本文選題：檳榔江水牛 + Dmrt基因��； 參考：《云南農(nóng)業(yè)大學學報(自然科學)》2017年04期

【摘要】：【目的】Dmrt7基因是Dmrt基因家族成員之一,影響公畜精子形成。迄今,有關水牛精子發(fā)生的遺傳基礎的研究很少,本研究旨在對檳榔江水牛Dmrt7基因完整CDS區(qū)進行克隆和組織差異表達分析�！痉椒ā坎捎肦T-PCR和功能生物信息學方法�！窘Y(jié)果】檳榔江水牛Dmrt7基因完整編碼區(qū)序列長1 113 bp,編碼370個氨基酸;Dmrt7包含DM和DMRT-like兩個保守結(jié)構域,無信號肽,無跨膜結(jié)構,為核內(nèi)蛋白。同源性分析顯示,水牛與其他�？莆锓NDmrt7氨基酸序列相似度達95%以上。在檢測的10個組織中,Dmrt7基因僅在水牛睪丸組織中表達且高表達。在第6外顯子發(fā)現(xiàn)1個SNP位點(c.665AG),引起了p.Q222R的替換,此位點的替換對Dmrt7蛋白功能無影響�！窘Y(jié)論】有助于了解水牛Dmrt7基因的基本功能。
[Abstract]:[objective] Dmrt7 gene is a member of Dmrt gene family, which affects sperm formation in male animals.To date, little research has been done on the genetic basis of spermatogenesis in buffalo.The purpose of this study was to clone the complete CDS region of the Dmrt7 gene and analyze the tissue differential expression of the complete CDS region of the Dmrt7 gene of the water buffalo. [methods] RT-PCR and functional bioinformatics methods were used. [results] the complete coding region of the Dmrt7 gene of the water buffalo was long.1,113bp, encoding 370 amino acids Dmrt7 containing two conserved domains DM and DMRT-like.No signal peptide, no transmembrane structure, is a nuclear protein.Homology analysis showed that the similarity of Dmrt7 amino acid sequence between buffalo and other bovine species was more than 95%.The Dmrt7 gene was only expressed and overexpressed in the testis of buffalo.A SNP locus named c. 665AGN was found at exon 6, which led to the replacement of p.Q222R, which had no effect on the function of Dmrt7 protein. [conclusion] it is helpful to understand the basic function of buffalo Dmrt7 gene.
【作者單位】： 云南農(nóng)業(yè)大學動物科學技術學院;云南農(nóng)業(yè)職業(yè)技術學院畜牧獸醫(yī)學院;云南農(nóng)業(yè)大學農(nóng)科專業(yè)基礎實驗教學示范中心;騰沖市巴福樂檳榔江水牛良種繁育有限公司;
【基金】：國家自然科學基金項目(31460582,30660024) 云南省應用基礎研究重點項目(2014FA032,2007C0003Z) 云南省應用基礎研究計劃面上項目(2006C0034M,2010ZC243)
【分類號】：S823.83
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本文編號：1764805