發(fā)布時(shí)間：2018-04-14 06:28

  本文選題：BPTF + BRG��； 參考：《畜牧獸醫(yī)學(xué)報(bào)》2017年03期

【摘要】：旨在研究染色質(zhì)重塑因子BPTF、BRG1在不同毛色綿羊皮膚的表達(dá)與定位。采集黑色和白色各3只綿羊的背部皮膚組織,用qRT-PCR檢測不同毛色皮膚中BPTF和BRG1mRNA相對表達(dá)量,免疫組化技術(shù)定位分析,Western blotting半定量研究BPTF和BRG1蛋白相對表達(dá)量。qRT-PCR結(jié)果表明,BPTF基因在黑色綿羊皮膚中的mRNA相對表達(dá)量顯著高于白色綿羊(P0.05),BRG1基因在黑色綿羊皮膚中的mRNA相對表達(dá)量極顯著高于白色綿羊(P0.01);Western blotting結(jié)果表明,黑色綿羊的皮膚中BPTF蛋白表達(dá)量顯著高于白色綿羊(P0.05),BRG1蛋白表達(dá)量極顯著高于白色綿羊(P0.01);免疫組化結(jié)果表明,BPTF蛋白和BRG1蛋白在綿羊皮膚中毛囊的毛根鞘及毛球部均有表達(dá)。綜上表明,BPTF和BRG1基因可能參與綿羊毛色形成過程。
[Abstract]:The aim of this study was to investigate the expression and localization of chromatin remodeling factor BPTF- BRG1 in sheep skin.The back skin tissues of 3 black sheep and 3 white sheep were collected, and the relative expression of BPTF and BRG1mRNA were detected by qRT-PCR.Expression of BPTF and BRG1 protein in Black Sheep skin by Immunohistochemistry and Semi-quantitative Analysis of Western blotting. QRT-PCR results showed that the relative expression of mRNA gene in black sheep skin was significantly higher than that in black sheep skin.The relative expression of mRNA was significantly higher than that of P0.01 in white sheep.The expression of BPTF protein in the skin of black sheep was significantly higher than that in the white sheep, and the expression of BPTF protein and BRG1 protein in the hair follicle of sheep was significantly higher than that in the white sheep P0.01.The immunohistochemical results showed that the expression of BPTF protein and BRG1 protein in the hair follicle and hair follicle of the sheep were all positive.It is concluded that BPTF and BRG1 genes may be involved in the process of wool color formation.
【作者單位】： 山西農(nóng)業(yè)大學(xué)動物科技學(xué)院;蚌埠市和平乳業(yè)有限公司;
【基金】：國家自然科學(xué)基金(30972223;31272628)
【分類號】：S826.2

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本文編號：1748129