本文選題：肉仔雞　切入點：甘氨酸亞鐵　出處：《東北農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：鐵是動物生長發(fā)育所需的微量元素,甘氨酸螯合鐵的吸收和作用效果是動物營養(yǎng)研究的熱點。本試驗主要用同等鐵水平的甘氨酸亞鐵替代日糧中的硫酸亞鐵,測定0-21日齡肉仔雞的生長性能、生理生化指標和與鐵吸收利用相關(guān)的基因表達量。旨在研究混合鐵源對肉仔雞飼喂效果的影響,確定一個合適的甘氨酸亞鐵替代硫酸亞鐵比例,為實際生產(chǎn)中甘氨酸亞鐵在肉仔雞日糧中的使用提供依據(jù)。本試驗選用初始體重經(jīng)方差分析差異不顯著的1日齡AA肉公雞600只,按單因子完全隨機分組設(shè)計將600只肉仔雞隨機分成6個處理組(每個處理組分5次重復(fù),每個重復(fù)20只雞),1個組為對照組,5個組為試驗組。對照組日糧:基礎(chǔ)日糧添加硫酸亞鐵組成,添加水平為100mg/kg(以鐵計)。試驗組日糧:用甘氨酸亞鐵替代對照組日糧中的硫酸亞鐵組成,以同等鐵水平的甘氨酸亞鐵替代對照組日糧中硫酸亞鐵的20%、40%、60%、80%和100%,組成20%、40%、60%、80%和100%替代組。試驗周期為21天,待試驗結(jié)束后,對試驗肉仔雞進行稱重,采集血樣,屠宰試驗肉仔雞,采集肉仔雞的心臟、肝臟、脾臟、胸腺、法氏囊和十二指腸等組織,用以測定肉仔雞的生長性能、免疫器官指數(shù)、組織鐵含量、血液生理生化指標、抗氧化酶活性和與鐵吸收利用相關(guān)的基因表達量等指標。試驗結(jié)果表明:用等鐵水平的甘氨酸亞鐵替代日糧中的硫酸亞鐵對0-21日齡肉仔雞的平均日增重、平均日采食量及料重比等生產(chǎn)指標和胸腺、脾臟及法氏囊等免疫器官指數(shù)影響不顯著(P0.05),但隨著甘氨酸亞鐵替代比例的升高,0-21日齡肉仔雞的胸腺指數(shù)有逐漸增加的趨勢;用等鐵水平的甘氨酸亞鐵替代日糧中的硫酸亞鐵對0-21日齡肉仔雞的心臟鐵含量、肝臟鐵含量和脾臟鐵含量均有顯著的影響(P0.05),隨著甘氨酸亞鐵替代比例的升高,0-21日齡肉仔雞的各組織鐵含量明顯升高,但60%替代組、80%替代組和100%替代組三組之間0-21日齡肉仔雞的各組織鐵含量差異均不顯著(P0.05);用等鐵水平的甘氨酸亞鐵替代日糧中的硫酸亞鐵對0-21日齡肉仔雞的血液生理生化指標有顯著影響(P0.05),隨著甘氨酸亞鐵替代比例的升高,0-21日齡肉仔雞的血紅蛋白含量、紅細胞計數(shù)、紅細胞壓積、血清鐵蛋白含量都顯著提高,且血清總鐵結(jié)合力顯著降低,但60%替代組、80%替代組和100%替代組三組之間0-21日齡肉仔雞的各個血液生理生化指標差異均不顯著(P0.05);0-21日齡肉仔雞血清過氧化氫酶的活性隨甘氨酸亞鐵替代比例的增加而升高,且差異顯著(P0.05),但用等鐵水平的甘氨酸亞鐵替代日糧中的硫酸亞鐵對0-21日齡肉仔雞血清黃嘌呤氧化酶和肝臟超氧化物歧化酶的活性均無顯著差異(P0.05);用等鐵水平的甘氨酸亞鐵替代日糧中的硫酸亞鐵顯著提高了0-21日齡肉仔雞肝臟Hepcidin mRNA相對表達量(P0.05),并且顯著降低了對0-21日齡肉仔雞十二指腸DMT1 mRNA相對表達量(P0.05),但60%替代組、80%替代組和100%替代組三組之間對0-21日齡肉仔雞的肝臟Hepcidin mRNA和十二指腸DMT1 mRNA相對表達量差異均不顯著(P0.05)。綜合本試驗結(jié)果認為:在肉仔雞生長前期,在日糧中用甘氨酸亞鐵以1:1比例替代硫酸亞鐵(以鐵計)是可行的;從降低成本和提高鐵的吸收利用等方面考慮,最佳替代比例為60%。
[Abstract]:Iron is a trace element in animal growth required for the absorption and the effect of ferrous glycinate is a hot research on animal nutrition. The main test instead of diets with the same level of iron in the ferrous sulfate ferrous glycinate, determination of the growth performance of 0-21 day old broilers, physiological and biochemical indexes and using the related gene expression with iron absorption. To study the mixed iron source feeding effect on broilers, determine an appropriate proportion of ferrous sulfate and ferrous glycine substitution, for use in actual production of ferrous glycine in broiler diets provide the basis. The initial weight variance analysis showed no significant difference of the 1 day old AA broilers cock 600, according to the single factor completely randomized design 600 broilers were randomly divided into 6 groups (each group divided into 5 replicates of 20 chicks per replicate), 1 group, 5 group The test group. The control group diets: basal diet supplemented with ferrous sulfate. The adding level was 100mg/kg (with Fe). Diets with ferrous glycine substitution control diet of ferrous sulfate, with the same level of iron sulfur acid ferrous ferrous glycine substitution control diet in 20%. 40%, 60%, 80% and 100%, 20%, 40%, 60%, 80% and 100% replacement group. The test period is 21 days, until after the end of the trial, the test of broilers were weighed and collected blood samples, broiler slaughter test, collecting broiler heart, liver, spleen, thymus, bursa and duodenum the tissues used to determine the growth performance of broilers, index of immune organs, tissues, blood physiological and biochemical index, antioxidant enzyme activity and iron absorption and utilization index and related gene expression. Experimental results show that the level of iron in the diet instead of ferrous glycine sulfate etc. Iron of 0-21 day old broilers average daily gain, average daily feed intake and ratio of feed production index and thymus index of immune organs, spleen and bursa is not significantly affected (P0.05), but with the increase of ferrous glycine substitution ratio of 0-21 day old broiler thymus index increased gradually heart; iron levels of ferrous glycine replacement of dietary ferrous sulfate of 0-21 day old broilers were used, significantly affected the contents of iron in the liver and spleen iron (P0.05), with the increase of ferrous glycine substitution ratio, iron content in different tissues of 0-21 day old broiler was significantly increased, but 60% alternative group, the difference of iron content in different tissues of 80% replacement group and 100% replacement group between the three groups of 0-21 day old broilers were not significant (P0.05); the level of iron in the diet of ferrous glycine substitution of ferrous sulfate on the 0-21 day old broiler blood etc. The physiological and biochemical indicators have significant effects (P0.05), with the increase of ferrous glycine substitution ratio, hemoglobin content of 0-21 day old broiler, red blood cell count, hematocrit, serum ferritin levels were significantly increased, and serum total iron binding capacity decreased significantly, but for the 60% generation group, 80% replacement group and 100% instead of the 0-21 day old broiler blood physiological biochemical indexes of various differences between the three groups was not significant (P0.05); 0-21 increase in broilers serum catalase activity with ferrous glycine substitution ratio increased, and the difference was significant (P0.05), but the level of iron in the diet of ferrous glycine substitution ferrous sulfate for 0-21 day chicken serum and liver xanthine oxidase at meat superoxide dismutase activity had no significant difference (P0.05); with the iron level in the diet instead of ferrous glycine sulfate iron significantly increased at 0-21 days of age The relative expression of broiler liver Hepcidin mRNA (P0.05), and significantly reduced to 0-21 day old broiler duodenum DMT1 relative expression of mRNA (P0.05), but the 60% replacement group, 80% group and 100% replacement group between the three groups on the 0-21 day old broiler liver Hepcidin mRNA and DMT1 mRNA relative expression in duodenum there was no significant difference (P0.05). The results showed that at the early growth stage of broilers, with ferrous glycine in the diets with a ratio of 1:1 to replace ferrous sulfate (with Fe) is feasible; the lower the cost and improve the absorption and utilization of iron and other aspects of consideration, best replacement ratio of 60%.

【學(xué)位授予單位】：東北農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S831.5

【參考文獻】

相關(guān)期刊論文 前3條

1 周桂蓮;氨基酸螯合鐵的研究與應(yīng)用進展[J];飼料博覽;1998年06期

2 鞠繼光;微量元素有機螯合物對生長肥育豬生長性能的影響[J];飼料博覽;2001年01期

3 蔡輝益 ,B·Vandergrift;飼料工業(yè)中微量元素—氨基酸螯合鹽的理論與實踐[J];國外畜牧學(xué)(飼料);1994年06期

，

本文編號：1677014