本文選題：奶牛 + 乳腺上皮細(xì)胞��； 參考：《動(dòng)物營養(yǎng)學(xué)報(bào)》2017年09期

【摘要】：本試驗(yàn)旨在研究賴氨酸(Lys)對奶牛乳腺上皮細(xì)胞(BMECs)內(nèi)乳脂肪合成相關(guān)基因和蛋白表達(dá)的影響,探討Lys影響乳脂肪合成的機(jī)理。將第3代BMECs隨機(jī)分為6組,每組6個(gè)重復(fù),每個(gè)重復(fù)1個(gè)培養(yǎng)孔。各組培養(yǎng)基中Lys的濃度分別為0.5(基礎(chǔ)培養(yǎng)基,對照)、1.0、2.0、4.0、8.0和16.0mmol/L,37℃、5%CO2培養(yǎng)48h后測定BMECs甘油三酯(TAG)含量、乳脂肪合成相關(guān)基因和蛋白的表達(dá)量。結(jié)果表明:BMECs內(nèi)TAG含量(P=0.013)以及脂肪酸結(jié)合蛋白3(FABP3,P=0.001)、脂蛋白脂酶(LPL,P=0.096)、脂肪酸合成酶(FASN,P=0.003)、乙酰甘油磷酸脂酰轉(zhuǎn)移酶6(AGPAT6,P=0.038)和甘油-3-磷酸�；D(zhuǎn)移酶(GPAM,P=0.022)基因表達(dá)量對Lys呈顯著或趨于顯著的濃度依賴效應(yīng)。FABP3基因表達(dá)量以2.0、4.0、8.0、16.0mmol/L組和LPL基因表達(dá)量以1.0、2.0、4.0、8.0、16.0 mmol/L組顯著高于0.5mmol/L組(P0.05);FASN基因表達(dá)量以2.0mmol/L組最高,顯著高于16.0mmol/L組(P0.05);硬脂酰輔酶A去飽和酶1(SCD1)基因表達(dá)量以2.0、4.0mmol/L組顯著高于其他組(P0.05);磷脂酸磷酸酯酶1(LPIN1)、嗜乳脂蛋白亞家族1成員1(BTN1 A1)和黃嘌呤脫氫酶(XDH)基因表達(dá)量均以1.0、2.0、4.0、8.0mmol/L組顯著高于0.5mmol/L組(P0.05);過氧化物酶體增殖物激活受體γ(PPARγ)基因及蛋白表達(dá)量均以2.0、4.0mmol/L組顯著高于0.5和8.0、16.0mmol/L組(P0.05);固醇調(diào)節(jié)元件結(jié)合蛋白1(SREBP1)基因表達(dá)量以1.0、2.0、4.0mmol/L組顯著高于其他組(P0.05),蛋白表達(dá)量以1.0 mmol/L組顯著高于其他組(P0.05)。但高濃度Lys抑制AGPAT6和GPAM的基因表達(dá),AGPAT6基因表達(dá)量以2.0、4.0、8.0、16.0mmol/L組顯著低于0.5、1.0mmol/L組(P0.05),GPAM基因表達(dá)量以16.0mmol/L組顯著低于0.5、1.0、2.0、4.0mmol/L組(P0.05)�？梢�,Lys對BMECs的乳脂肪合成具有顯著的促進(jìn)效果,但高濃度的Lys抑制了乳脂肪合成相關(guān)基因的表達(dá)。本試驗(yàn)條件下,培養(yǎng)基中Lys適宜濃度為2.0~4.0mmol/L。
[Abstract]:The purpose of this experiment was to investigate the effect of lysine (Lys) on the gene and protein expression of milk fat synthesis in dairy cow's mammary epithelial cells (BMECs), and to explore the mechanism of Lys affecting milk fat synthesis. Third generation of BMECs were randomly divided into 6 groups, 6 replicates in each group, and 1 culture holes per repeat. The concentration of Lys in each culture medium was 0.5 (basic medium, The content of BMECs triglyceride (TAG) and the expression of related genes and proteins of milk fat synthesis were measured by 1.0,2.0,4.0,8.0 and 16.0mmol/L, 37 C and 5%CO2 for 48h. The results showed that the content of TAG in BMECs (P=0.013), fatty acid binding protein 3 (FABP3, P=0.001), lipoprotein lipase, fatty acid synthetase, acetyl Gan The expression of oil phospholipid transferase 6 (AGPAT6, P=0.038) and glycerol -3- phosphate acyl transferase (GPAM, P=0.022) gene expressed a significant or significant concentration dependence effect on Lys,.FABP3 gene expression in 2.0,4.0,8.0,16.0mmol/L group and LPL gene expression in 1.0,2.0,4.0,8.0,16.0 mmol/L group was significantly higher than that of 0.5mmol/L group. The expression was highest in group 2.0mmol/L, significantly higher than that in group 16.0mmol/L (P0.05), and the expression of stearyl coenzyme A dehydrogenase 1 (SCD1) gene was significantly higher than that in other groups (P0.05); phosphatidic acid phosphatase 1 (LPIN1), 1 member of the fomiphin subfamily 1 (BTN1 A1) and xanthine dehydrogenase (XDH) gene expression were all 1.0,2.0,4.0,8.0mmol The /L group was significantly higher than the 0.5mmol/L group (P0.05), and the gene and protein expression of peroxisome proliferator activated receptor gamma (PPAR gamma) were significantly higher in 2.0,4.0mmol/L group than in group 0.5 and 8.0,16.0mmol/L (P0.05), and the expression of sterol regulatory element binding protein 1 (SREBP1) gene was significantly higher than other groups (P0.05) in 1.0,2.0,4.0mmol/L group (P0.05). The 1 mmol/L group was significantly higher than the other groups (P0.05), but the high concentration of Lys inhibited the gene expression of AGPAT6 and GPAM, and the expression of AGPAT6 gene in 2.0,4.0,8.0,16.0mmol/L group was significantly lower than that in 0.5,1.0mmol/L group (P0.05). The expression of GPAM gene in 16.0mmol/L group was significantly lower than that in 0.5,1.0,2.0,4.0mmol group. The high concentration of Lys inhibited the expression of genes related to milk fat synthesis. Under the experimental conditions, the suitable concentration of Lys in culture medium was 2.0~4.0mmol/L.

【作者單位】： 內(nèi)蒙古農(nóng)業(yè)大學(xué)動(dòng)物科學(xué)學(xué)院;
【基金】：國家奶業(yè)“973計(jì)劃”項(xiàng)目(2011CB1008003)
【分類號(hào)】：S823.5

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