本文選題：禽網(wǎng)狀內(nèi)皮組織增殖病病毒　切入點(diǎn)：雞傳染性法氏囊病活疫苗　出處：《中國農(nóng)業(yè)科學(xué)院》2015年碩士論文

【摘要】：H5亞型禽流感病毒(AIV)引起的高致病性禽流感(HPAI)給養(yǎng)禽業(yè)帶來重大經(jīng)濟(jì)損失,也威脅著人類的公共衛(wèi)生安全。中國采用免疫加撲殺相結(jié)合的綜合措施防控禽流感(AI),疫苗免疫在防控AI方面取得了顯著成效。然而,當(dāng)前HPAI還時(shí)有發(fā)生,在疫苗免疫方面主要存在著個(gè)別雞群疫苗免疫保護(hù)效果與實(shí)驗(yàn)室結(jié)果相差較大的問題。影響免疫效果的因素很多,本研究主要探討免疫抑制性病毒中禽網(wǎng)狀內(nèi)皮組織增殖病病毒(REV)和雞傳染性法氏囊病(IBD)活疫苗株對機(jī)體的免疫抑制作用,為分析AI疫苗免疫效果不理想的原因及IBD疫苗的選用提供參考依據(jù)。為研究REV對AI疫苗免疫效果的影響,本研究在免疫重組禽流感病毒滅活疫苗(簡稱AI滅活苗)或禽流感-新城疫重組二聯(lián)活疫苗(簡稱新禽活苗)之前以REV經(jīng)典分離株感染1日齡SPF雞,與未感染REV的免疫對照雞一起,分別進(jìn)行血清抗體監(jiān)測、外周血及脾臟淋巴細(xì)胞增殖指數(shù)(SI)測定、外周血及脾臟淋巴細(xì)胞CD4+/CD8+值測定、細(xì)胞因子水平檢測、攻毒后發(fā)病和死亡情況及排毒測定。結(jié)果顯示,REV顯著抑制免疫初期HI抗體、T淋巴細(xì)胞增殖能力和CD4+/CD8+值;平均HI抗體峰值相差4倍以上,同一免疫時(shí)間HI抗體最高相差達(dá)64倍;雞只感染REV后,AI滅活苗僅提供70%的保護(hù)率,一次免疫和兩次免疫新禽活苗提供的保護(hù)率分別為60%和80%,而未感染REV的相應(yīng)免疫雞獲得100%保護(hù)。為評價(jià)IBD活疫苗對AI疫苗免疫效果的影響,本研究選取弱毒力(Gt株)和中等毒力(MB株)IBD活疫苗,分別與AI滅活苗或新禽活苗一起免疫SPF雞,與僅免疫相應(yīng)AI疫苗的雞一起,進(jìn)行與REV對AI疫苗免疫效果研究同樣指標(biāo)的檢測。結(jié)果顯示,IBD活疫苗(Gt株)加AI滅活苗、IBD活疫苗(MB株)加AI滅活苗與AI滅活苗免疫3組免疫雞免疫21天時(shí)平均HI抗體分別為5.9 log2、2.1 log2和7.7 log2,攻強(qiáng)毒后存活數(shù)分別為10/10、8/10和10/10,排毒數(shù)別為1/10、7/10和0/10。接種IBD活疫苗(Gt株)加新禽活苗、IBD活疫苗(MB株)加新禽活苗與新禽活苗免疫21天HI抗體分別為2.2 log2、1.6 log2和2.5 log2,攻強(qiáng)毒后存活數(shù)分別為10/10、7/10和10/10,排毒數(shù)分別為1/10、3/10和0/10。這兩種IBD活疫苗對細(xì)胞免疫反應(yīng)均無顯著影響。本研究表明,REV、IBD活疫苗(MB株)均對AI疫苗免疫效果產(chǎn)生顯著影響,而IBD活疫苗(Gt株)對AI疫苗攻毒保護(hù)免疫效果無明顯影響。
[Abstract]:H5 subtype avian influenza virus (AIV), a highly pathogenic avian influenza virus (HPAI), caused significant economic losses to the poultry industry. It is also a threat to human public health and safety. China uses a comprehensive measure of immunization plus culling to prevent and control bird flu. Vaccine immunization has achieved remarkable results in the prevention and control of AI. However, at present, HPAI still occurs from time to time. In the aspect of vaccine immunization, there is a big difference between the immune protection effect of individual chicken group vaccine and the result of laboratory. There are many factors that affect the immune effect. The aim of this study was to investigate the immunosuppressive effects of live vaccines against avian reticuloendotheliosis virus (Rev) and chicken infectious bursal disease (IBD) vaccine. In order to analyze the reason why the immune effect of AI vaccine is not ideal and the selection of IBD vaccine, and to study the effect of REV on the immune effect of AI vaccine, In this study, 1 day old SPF chickens were infected with REV classical isolates before the recombinant inactivated avian influenza virus vaccine (AI inactivated vaccine for short) or the recombinant live vaccine of avian influenza and Newcastle disease (new live vaccine). Serum antibodies, proliferation index (SI) of peripheral blood and spleen lymphocytes, CD4 / CD8 value of peripheral blood and spleen lymphocytes, and cytokine levels were measured in both peripheral blood and spleen lymphocytes. The results showed that Rev significantly inhibited the proliferation of T lymphocytes and the CD4 / CD8 value of HI antibody in the early stage of immunization, the difference of the peak value of HI antibody was more than 4 times, and the highest difference of HI antibody in the same immune time was 64 times. The inactivated AI vaccine of chickens infected with REV only provided 70% protection rate. In order to evaluate the effect of live IBD vaccine on AI vaccine, 60% and 80% protection rates were provided by single immunization and twice immunization respectively, while the corresponding chickens without REV were protected by 100%. In this study, SPF chickens were immunized with inactivated AI vaccine or new live bird vaccine, respectively, and chickens immunized with AI vaccine only. The results showed that inactivated AI vaccine plus live AI vaccine (MB strain) plus AI inactivated vaccine plus AI inactivated vaccine and AI inactivated vaccine were immunized with AI inactivated vaccine and AI inactivated vaccine. The results showed that chickens were immunized with AI inactivated vaccine and AI inactivated vaccine on average HI at 21 days after immunization. The antibody numbers were 5.9log2an2.1 log2 and 7.7log2, respectively. The survival numbers after intensive attack were 10 / 10 / 8 / 10 and 10 / 10, respectively, and the number of detoxification were 1 / 10 / 7 / 10 and 0 / 10 respectively. Inoculation of IBD live vaccine with IBD live vaccine (MB) plus new live bird vaccine and new live bird vaccine immunized with HI antibody for 21 days. The number of survival after intensive attack was 10 / 10 / 10 / 10 and 10 / 10 respectively, and the number of detoxification was 1 / 10 / 10 / 10 and 0 / 10 respectively. Neither of the two live IBD vaccines had significant effect on cellular immune response. The epidemic effect has a significant effect, The protective effect of AI vaccine against virus was not significantly affected by IBD live vaccine (Gt strain).
【學(xué)位授予單位】：中國農(nóng)業(yè)科學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S858.31

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