本文選題：乳酸菌培養(yǎng)物　切入點(diǎn)：肉雞　出處：《吉林農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：本試驗(yàn)采用正交試驗(yàn)方法,通過測(cè)定三種乳酸菌發(fā)酵液的抑菌活性,篩選出三種對(duì)致病菌有抑制作用的乳酸菌培養(yǎng)物,研究其對(duì)肉雞生產(chǎn)性能、消化機(jī)能、盲腸微生物、免疫功能、抗氧化性能及血清生化指標(biāo)的影響。采用單因子完全隨機(jī)化試驗(yàn)設(shè)計(jì),將體重相近的健康1日齡AA肉仔雞300只隨機(jī)分配到5個(gè)處理組中。其中對(duì)照組(C)飼喂基礎(chǔ)日糧,抗生素組(A)在基礎(chǔ)日糧中添加0.01%金霉素,試驗(yàn)組L1、L2、L3分別在基礎(chǔ)日糧中添加0.16%乳酸菌培養(yǎng)物L(fēng)1、L2、L3。每個(gè)處理6個(gè)重復(fù),每個(gè)重復(fù)10只雞。試驗(yàn)期為42天,分別于21日齡和42日齡屠宰取樣。結(jié)果表明乳酸菌培養(yǎng)物L(fēng)1顯著提高肉雞平均日采食量(P0.01)和料重比(P0.05),L3顯著提高試驗(yàn)前期平均日增重(P0.01),L1和L2提高試驗(yàn)后期平均日增重(P0.01)。日糧中添加乳酸菌培養(yǎng)物L(fēng)1能顯著提高試驗(yàn)前期肉雞免疫器官指數(shù)、盲腸乙酸濃度和血清中SOD活性,顯著提高試驗(yàn)后期肝臟、胰腺指數(shù)、磷消化率(P0.05)和血清總蛋白、白蛋白數(shù)量及ALP活性(P0.01)。日糧中添加乳酸菌培養(yǎng)物L(fēng)2能顯著提高試驗(yàn)前期肉雞血清總蛋白、白蛋白數(shù)量(P0.01),顯著提高整個(gè)試驗(yàn)期肝臟指數(shù)及試驗(yàn)后期肉雞免疫器官指數(shù)、盲腸丙酸和總VFA濃度、血清新城疫抗體效價(jià)、T-AOC和ALP活性(P0.05)。日糧中添加乳酸菌培養(yǎng)物L(fēng)3能顯著提高試驗(yàn)前期肉雞粗脂肪消化率、脾臟指數(shù)和血清中SOD活性,顯著提高試驗(yàn)后期胸腺指數(shù)、血清中IgA含量(P0.05)和整個(gè)試驗(yàn)期血清中ALP活性(P0.01)。日糧中添加乳酸菌培養(yǎng)物能顯著降低盲腸乳酸桿菌數(shù)量(P0.05)和大腸桿菌數(shù)量(P0.01),顯著提高盲腸雙歧桿菌數(shù)量和試驗(yàn)后期氮的表觀沉積率(P0.05)。綜上所述,在日糧中添加滅活的乳酸菌培養(yǎng)物,L3的抑菌效果最好,試驗(yàn)前期添加量適宜,試驗(yàn)后期添加量應(yīng)適當(dāng)降低;L1和L2的抑菌效果較差,試驗(yàn)前期添加量應(yīng)適當(dāng)增加,試驗(yàn)后期添加量適宜。在肉雞生產(chǎn)中,可用0.16%滅活乳酸菌培養(yǎng)物替代抗生素,從而達(dá)到更好的經(jīng)濟(jì)效果。
[Abstract]:By means of orthogonal test, three kinds of lactic acid bacteria culture were screened out by determining the bacteriostatic activity of three kinds of lactic acid bacteria fermentation broth, and the performance, digestion function and cecum microorganism of three kinds of lactic acid bacteria culture were studied. Effects of immune function, Antioxidant activity and Serum biochemical Indexes. Three hundred healthy 1-day-old AA broilers of similar body weight were randomly assigned to five treatment groups. The control group (C) was fed with basal diet, the antibiotic group (A) added 0.01% chlortetracycline into the basal diet. In the trial group, L1 + L2N L3 was added to the basal diet with 0.16% lactic acid bacteria culture L1 / L2 + L3. Each treatment included 6 replicates and 10 chickens per replica. The trial period was 42 days. The results showed that Lactic acid bacteria culture L1 significantly increased the average daily feed intake (P 0.01) and feed weight ratio (P 0.05) L 3 significantly increased the average daily gain (P 0.01 L 1 and L 2) in the early stage of the experiment. Lactic acid bacteria culture L1 could significantly increase the immune organ index of broilers in the early stage of the experiment. The concentration of acetic acid in cecum and the activity of SOD in serum significantly increased liver, pancreas index, phosphorus digestibility (P 0.05) and serum total protein. The amount of albumin and the activity of ALP were significantly increased by adding lactic acid bacteria culture L2 to the diet. The serum total protein and albumin content of broilers were significantly increased, and the liver index and immune organ index of broilers were significantly increased during the whole trial period. The concentration of propionic acid and total VFA in the caecum, the titer of serum Newcastle disease antibody, the activity of T-AOC and the activity of ALP were P0.050.The dietary supplementation of Lactic acid bacteria culture L3 could significantly increase the digestibility of crude fat, spleen index and the activity of SOD in serum of broilers in the early stage of the experiment. The thymus index was significantly increased in the later stage of the trial. The content of IgA in serum and the activity of ALP in serum during the whole trial period were P0.010.The addition of lactic acid bacteria to the diet significantly decreased the number of Lactobacillus caecum and Escherichia coli, and significantly increased the number of bifidobacterium cecum and the later stage of the experiment. The apparent deposition rate of nitrogen is P0.050.In summary, The bacteriostatic effect of inactivated lactic acid bacteria culture (L3) was the best, the amount of L3 was suitable in the early stage of the experiment, the addition amount of L2 and L2 should be reduced in the later stage of the experiment, and the amount added in the early stage of the experiment should be increased appropriately. In the later stage of the experiment, the antibiotics could be replaced by 0.16% inactivated lactic acid bacteria culture in the broiler production, thus achieving a better economic effect.
【學(xué)位授予單位】：吉林農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S831.5

【參考文獻(xiàn)】

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1 王全溪;林n，

本文編號(hào)：1677612