本文選題：泰勒蟲　切入點(diǎn)：流行情況　出處：《塔里木大學(xué)》2017年碩士論文

【摘要】：羊泰勒蟲病是經(jīng)媒介蜱傳播的一種血液原蟲病,該病主要可引起感染動(dòng)物高熱、體表淋巴結(jié)腫大、貧血和消瘦等癥狀,嚴(yán)重者可致死亡。該病呈地方流行性,尤其對(duì)我國西北地區(qū)養(yǎng)羊業(yè)危害較大。國內(nèi)外有關(guān)羊血液內(nèi)泰勒蟲病的流行情況和檢測(cè)方法研究較多,但對(duì)于新疆南疆地區(qū)綿羊的泰勒蟲病感染情況和病原種類的報(bào)道尚較少。本研究旨在應(yīng)用顯微鏡檢查和PCR技術(shù)查明該區(qū)域綿羊血液中泰勒蟲的感染情況,并鑒定其病原種類,以期為養(yǎng)羊業(yè)中羊泰勒蟲病的診斷和防治提供科學(xué)依據(jù)。1.為了解喀什地區(qū)綿羊泰勒蟲病的流行情況,于2015年11月至2016年1月,采用血涂片鏡檢法對(duì)喀什11個(gè)(區(qū)、縣)258只當(dāng)?shù)爻赡昃d羊的血液樣品進(jìn)行檢查。結(jié)果發(fā)現(xiàn)泰勒蟲陽性樣品151份,泰勒蟲總感染率為59.0%。不同采樣點(diǎn)綿羊的泰勒蟲感染率存在差異,其中莎車綿羊的泰勒蟲感染率最高達(dá)75%,而喀什市綿羊的泰勒蟲感染率較低,為8.3%。調(diào)查結(jié)果表明,喀什地區(qū)綿羊的泰勒蟲病普遍流行,應(yīng)加強(qiáng)對(duì)該病的綜合防治。2.為進(jìn)一步掌握新疆南疆綿羊的泰勒蟲感染情況及其種類�；谔├障x通用引物,采用PCR法對(duì)該區(qū)域637份綿羊血液DNA樣品進(jìn)行檢測(cè),發(fā)現(xiàn)泰勒蟲陽性樣品400份,總感染率為62.8%。不同采樣點(diǎn)綿羊的泰勒蟲感染率存在差異,其中,莎車、英吉沙和澤普綿羊的泰勒蟲感染率均高達(dá)100%,沙雅綿羊的泰勒蟲感染率較低,為4.0%。分別用綿羊泰勒蟲、尤氏泰勒蟲和呂氏泰勒蟲特異性引物對(duì)以上400份泰勒蟲陽性樣品進(jìn)行擴(kuò)增,結(jié)果顯示,綿羊泰勒蟲特異性引物擴(kuò)增出399個(gè)陽性,而尤氏泰勒蟲和呂氏泰勒蟲均未擴(kuò)增出。1份樣品(樣品編號(hào)為1443)在通用引物位點(diǎn)鑒定為陽性,但在3種特異性引物均未擴(kuò)增出條帶。3.隨機(jī)選取PCR檢測(cè)為綿羊泰勒蟲的陽性樣品中的56個(gè)樣本和1443號(hào)樣本進(jìn)行測(cè)序,成功獲得55個(gè)綿羊泰勒蟲序列和1443號(hào)樣本序列。在NCBI中進(jìn)行Blast比對(duì),應(yīng)用Clustal X 2.11軟件分別進(jìn)行比對(duì)及序列拼接。發(fā)現(xiàn)所有綿羊泰勒蟲序列均一致,與來自的伊朗的綿羊源綿羊泰勒蟲(GenBank序列登錄號(hào)為KX273858)、土耳其的綿羊源綿羊泰勒蟲(KU342701)、中國的綿羊源綿羊泰勒蟲(FJ603460)序列同源性為100%,鑒定為綿羊泰勒蟲。1443號(hào)樣本的序列與來自的伊朗的牛源環(huán)形泰勒蟲(KX273857)、印度的牛源環(huán)形泰勒蟲(KT736498)、中國的梅花鹿源環(huán)形泰勒蟲(KT959231)序列同源性為100%,鑒定為環(huán)形泰勒蟲。本研究應(yīng)用顯微鏡檢查和PCR技術(shù)對(duì)新疆南疆綿羊血液樣品進(jìn)行了泰勒蟲檢測(cè),發(fā)現(xiàn)該區(qū)域綿羊的泰勒蟲感染較為普遍,且發(fā)現(xiàn)了環(huán)形泰勒蟲。本研究為綿羊的泰勒蟲病的傳播、診斷和防治提供了參考依據(jù),并為綿羊泰勒蟲病的免疫學(xué)研究提供有益資料。
[Abstract]:Taylor's disease of sheep is a blood protozoa disease transmitted by vector ticks. It can cause high fever, enlarged lymph nodes, anemia and wasting in infected animals. In particular, it is harmful to sheep farming in northwest China. There are many studies on the prevalence and detection methods of Taylor's disease in sheep blood at home and abroad. However, there are few reports of Taylor's disease infection and pathogenic species in sheep in southern Xinjiang. The purpose of this study was to use microscope and PCR technique to find out the infection of Taylor's parasite in sheep blood and to identify its pathogenic species. In order to provide scientific basis for diagnosis and control of sheep Taylor's disease in sheep industry, in order to understand the epidemic situation of Taylor's disease in sheep in Kashi area, from November 2015 to January 2016, blood smear microscopy was used to detect 11 areas of Kashi. The blood samples of 258 local adult sheep were examined. The results showed that 151 positive samples of Taylor worm were positive and the total infection rate of Taylor worm was 59.0. There were differences in the infection rate of Taylor parasite in sheep from different sampling points. The highest rate of Taylor infection in sheep was 75 and the infection rate of Taylor worm in sheep in Kashi was 8.3.The results showed that Taylor's disease was prevalent in sheep in Kashi area. In order to further understand the situation and species of Taylor's infection in sheep in southern Xinjiang, 637 sheep blood DNA samples were detected by PCR method based on universal primer of Taylor worm. It was found that there were 400 positive samples of Taylor worm, and the total infection rate was 62.8. There were differences in the infection rate of Taylor parasite in sheep with different sampling points, among which, the infection rate of Taylor parasite was as high as 100% in Sharp, Ingisha and Zep sheep, and the infection rate of Taylor worm in Shaya sheep was lower than that in Shaya sheep. Four hundred positive samples of Taylor's worm were amplified by using specific primers of Taylor's worm, Taylor's worm of sheep and Taylor's worm of lui respectively. The results showed that 399 positive samples were amplified by specific primers of Taylor's worm of sheep. However, none of the 1. 1 samples (sample number 1443) was identified as positive by universal primer locus. However, no bands were amplified from the three specific primers. Random selection of 56 samples and 1443 samples from positive samples of Taylor's worm detected by PCR was carried out for sequencing. 55 sheep Taylor worm sequences and 1443 sample sequences were successfully obtained. Blast alignment was carried out in NCBI, and Clustal X 2.11 software was used to carry out alignment and sequence splicing respectively. It was found that all the sheep Taylor worm sequences were identical. The sequence homology with the GenBank sequence of the sheep from Iran was KX273858, that of Turkey from Turkey was KU342701, and that of China from China was 100%, and that it was identified as the sample of Taylor worm of sheep .1443. The homology of KX273857 with KX273857 from Iran, KT736498 from India and KT959231 from Chinese sika deer was 100. The blood samples of sheep in southern Xinjiang were tested for Taylor worm. It was found that the infection of Taylor's worm in sheep in this area was more common and the circular Taylor worm was found. This study provided a reference for the transmission diagnosis and control of Taylor's disease in sheep and provided useful information for the immunological study of Taylor's disease in sheep.
【學(xué)位授予單位】：塔里木大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S858.26

【共引文獻(xiàn)】

相關(guān)博士學(xué)位論文 前10條

1 戴冰;基于納米復(fù)合材料的生物傳感器的研制及性能分析[D];哈爾濱理工大學(xué);2016年

2 田慧卿;聚合物多腔微管擠出成型理論與模具設(shè)計(jì)方法[D];大連理工大學(xué);2016年

3 祁娜;聚合物微結(jié)構(gòu)超聲波壓印方法及機(jī)理研究[D];大連理工大學(xué);2016年

4 殷素峰;超薄注塑充模理論研究及其在導(dǎo)光板成型中的應(yīng)用[D];華南理工大學(xué);2014年

5 焦志偉;內(nèi)循環(huán)二板式精密注塑機(jī)關(guān)鍵技術(shù)的研究[D];北京化工大學(xué);2012年

6 劉瑩;基于微流控芯片的微結(jié)構(gòu)制品注塑成型工藝技術(shù)研究[D];大連理工大學(xué);2012年

7 楊鐸;聚合物熔體表面效應(yīng)與平板微器件的注塑成型研究[D];大連理工大學(xué);2011年

8 楊燦;注塑微結(jié)構(gòu)復(fù)制性能及形態(tài)研究[D];華南理工大學(xué);2011年

9 徐斌;微注塑充模流動(dòng)尺度效應(yīng)及其模具關(guān)鍵技術(shù)研究[D];大連理工大學(xué);2010年

10 盧振;微結(jié)構(gòu)件的注射成形及其組織與性能[D];哈爾濱工業(yè)大學(xué);2008年

相關(guān)碩士學(xué)位論文 前10條

1 黃永程;非球面透鏡注塑壓縮成型工藝參數(shù)優(yōu)化研究[D];昆明理工大學(xué);2016年

2 喻欣;A企業(yè)員工激勵(lì)機(jī)制研究[D];西南交通大學(xué);2015年

3 王國華;銀海燈具有限公司LED燈具營銷策略研究[D];山西大學(xué);2015年

4 張朋;塑料非球面透鏡注射壓縮模具設(shè)計(jì)及成型試驗(yàn)研究[D];大連理工大學(xué);2015年

5 倪文斌;大功率智能LED電源設(shè)計(jì)[D];華中科技大學(xué);2015年

6 劉倩倩;注射壓縮成型工藝過程的模擬仿真及模具設(shè)計(jì)[D];天津理工大學(xué);2015年

7 陰俊峰;LED光學(xué)模具超精密加工工藝研究[D];廣東工業(yè)大學(xué);2014年

8 孫慧媛;中國LED照明燈具的國際市場(chǎng)營銷策略研究[D];安徽大學(xué);2014年

9 譚國文;LED光學(xué)模具結(jié)構(gòu)設(shè)計(jì)與分析[D];廣東工業(yè)大學(xué);2014年

10 蔣豐澤;微透鏡陣列注射壓縮工藝與模具技術(shù)研究[D];中南大學(xué);2014年

，

本文編號(hào)：1661690