本文選題：殼聚糖 + 蛋種雞　； 參考：《內(nèi)蒙古農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：本論文共分3個(gè)試驗(yàn),研究了殼聚糖對(duì)蛋種雞抗氧化功能的影響及其作用機(jī)制。采用單因子完全隨機(jī)試驗(yàn)設(shè)計(jì),將450只產(chǎn)蛋率(92.44%± 0.030)26周齡海蘭褐殼種用蛋雞隨機(jī)分為1個(gè)對(duì)照組和4個(gè)殼聚糖處理組,每組6個(gè)重復(fù),每個(gè)重復(fù)15只雞。對(duì)照組飼喂基礎(chǔ)日糧,不添加殼聚糖,殼聚糖處理組分別在基礎(chǔ)日糧中添加250、500、1000、2000 mg/kg的殼聚糖。預(yù)試期1周,正試期8周。試驗(yàn)1主要研究殼聚糖對(duì)蛋種雞血清、肝臟和十二指腸組織中抗氧化指標(biāo)的影響;試驗(yàn)2主要通過(guò)研究殼聚糖對(duì)蛋種雞肝臟和十二指腸組織ARA代謝與MAPK信號(hào)通路的影響,探討殼聚糖對(duì)蛋種雞抗氧化功能的作用機(jī)制。試驗(yàn)3主要通過(guò)研究殼聚糖對(duì)蛋種雞肝臟和十二指腸組織抗氧化酶相關(guān)基因及Nrf2基因表達(dá)量的影響,從轉(zhuǎn)錄水平上進(jìn)一步探討殼聚糖對(duì)蛋種雞抗氧化功能影響的分子機(jī)理。在本研究條件下,初步得出以下結(jié)論:①日糧添加殼聚糖對(duì)蛋種雞血清、肝臟和十二指腸組織中GSH-Px、T-SOD與TrxR活性和抗超氧陰離子能力的影響均存在二次曲線劑量依賴關(guān)系。日糧添加250～1000mg/kg的殼聚糖對(duì)蛋種雞血清、肝臟和十二指腸組織中抗氧化功能的促進(jìn)效果均較好,2000 mg/kg的殼聚糖促進(jìn)作用減弱,甚至?xí)霈F(xiàn)抑制作用。②在試驗(yàn)第8周,隨著日糧殼聚糖添加劑量的增加,蛋種雞肝臟和十二指腸組織JNK與ERK1/2活性呈一次線性升高,而十二指腸組織ARA與LTB4含量、cPLA2活性呈二次曲線升高;并以250～500 mg/kg殼聚糖組的促進(jìn)作用較強(qiáng);當(dāng)2000 mg/kg水平時(shí)促進(jìn)作用減弱。③日糧添加殼聚糖以二次曲線升高模式影響蛋種雞肝臟和十二指腸組織中GSH-Px、SOD1、SOD2、TrxR1抗氧化酶基因和Nrf2基因表達(dá)量;其中,以250～500 mg/kg殼聚糖組較高,2000 mg/kg殼聚糖組則有降低趨勢(shì)。④殼聚糖對(duì)蛋種雞抗氧化功能影響的可能機(jī)制是:殼聚糖通過(guò)上調(diào)Nrf2基因表達(dá),激活Nrf2信號(hào)通路,進(jìn)而調(diào)控下游抗氧化酶的基因表達(dá),增強(qiáng)抗氧化酶活性,促進(jìn)蛋種雞的抗氧化功能;殼聚糖通過(guò)MAPK信號(hào)通路調(diào)節(jié)十二指腸組織中ARA的釋放,減緩機(jī)體的氧化應(yīng)激,保持動(dòng)物機(jī)體良好的代謝狀態(tài)。⑤綜合各項(xiàng)指標(biāo)得出,蛋種雞日糧殼聚糖的添加劑量為250～500 mg/kg時(shí)具有較強(qiáng)的抗氧化功能。
[Abstract]:In this paper, three experiments were conducted to study the effect of chitosan on antioxidant function and its mechanism of egg breeding chicken. Using a single factor complete random trial design, 450 Hailan brown shell hens at the age of 92.44% 鹵0.030 ~ 26 weeks were randomly divided into one control group and four chitosan treatment groups, 6 replicates in each group and 15 chickens in each replica. The control group was fed the basal diet without chitosan. The chitosan treatment group was fed with the basic diet of 250500 ~ 1000 ~ 2 000 mg/kg. The pretest period was 1 week and the positive test period was 8 weeks. In experiment 1, the effects of chitosan on antioxidant indexes in serum, liver and duodenum were studied, and in experiment 2, the effects of chitosan on ARA metabolism and MAPK signaling pathway in liver and duodenum were studied. To explore the mechanism of chitosan on antioxidant function of laying chicken. In experiment 3, the effects of chitosan on the expression of antioxidant enzyme related genes and Nrf2 genes in liver and duodenum of laying hens were studied, and the molecular mechanism of the effects of chitosan on antioxidant function of laying chickens was further studied at the transcriptional level. Under the condition of this study, it was concluded that the effect of adding chitosan on the activity of TrxR and the activity of superoxide anion in serum, liver and duodenum of laying chicken were conic dose-dependent. The effect of chitosan supplemented with 250~1000mg/kg on antioxidant function in serum, liver and duodenum of laying chicken was better. The effect of chitosan in 2000 mg/kg was weakened, and even inhibited by 2. 2 in the 8th week of the experiment. The activities of JNK and ERK1/2 in liver and duodenum of laying hens increased linearly with the increase of dietary chitosan additive content, while the activities of ARA and LTB4 in duodenum tissues increased in quadratic curve. At the level of 2000 mg/kg, the effects of supplementation of chitosan on the liver and duodenum of laying chicken were increased by quadratic curve, and the expression of GSH-PxX SOD1SOD2 and TrxR1 antioxidation enzyme gene and Nrf2 gene in the liver and duodenum of the laying chicken were influenced by the supplementation of chitosan at the level of 2000 mg/kg. The possible mechanism of decreasing the effect of chitosan on antioxidant function of laying chickens was that chitosan could activate Nrf2 signaling pathway by up-regulating the expression of Nrf2 gene. Furthermore, it regulates the gene expression of antioxidant enzymes downstream, enhances the activity of antioxidant enzymes, and promotes the antioxidant function of laying chickens. Chitosan regulates the release of ARA from duodenal tissues through MAPK signaling pathway, thus slowing down the oxidative stress of the body. It was concluded that the additive amount of chitosan in the diet of egg fowl had strong antioxidant function when the dosage of chitosan was 250 ~ 500 mg/kg.
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S831.5

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 孫淑豐;叢乃霞;韓剛;;異煙肼作用小鼠肝細(xì)胞Nrf2與SOD及GST表達(dá)的相關(guān)性分析[J];中國(guó)煤炭工業(yè)醫(yī)學(xué)雜志;2016年07期

2 劉梅;鄭美;史挺;;殼聚糖對(duì)果蠅生長(zhǎng)發(fā)育及抗氧化能力的影響[J];食品工業(yè)科技;2015年24期

3 李素娟;金可可;趙亞萍;王鵬;趙文璽;高凱旋;楊文娟;王加林;吳德平;;氧化應(yīng)激激活JNK-MAPK參與波動(dòng)性高血糖誘導(dǎo)的大鼠肝細(xì)胞損傷[J];中國(guó)病理生理雜志;2015年07期

4 李俊良;史彬林;閆素梅;金鹿;徐元慶;李倜宇;郭yN瑋;郭曉宇;;不同殼聚糖濃度培養(yǎng)液對(duì)斷奶仔豬外周血淋巴細(xì)胞中花生四烯酸代謝的影響[J];動(dòng)物營(yíng)養(yǎng)學(xué)報(bào);2014年01期

5 趙英政;徐光翠;吳輝;韓光亮;張合喜;;殼聚糖對(duì)鎘致大鼠肝臟線粒體氧化損傷的影響[J];環(huán)境與健康雜志;2013年10期

6 高忠新;孫淑靜;李振;;殼聚糖硒對(duì)肉仔雞生產(chǎn)性能、腸道菌群的影響[J];糧食與飼料工業(yè);2013年09期

7 趙曉旭;楊佐君;滑靜;王曉霞;張潔;;硒化殼聚糖對(duì)種公雞組織硒含量、硒酶活性及其基因表達(dá)的影響[J];動(dòng)物營(yíng)養(yǎng)學(xué)報(bào);2013年05期

8 方靜;曹聳;李振;;殼聚糖硒對(duì)肉仔雞免疫功能及抗氧化作用的影響[J];糧食與飼料工業(yè);2013年02期

9 陳剛耀;唐志剛;溫超;楊衛(wèi)兵;周巖民;;低聚殼聚糖對(duì)生長(zhǎng)育肥豬抗氧化性能、脂類代謝和肌肉品質(zhì)的影響[J];糧食與飼料工業(yè);2012年08期

10 陶磊;傅淑霞;;花生四烯酸與氧化應(yīng)激的研究進(jìn)展[J];中國(guó)病理生理雜志;2011年11期

相關(guān)會(huì)議論文 前1條

1 喬麗紅;趙穎;唐志剛;溫超;周巖民;;低聚殼聚糖對(duì)斷奶仔豬血清生化指標(biāo)、抗氧化性能和糞便微生物的影響[A];中國(guó)畜牧獸醫(yī)學(xué)會(huì)動(dòng)物營(yíng)養(yǎng)學(xué)分會(huì)第十一次全國(guó)動(dòng)物營(yíng)養(yǎng)學(xué)術(shù)研討會(huì)論文集[C];2012年

相關(guān)博士學(xué)位論文 前4條

1 劉志友;日糧添加殼聚糖對(duì)蛋種雞脂質(zhì)代謝的影響及其機(jī)理研究[D];內(nèi)蒙古農(nóng)業(yè)大學(xué);2016年

2 張瑩;自然放牧與放牧補(bǔ)飼育肥對(duì)呼倫貝爾羔羊與呼杜雜一代羔羊脂肪與蛋白質(zhì)代謝的影響[D];內(nèi)蒙古農(nóng)業(yè)大學(xué);2016年

3 李慧英;殼聚糖對(duì)肉雞免疫功能的影響及其分子機(jī)理研究[D];內(nèi)蒙古農(nóng)業(yè)大學(xué);2009年

4 史彬林;殼聚糖對(duì)肉仔雞生長(zhǎng)性能和免疫功能的影響及其機(jī)理的研究[D];中國(guó)農(nóng)業(yè)大學(xué);2005年

相關(guān)碩士學(xué)位論文 前3條

1 趙啟龍;殼聚糖對(duì)蛋種雞產(chǎn)蛋性能、免疫和抗氧化功能及相關(guān)血清生化指標(biāo)的影響[D];內(nèi)蒙古農(nóng)業(yè)大學(xué);2015年

2 張嚴(yán)偉;低聚殼聚糖對(duì)福瑞鯉生長(zhǎng)、免疫及抗氧化功能的影響[D];南京農(nóng)業(yè)大學(xué);2012年

3 于志軍;殼寡糖通過(guò)核轉(zhuǎn)錄因子-κB誘導(dǎo)巨噬細(xì)胞產(chǎn)生一氧化氮[D];浙江大學(xué);2004年

，

本文編號(hào)：1818808