALVS-p27 antigen of avian leukemia virus in cloacal cotton swabs or eggs and the detection of virus isolation, the ALV-p27 antigen detection kit was used to detect the number of different types of chicken corresponding to the collection of cloacal cotton swabs or egg whites. At the same time, anticoagulant blood samples were collected and inoculated with DF-1 cells for isolation and identification of ALV. The results showed that the detection of ALV-p27 antigen in cloacal cotton swabs of SPF chickens infected artificially with subgroup A ALV had a high coincidence rate with virus isolation. There were 1 false positive and 3 false negative in ALV-p27 antigen detection of cloacal cotton swabs compared with virus isolation method, and the results of virus isolation from the blood DF1 cells of 200 broilers imported from the United States were all negative. The positive rates of ALV-p27 antigen in cloacal cotton swabs were 4.00 / 200 / 2.97 and 2.61 / 153, respectively. The false positive rate was 3.30%. Compared with virus isolation method, the false positive rate of ALV-p27 antigen detection in cloacal cotton swab of local strain chicken is 93.20 ~ 100.00and a certain proportion of false negative rate is 9 / 9 / 41 / 1 / 3 / 3. Compared with virus isolation method, local chicken cloacal cotton swab from different genetic background is carrying out ALV purification, and the false positive rate of ALV-p27 antigen is as high as 93.20%. A large number of corresponding data showed that compared with plasma virus isolation method, ALVp27 antigen detection of cloacal cotton swabs of different types of chickens not only has a high false positive rate, but also has a certain percentage of missed detection rate. The cloacal cotton swab is not recommended to be used as the detection material in the process of ALV purification in chicken farm. This study provides reference for purification detection and clinical differential diagnosis of avian leukemia in China.
【作者單位】： 山東農(nóng)業(yè)大學(xué)動物科技學(xué)院;中國農(nóng)業(yè)科學(xué)院家禽研究所;
【基金】：國家公益性行業(yè)(農(nóng)業(yè))科研專項種雞場禽白血病防控與凈化技術(shù)的集成資助項目(201203055)
【分類號】：S858.31
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本文編號：1831578