本文選題：大蒜素　切入點：蛋雞　出處：《東北農(nóng)業(yè)大學》2015年碩士論文　論文類型：學位論文

【摘要】：本實驗以哈爾濱市某種雞場200只1日齡海蘭褐雌性蛋雛雞為研究對象,預飼一周后,隨機分為4組,每組50只蛋雛雞,分別飼喂含0(對照組)、50(低劑量組)、100(中劑量組)和150(高劑量組)mg/kg大蒜素的全價雞飼料,試驗周期為35d,每隔5天統(tǒng)計耗料量,隨時觀察雞群健康狀況。實驗結(jié)束時,每組取20只雞,稱量蛋雛雞體重、脾臟重、胸腺重及法氏囊重,計算免疫器官臟器系數(shù)、平均日增重和料重比。采用分光光度法、免疫濁度法、微量血凝抑制試驗、染色法、放射免疫測定技術及補體結(jié)合受體試驗檢測血清和免疫器官中超氧化物歧化酶(SOD)和谷胱甘肽過氧化物酶(GSH-Px)活性及丙二醛(MDA)含量;血清中免疫球蛋白G(Ig G)、免疫球蛋白M(Ig M)、免疫球蛋白A(Ig A)、補體C3及C4含量;血清新城疫抗體效價;血液中白細胞(WBC)、淋巴細胞及ANAE+T數(shù)量;血清白細胞介素2(IL-2)和腫瘤壞死因子α(TNF-α)含量;紅細胞表面檢測紅細胞受體花環(huán)率(RBC-C3b RR)和紅細胞免疫復合物花環(huán)率(RBC-ICR)。余下的120只實驗雞停止飼喂含大蒜素飼料,正常飼養(yǎng)至200日齡,檢測產(chǎn)蛋率、料蛋比、畸形蛋率。旨在探討大蒜素對蛋雞免疫功能及生產(chǎn)性能的影響,結(jié)果表明:(1)隨大蒜素劑量增加,大蒜素組蛋雛雞血清、脾臟、胸腺及法氏囊中SOD、GSH-Px活性顯著高于對照組(P0.05;P0.01),發(fā)蒜素組MDA含量顯著低于對照組(P0.05;P0.01),說明大蒜素能提升蛋雛雞抗氧化能力。(2)隨大蒜素劑量增加,大蒜素組蛋雛雞脾臟、胸腺及法氏囊的臟器系數(shù)均顯著高于對照組(P0.05;P0.01),說明大蒜素可促進蛋雛雞免疫器官生長發(fā)育。(3)隨大蒜素劑量增加,大蒜素組蛋雛雞血清Ig G、Ig A、Ig M含量及新城疫HI抗體效價均顯著高于對照組間(P0.05;P0.01),說明大蒜素可提高蛋雛雞體液免疫功能。(4)隨大蒜素劑量增加,大蒜素組蛋雛雞外周血中WBC數(shù)、淋巴細胞總數(shù)、ANAE陽性率均顯著高于對照組(P0.05;P0.01),說明大蒜素可提高蛋雛雞細胞免疫功能。(5)隨大蒜素劑量增加,大蒜素組蛋雛雞血清中補體C3、C4含量顯著高于對照組(P0.05;P0.01),說明大蒜素可提高蛋雛雞非特異性免疫功能。(6)隨大蒜素劑量增加,大蒜素組蛋雛雞血清中IL-2含量顯著高于對照組(P0.05;P0.01),大蒜素組TNF-α含量顯著低于對照組(P0.05;P0.01),說明大蒜素可通過調(diào)節(jié)細胞因子分泌增強蛋雛雞免疫功能。(7)隨大蒜素劑量增加,大蒜素組蛋雛雞紅細胞受體花環(huán)率顯著高于對照組(P0.05;P0.01),大蒜素組紅細胞免疫復合物花環(huán)率顯著低于對照組(P0.05;P0.01),說明大蒜素可提高蛋雛雞紅細胞免疫功能。(8)隨大蒜素劑量增加,大蒜素組蛋雛雞平均日增重顯著高于對照組(P0.05;P0.01),大蒜素組料重比顯著低于對照組(P0.05;P0.01),說明大蒜素可以促進蛋雛雞生長發(fā)育。(9)產(chǎn)蛋雞正常飼養(yǎng)到200日齡,大蒜素組蛋雞平均產(chǎn)蛋率顯著高于對照組(P0.05;P0.01),大蒜素組料蛋比及畸形蛋率顯著低于對照組(P0.05;P0.01),說明大蒜素能提高蛋雞生產(chǎn)性能,添加劑量為150mg/kg時效果最佳。
[Abstract]:In this experiment, Harbin city some 200 farms of 1 day old Hyline Brown female chicken as the research object, a week after the pre feeding, were randomly divided into 4 groups, each group of 50 chicks were fed diets containing 0 (control group), 50 (low dose group), 100 (middle dose group) and 150 (the high dose group) the full price of chicken feed mg /kg allicin, the test cycle is 35d, every 5 days to observe the statistics of material consumption, the health status of chickens. At the end of the experiment, each group of 20 chickens, chicken weighing weight, spleen weight, thymus weight and weight of bursa, immune organ coefficient calculation, the average daily gain and feed weight ratio. By spectrophotometry, turbidimetry, staining method of micro hemagglutination inhibition test, radioimmunoassay and enzyme receptor complement fixation test for the detection of serum and immune organs in superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity and malondialdehyde (MDA) content immune serum; 鐞冭泲鐧紾(Ig G),鍏嶇柅鐞冭泲鐧組(Ig M),鍏嶇柅鐞冭泲鐧紸(Ig A),琛ヤ綋C3鍙奀4鍚噺;琛，

本文編號：1635382