短鏈酮酸對(duì)AA肉雞生長(zhǎng)性能和血液指標(biāo)的影響
發(fā)布時(shí)間:2018-04-14 06:28
本文選題:短鏈酮酸 + 生長(zhǎng)性能 ; 參考:《東北農(nóng)業(yè)大學(xué)》2015年碩士論文
【摘要】:短鏈酮酸是一種調(diào)節(jié)機(jī)體物質(zhì)代謝的中間產(chǎn)物,作為一種新型飼料添加劑,具有安全無(wú)殘留的特點(diǎn),引起國(guó)內(nèi)外畜牧工作者的廣泛興趣。短鏈酮酸具有一定的促生長(zhǎng)作用,但因動(dòng)物品種及所處生長(zhǎng)階段的不同,其添加水平不盡相同。在本實(shí)驗(yàn)室前期研究的基礎(chǔ)上,試驗(yàn)選取AA肉雞為試驗(yàn)動(dòng)物,觀察不同水平短鏈酮酸對(duì)AA肉雞生長(zhǎng)性能和血液指標(biāo)的影響,確定其在AA肉雞日糧中的適宜添加水平,為短鏈酮酸在實(shí)際生產(chǎn)上的應(yīng)用提供理論依據(jù)和應(yīng)用參數(shù)。試驗(yàn)選取健康、初始體重?zé)o顯著差異的的1日齡AA肉公雞192只,隨機(jī)分為4個(gè)處理,分別為對(duì)照組、試驗(yàn)Ⅰ組、試驗(yàn)Ⅱ組、試驗(yàn)Ⅲ組,每個(gè)處理4個(gè)重復(fù),每個(gè)重復(fù)12只雞。對(duì)照組飼喂基礎(chǔ)日糧,試驗(yàn)Ⅰ、Ⅱ、Ⅲ組在基礎(chǔ)日糧中分別添加0.375%、0.500%和0.625%短鏈酮酸產(chǎn)品。試驗(yàn)期為42天。試驗(yàn)期間每隔2周對(duì)試驗(yàn)雞進(jìn)行一次空腹稱重,并且以重復(fù)為單位對(duì)余料進(jìn)行回稱,計(jì)算平均日增重、平均日采食量和料重比。在試驗(yàn)第29天,從每個(gè)重復(fù)中選取1只接近平均體重的試驗(yàn)雞,空腹稱重后進(jìn)行頸靜脈采血,分離出血清進(jìn)行血液指標(biāo)的測(cè)定,屠宰后取脾臟、胸腺和法氏囊等器官進(jìn)行稱重。結(jié)果表明:1.不同水平短鏈酮酸對(duì)AA肉雞生長(zhǎng)性能的影響:各試驗(yàn)組肉雞在14日齡、28日齡和42日齡時(shí)的體重均高于對(duì)照組,其中試驗(yàn)Ⅰ、Ⅱ組肉雞體重在28日齡時(shí)達(dá)到顯著水平(P0.05)。在相同日齡,試驗(yàn)Ⅱ組肉雞體重最大,分別比對(duì)照組提高2.46%(P0.05)、3.32%(P0.05)和6.72%(P0.05),后期增重效果更加明顯。在試驗(yàn)的1-14日齡階段,各試驗(yàn)組肉雞平均日增重均高于對(duì)照組,其中試驗(yàn)Ⅱ組達(dá)到顯著水平(P0.05),平均日采食量提高,但差異不顯著(P0.05)。在試驗(yàn)的1-28日齡階段,試驗(yàn)Ⅰ、Ⅱ組肉雞的平均日增重顯著高于對(duì)照組(P0.05),試驗(yàn)Ⅲ組肉雞的平均日增重與對(duì)照組間差異不顯著(P0.05),各組間肉雞平均日采食量無(wú)顯著差異(P0.05)。在試驗(yàn)的1-42日齡階段,與對(duì)照組相比,各試驗(yàn)組肉雞的平均日采食量增加(P0.05),試驗(yàn)Ⅰ、Ⅱ、Ⅲ組肉雞的平均日增重分別比對(duì)照組提高1.85%(P0.05)、6.84%(P0.05)和4.74%(P0.05)。在試驗(yàn)的1-14日齡階段、14-28日齡階段和28-42日齡階段,試驗(yàn)Ⅱ組肉雞平均日增重分別比對(duì)照組提高2.86%(P0.05)、3.74%(P0.05)和10.58%(P0.05),隨著日齡的增加,肉雞平均日增重提高的幅度越大,且均高于試驗(yàn)Ⅰ、Ⅲ組,。在試驗(yàn)的各個(gè)階段,不同水平短鏈酮酸對(duì)肉雞料重比無(wú)顯著影響(P0.05)。2.不同水平短鏈酮酸對(duì)AA肉雞血清生化指標(biāo)的影響:各試驗(yàn)組肉雞血清中總蛋白和球蛋白含量均高于對(duì)照組(P0.05)。試驗(yàn)Ⅲ組肉雞血清中白球比顯著低于對(duì)照組(P0.05),其他各組間差異不顯著(P0.05)。與對(duì)照組相比,各試驗(yàn)組肉雞血清中高密度脂蛋白含量升高,甘油三酯含量降低,并且均以試驗(yàn)Ⅱ組的變化幅度最大(P0.05)。各組肉雞血清白蛋白含量、總膽固醇含量和低密度脂蛋白含量差異不顯著(P0.05)。3.不同水平短鏈酮酸對(duì)AA肉雞免疫指標(biāo)的影響:短鏈酮酸對(duì)肉雞免疫器官指數(shù)無(wú)顯著影響(P0.05)。與對(duì)照組相比,各試驗(yàn)組肉雞血清中免疫球蛋白、補(bǔ)體含量升高(P0.05),其中試驗(yàn)Ⅲ組肉雞血清中補(bǔ)體C3含量顯著高于對(duì)照組(P0.05),試驗(yàn)Ⅱ組肉雞血清中免疫球蛋白Ig A、Ig M和補(bǔ)體C4含量高于試驗(yàn)Ⅰ和Ⅲ組(P0.05)。4.不同水平短鏈酮酸對(duì)AA肉雞抗氧化指標(biāo)的影響:短鏈酮酸均可提高肉雞血清和肝臟中總抗氧化能力,降低丙二醛含量,其中試驗(yàn)Ⅲ組肉雞肝臟總抗氧化能力顯著高于對(duì)照組(P0.05)。試驗(yàn)Ⅰ、Ⅱ、Ⅲ組肉雞血清谷胱甘肽過(guò)氧化物酶活性分別比對(duì)照組高13.95%(P0.05)、30.04%(P0.05)和31.56%(P0.05)。隨著短鏈酮酸添加水平的增加,各試驗(yàn)組肉雞肝臟超氧化物歧化酶活性增強(qiáng)(P0.05)。綜合以上各項(xiàng)指標(biāo)得出:日糧中添加短鏈酮酸可促進(jìn)肉雞采食,改善免疫功能,增強(qiáng)機(jī)體抗氧化性能,提高肉雞增重和飼料轉(zhuǎn)化率。本試驗(yàn)條件下肉雞日糧中短鏈酮酸產(chǎn)品的適宜添加量為0.500%。
[Abstract]:Short chain keto acid is an intermediate product of the regulation of metabolism, as a new type of feed additives, has the advantage of no residue, which caused widespread interest in the domestic and foreign animal husbandry workers. Short chain acid has certain growth promoting action, but because the animal species and the growth stage is different, its level is not added the same. Based on the previous study of our laboratory, the experiment selects the AA broilers as experimental animal, to observe the effects of different levels of short chain acid on growth performance and blood index of AA broiler, determine the AA in broiler diets and the optimum level, and provide a theoretical basis and application parameters for the application in the actual production of short chain acid the test. Selected health, there was no significant difference between the initial weight of 1 day old AA meat 192 cocks were randomly divided into 4 treatments, which were control group, group I, group II test, test group, each with 4 Repeat, 12 hens in each replicate. The control group was fed basal diet, test group I, II, III added 0.375% respectively in the basic diet, the 0.500% and 0.625% short chain acid products. The experiment lasted for 42 days. During the test every 2 weeks to test a chicken were weighed and to repeat units the remainder were called back, to calculate average daily gain, average daily feed intake and feed conversion ratio. In twenty-ninth days, a total of 1 close to the average weight of chickens from each replicate, after weighing fasting blood was collected via jugular vein, isolated serum were measured blood index, slaughtered after spleen, thymus and bursa of Fabricius were weighed. The results show that the effect of 1. different levels of short chain acid on growth performance of broilers in each experimental group: AA broilers at the age of 14 days, 28 days and 42 days when the weight is higher than the control group, the experimental group I, II broiler body weight at the age of 28 days to reach show 钁楁按騫,
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