發(fā)布時間：2018-04-30 19:02

  本文選題：種公羊 + 血清生化�。� 參考：《西北農(nóng)林科技大學》2015年碩士論文

【摘要】：本試驗通過飼養(yǎng)試驗和消化試驗,研究日糧蛋白質(zhì)水平對絨山羊種公羊生產(chǎn)性能、血液指標和精液品質(zhì)的影響,綜合考慮確定種公羊的蛋白需要量,為建立我國絨山羊營養(yǎng)需要模型奠定基礎(chǔ),從而為最大限度的提高我國絨山羊生產(chǎn)性能以及飼料利用效率提供理論和應用基礎(chǔ)。試驗一:日糧蛋白水平對陜北白絨山羊種公羊體重和養(yǎng)分消化率的影響試驗選取12只陜北白絨山羊種公羊,采用隨機區(qū)組設(shè)計分為4組,每組3只,根據(jù)NRC推薦量的70%,85%,100%,115%分別飼喂4種蛋白水平日糧:5.10%,7.11%,8.07%,9.35%。試驗分為3個時期:配種預備期(50 d)、配種正式期(采精頻率五天兩次期,20 d;五天四次期,20 d),恢復期(30 d)。每個階段均進行消化試驗,測定種公羊的養(yǎng)分消化率。結(jié)果發(fā)現(xiàn):(1)各時期不同處理組種公羊日增重差異顯著(P0.05),日增重隨蛋白水平的升高而升高。(2)各時期不同處理組種公羊的粗蛋白消化率差異顯著(P0.05),但干物質(zhì)、粗纖維、中性洗滌纖維和酸性洗滌纖維消化率均差異不顯著(P0.05)。試驗二:日糧蛋白水平對陜北白絨山羊種公羊血清生化指標、生殖激素含量和精液品質(zhì)的影響試驗選取12只陜北白絨山羊種公羊,采用隨機區(qū)組設(shè)計分為4組,每組3只,分別飼喂4種蛋白水平日糧:5.10%,7.11%,8.07%,9.35%。試驗分為3個時期:配種預備期(50 d)、配種正式期(采精頻率五天兩次期,20 d;五天四次期,20 d),恢復期(30 d)。試驗使用假陰道法采集精液,并在每個階段結(jié)束后頸靜脈采血測定血清生化指標和生殖激素含量。結(jié)果發(fā)現(xiàn):(1)日糧蛋白水平對預備期種公羊血清中GPT和GOT活性,TP、ALB、GLO、A/G和GLU的含量無顯著影響(P0.05),而對BUN影響顯著(P0.05),BUN隨日糧蛋白水平升高而升高。(2)日糧蛋白水平對種公羊FSH、LT和T的濃度無顯著影響(P0.05),但配種階段對種公羊FSH和睪酮含量有顯著影響(P0.05)。(3)日糧蛋白水平對種公羊射精量、密度和有效精子數(shù)有顯著影響,均隨日糧蛋白水平的升高而升高。日糧蛋白水平對精子活力無顯著影響,但隨蛋白水平的升高有上升趨勢(P0.05)。試驗三:陜北白絨山羊種公羊蛋白需要量研究試驗采用回歸法和析因法相結(jié)合的方法,研究陜北白絨山羊種公羊在各配種階段的蛋白需要量,并結(jié)合試驗結(jié)果,綜合考慮確定種公羊的蛋白需要量。獲得陜北白絨山羊種公羊在各時期的粗蛋白(CPI)和可消化粗蛋白(DCPI)需要量回歸方程為:預備期:CPI 5.095 W.0.844ADG(R2=0.997,P=0.001)DCPI 2.296 W.0.772ADG(R2=0.978,P=0.007)配種正式期:CPI 5.095W.44.20n(n,采精次數(shù))DCPI 2.296W.38.15n (n,采精次數(shù))恢復期:CPI 4.312 W.0.841ADG(R2=0.977,P=0.008)DCPI 2.215 W.0.803ADG(R2=0.963,P=0.012)綜上所述,日糧蛋白水平對種公羊日增重、粗蛋白消化率、血清尿素氮、射精量、密度和有效精子數(shù)有顯著影響,這些指標均隨蛋白水平升高而升高。
[Abstract]:The effects of dietary protein level on production performance, blood index and semen quality of Cashmere Goats were studied by feeding and digesting experiments. In order to establish the nutritional requirement model of Chinese Cashmere Goats (Cashmere Goats) and provide the theoretical and practical basis for the maximum improvement of the production performance and feed utilization efficiency of Chinese Cashmere Goats (Cashmere Goats). Experiment 1: effects of dietary protein levels on body weight and nutrient digestibility of white cashmere goat breeds in Northern Shaanxi; 12 white cashmere goats were divided into 4 groups with 3 in each group. According to the recommended amount of NRC, 70%, 85% and 100% were fed with 4 kinds of protein levels, respectively, and 7. 11% and 8. 07%, 9. 35%, respectively. The experiment was divided into three periods: breeding preparation period (50 days), formal breeding period (spermatozoa frequency: five days, two periods, 20 days, five days, four periods, 20 days, convalescence, 30 days). The nutrient digestibility of male sheep was determined by digestion test in each stage. The results showed that there were significant differences in daily gain (ADG) between different treatments (P 0.05), and daily gain (ADG) increased with the increase of protein level. (2) the digestibility of crude protein in different treatments was significantly different (P 0.05), but dry matter, crude fiber, dry matter and crude fiber were significantly different between different treatments. There was no significant difference in digestibility of neutral detergent fiber and acid detergent fiber. Experiment 2: effects of dietary protein level on serum biochemical index, reproductive hormone content and semen quality of white cashmere goat breeds in Northern Shaanxi, 12 white cashmere goats were divided into 4 groups with 3 in each group. Each of them was fed with 4 kinds of protein levels: 7.11% and 8.07% and 9.35%, respectively. The experiment was divided into three periods: breeding preparation period (50 days), formal breeding period (spermatozoa frequency: five days, two periods, 20 days, five days, four periods, 20 days, convalescence, 30 days). Semen was collected by pseudovaginal method and blood samples were collected from jugular vein at the end of each stage to determine serum biochemical indexes and reproductive hormone content. The results showed that dietary protein level had no significant effect on the contents of GPT and GOT activity in serum of male sheep during the preparation period, but had no significant effect on the content of P0.05G and GLU in serum of male sheep. However, it had a significant effect on BUN. The dietary protein level increased with the increase of dietary protein level, and the effect of dietary protein level on FSHLT of male sheep was higher than that of dietary protein level. And the concentration of T had no significant effect on the ejaculation of male sheep, but the content of FSH and testosterone in breeding ram was significantly affected by dietary protein level. The density and the number of effective sperm increased with the increase of dietary protein level. Dietary protein level had no significant effect on sperm motility, but increased with the increase of protein level. Experiment 3: protein requirement of White Cashmere Goat breeds in Northern Shaanxi Province combined with regression method and factorization method, the protein requirement of white cashmere goat in different breeding stages was studied, and the results of the experiment were combined. To determine the protein requirement of male sheep. 鑾峰緱闄曞寳鐧界粧灞辯緤縐嶅叕緹婂湪鍚勬椂鏈熺殑綺楄泲鐧，

本文編號：1825787