本文選題：兔精液　切入點：冷凍保存　出處：《西北農(nóng)林科技大學(xué)》2015年碩士論文

【摘要】：近年來,隨著經(jīng)濟的發(fā)展,人們對生活各方面的質(zhì)量和要求也越來愈高,首先就表現(xiàn)在飲食方面。我國是農(nóng)業(yè)大國,畜牧業(yè)是其中重要的組成部分,然而,傳統(tǒng)的高脂、高能的飲食習(xí)慣對我們的健康造成了很大的影響。而兔肉具有低脂肪、低膽固醇、低熱量的特點,能滿足人們的健康飲食。此外,我國人多地少,存在糧食供應(yīng)與需求的矛盾,豬、禽等都屬于耗糧型畜禽,與人爭糧的矛盾日益突出,而兔屬于節(jié)糧型家畜,可以有效的利用飼草資源,以節(jié)省糧食。因此,大力發(fā)展養(yǎng)兔業(yè)對促進(jìn)我國經(jīng)濟發(fā)展有重要作用。但是,公兔具有夏季不育的現(xiàn)象,在高溫?zé)釕?yīng)激下,會導(dǎo)致精液量下降,精子密度減少,品質(zhì)下降等。因此,需要將兔精液冷凍保存,以解決夏季無法配種的問題。然而,目前兔精液冷凍保存的效果并不理想,因此,尚未被廣泛的應(yīng)用于生產(chǎn)實踐中。為了進(jìn)一步改善冷凍保存后兔精液品質(zhì),本試驗從抗氧化劑方面對稀釋液進(jìn)行改進(jìn),通過向冷凍保存液中添加水溶性維生素E類似物(Trolox),來研究其對兔精液冷凍保存后精子品質(zhì)的影響。試驗以冷凍-解凍后精子活力、質(zhì)膜完整性、頂體完整性、DNA完整性、脂質(zhì)過氧化水平以及線粒體活性等作為評價指標(biāo),利用碘化丙錠(PI)、SYBR-14、異硫氰酸熒光素標(biāo)記的花生凝集素(FITC-PNA)、吖啶橙(AO)、BODIPY-C11熒光探針、5,5',6,6'-四氯-1,1',3,3'-四乙基苯丙咪唑碘化羧花青(JC-l)等對精子的質(zhì)量進(jìn)行評估。本文所得結(jié)果如下:1.兔精液冷凍保存液中添加Trolox,解凍后各試驗組的精子活力、頂體完整性、線粒體活性均顯著高于對照組(P㩳0.05),脂質(zhì)過氧化水平均顯著低于對照組(P㩳0.05),添加濃度為200μM時,質(zhì)膜完整性顯著高于對照組(P㩳0.05)。2.比較不同濃度的Trolox對精液品質(zhì)的影響,解凍后添加濃度為200μM的試驗組精子活力、頂體完整性、線粒體活性均顯著高于濃度為100μM和250μM的試驗組(P㩳0.05),濃度為100μM和200μM的試驗組其脂質(zhì)過氧化水平顯著低于250μM的試驗組(P㩳0.05)。本試驗結(jié)果表明維生素E類似物能增加兔精子的耐凍性,并且維生素E類似物最適添加濃度為200μM。
[Abstract]:In recent years, with the development of economy, people's quality and demand for all aspects of life have become higher and higher, first of all, in the aspect of diet. China is a big agricultural country, animal husbandry is an important part of it, however, the traditional high fat, High energy eating habits have a great impact on our health. Rabbit meat has the characteristics of low fat, low cholesterol and low calorie, which can satisfy people's healthy diet. In addition, there is a contradiction between food supply and demand. Pigs and poultry are all food consuming animals and poultry, and the contradiction of competing with people for grain is increasingly prominent, while rabbits are grain saving livestock, which can effectively use forage resources in order to save grain. The development of rabbit breeding industry plays an important role in promoting the economic development of our country. However, the male rabbit is sterile in summer. Under the high temperature heat stress, it will lead to the drop of spermatozoa, the decrease of sperm density, the decline of quality, etc. It is necessary to cryopreservation rabbit semen in order to solve the problem of inbreeding in summer. However, at present, the effect of cryopreservation of rabbit semen is not satisfactory, so, In order to further improve the quality of rabbit semen after cryopreservation, the diluent was improved in terms of antioxidants. To study the effect of water soluble vitamin E analogue troloxane on the sperm quality after cryopreservation of rabbit semen, the sperm motility, plasma membrane integrity, acrosomal integrity and DNA integrity were studied after freezing and thawing. Lipid peroxidation level and mitochondrial activity were used as evaluation indexes. The quality of spermatozoa was evaluated by using the fluorescent probe PIH4 SYBR-14, fluorescein isothiocyanate labeled peanut agglutinin (FITC-PNAA), acridine orange (Acridine Orange AOA) BODIPY-C11 as a fluorescence probe of 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 4, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 4, 5, 5, 5, 4, 5, 4, 4, 4, 4, Troloxx was added to the semen cryopreservation solution, and the sperm motility of the thawed groups was determined. Acrosomal integrity and mitochondrial activity were significantly higher than those of the control group. The level of lipid peroxidation was significantly lower than that of control group. When the concentration was 200 渭 M, the integrity of plasma membrane was significantly higher than that of the control group. After thawing, the sperm motility, acrosome integrity and mitochondrial activity were significantly higher in the thawed group than those in the 100渭 M and 250渭 M groups, and the sperm motility, acrosome integrity and mitochondrial activity in the thawed group were significantly higher than those in the control group (100 渭 M and 250 渭 M, respectively). The levels of lipid peroxidation in 100 渭 M and 200 渭 M groups were significantly lower than those in 250 渭 M group. The results showed that vitamin E analogue could increase the freezing resistance of rabbit spermatozoa, and the optimum concentration of vitamin E analogue was 200 渭 M.
【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S829.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 趙曉娥,王光亞,王希朝;布爾山羊精液保存條件的試驗研究[J];動物醫(yī)學(xué)進(jìn)展;1999年02期

，

本文編號：1677247