本文選題：番鴨 + 異常行為��； 參考：《基因組學(xué)與應(yīng)用生物學(xué)》2017年04期

【摘要】：為研究促腎上腺皮質(zhì)激素釋放激素結(jié)合蛋白(corticotropin-releasing hormone binding protein,CRHBP)作為番鴨異常行為研究候選基因的可能性。實(shí)驗(yàn)以半番鴨為實(shí)驗(yàn)材料,設(shè)計(jì)9對(duì)引物對(duì)CRHBP基因內(nèi)含子和外顯子序列進(jìn)行擴(kuò)增,其PCR產(chǎn)物經(jīng)測(cè)序后,利用生物信息學(xué)軟件分析SNPs位點(diǎn)突變前后CRHBP基因及其蛋白結(jié)構(gòu)的變化。結(jié)果表明,在擴(kuò)增的CRHBP基因中篩選出12個(gè)SNPs:Exon3-G~(5805)A、Exon3-C~(5906)T、Exon7-A~(10747)G、Intron3-A~(5984)G、Intron3-T~(6019)G、Intron3-C~(6021)T、Intron3-G~(6035)A、Intron3-G~(6036)A、Intron7-C~(10506)T、Intron7-A~(10613)G、Intron8-G~(12097)A和Intron8-G~(12118)A,其中Exon3-G~(5805)A和Exon7-A~(10747)G為同義突變;Exon3-C~(5906)T是錯(cuò)義突變,導(dǎo)致編碼的纈氨酸(Val)突變?yōu)楫惲涟彼?Ile);Intron3-A~(5984)G、Intron3-T~(6019)G、Intron3-C~(6021)T、Intron3-G~(6035)A、Intron3-G~(6036)A、Intron7-C~(10506)T、Intron7-A~(10613)G、Intron8-G~(12097)A和Intron8-G~(12118)A處于內(nèi)含子區(qū)域,不參與氨基酸編碼。
[Abstract]:In order to study the possibility of corticotropin-releasing hormone binding protein (CRHBP) as a candidate gene for the study of abnormal behavior in Muscovy ducks, the experiment was conducted with a semi muscovy duck as the experimental material. 9 pairs of primers were designed to amplify the intron and exons of the CRHBP gene, and the PCR products were sequenced. The changes of CRHBP gene and its protein structure before and after the mutation of SNPs site were analyzed by bioinformatics software. The results showed that 12 SNPs:Exon3-G~ (5805) A, Exon3-C~ (5906) T, Exon7-A~ (10747) G, Intron3-A~ (5984) G, 6019), 6036 (6036), 6036 (6036), were screened in the amplified CRHBP gene. 10506) T, Intron7-A~ (10613) G, Intron8-G~ (12097) A and Intron8-G~ (12118) A, in which Exon3-G~ (5805) A and Exon7-A~ (10747) G are synonymous mutations; Exon3-C~ (5906) is a missense mutation, resulting in the mutation of the encoded valine to isoleucine; 6019 (6021), 6035. Intron7-C~ (10506) T, Intron7-A~ (10613) G, Intron8-G~ (12097) A and Intron8-G~ (12118) A are in intron regions, and do not participate in amino acid coding.

【作者單位】： 貴州大學(xué)動(dòng)物科學(xué)學(xué)院;高原山地動(dòng)物遺傳育種與繁殖省部共建教育部重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金(31360566) 貴州省教育廳產(chǎn)學(xué)研基地項(xiàng)目(黔教合KY字2013-120號(hào)) 貴州省科技廳農(nóng)業(yè)重大專(zhuān)項(xiàng)[黔科合重大專(zhuān)項(xiàng)字(2012)6004號(hào)]基金共同資助
【分類(lèi)號(hào)】：Q811.4;S834
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本文編號(hào)：1801851