本文選題：牛病毒性腹瀉病毒　切入點：牛傳染性鼻氣管炎病毒　出處：《中國預防獸醫(yī)學報》2017年02期

【摘要】：為建立同時快速鑒別檢測牛病毒性腹瀉病毒(BVDV)、牛傳染性支氣管炎病毒(IBRV)和口蹄疫病毒(FMDV)的方法,本研究根據(jù)高度保守的BVDV的5'UTR基因、IBRV的g B基因和FMDV的3D基因,分別設計了3對對應的特異性引物和3種不同發(fā)光基團標記的Taq Man探針,建立了同時檢測這3種病毒的多重熒光定量PCR的方法。并對其反應條件進行優(yōu)化。結(jié)果表明,該多重熒光定量PCR方法能夠特異性檢測出BVDV、IBRV和FMDV,而對BTV等檢測結(jié)果均為陰性,對BVDV、IBRV、FMDV的最低檢測量分別為194拷貝/μL、208拷貝/μL和150拷貝/μL。而且組內(nèi)和組間變異系數(shù)均低于0.85%。本研究所建立的多重熒光定量PCR具有方便、快速、特異性好、靈敏度高、重復性好等優(yōu)點,能夠用于BVDV、IBRV和FMDV的同時檢測。
[Abstract]:In order to establish a method for rapid identification of bovine viral diarrhea virus (BV DVV), bovine infectious bronchitis virus (IBRV) and foot-and-mouth disease virus (FMDV) simultaneously, a highly conserved 5'UTR gene of BVDV, GB gene of IBRV, and 3D gene of FMDV, were used in this study. Three pairs of specific primers and three Taq Man probes labeled with different luminescence groups were designed, and a method for simultaneous detection of multiplex fluorescence quantitative PCR of these three viruses was established. The reaction conditions were optimized. The multiplex fluorescence quantitative PCR method can detect BV DVV IBRV and FMDV specifically, but the results of BTV and so on are negative. The lowest detection of IBRV FMDV was 194 copies / 渭 L, 208 copies / 渭 L and 150 copies / 渭 L. the coefficient of variation within and between groups was lower than 0.85. The multiplex fluorescence quantitative PCR established in this study was convenient, rapid, specific and sensitive. It can be used for simultaneous detection of BV DVV IBRV and FMDV.
【作者單位】： 沈陽農(nóng)業(yè)大學;中國動物疫病預防控制中心;
【分類號】：S852.65

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