本文選題：仔豬　切入點：腹瀉　出處：《南京農(nóng)業(yè)大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：仔豬腹瀉是當(dāng)前導(dǎo)致仔豬死亡的重要原因之一,對仔豬的胃腸道發(fā)育有重要影響。微生態(tài)制劑作為抗生素替代品,已開始應(yīng)用于預(yù)防和控制仔豬腹瀉,但其具體使用效果和劑量還需要進(jìn)一步研究。本文研究了腹瀉仔豬心、肝、脾、腎等臟器指數(shù),小腸形態(tài),胃、小腸和大腸內(nèi)容物pH值等指標(biāo)的特征及灌服糞腸球菌微生態(tài)制劑對其影響,試圖分析并揭示微生態(tài)制劑對仔豬、尤某是對腹瀉仔豬生長發(fā)育的影響,為未來微生態(tài)制劑的應(yīng)用與推廣提供理論基礎(chǔ)。本試驗選用48窩仔豬,分為兩個階段進(jìn)行。第一階段(仔豬出生3-7天內(nèi))首先在被選48窩仔豬群中進(jìn)行,試驗前期細(xì)心觀察仔豬群健康狀態(tài)及腹瀉狀況,在仔豬出生3-7天內(nèi),一旦發(fā)現(xiàn)某一窩某一仔豬出現(xiàn)腹瀉情況,立即將該腹瀉仔豬及其同窩同性別的、未腹瀉的健康仔豬1頭進(jìn)行稱重、屠宰、樣品采集,直至采集完20窩仔豬的40個個體作為實驗組;第二階段(在仔豬出生8-20天內(nèi))是將這20窩實驗仔豬繼續(xù)飼養(yǎng)至第20天,即斷奶前一天,按照發(fā)生腹瀉日期相近、產(chǎn)期相近、產(chǎn)仔數(shù)相近的原則,將仔豬分成5個組,每組4個重復(fù)。其中C組為對照組,其余4組為試驗組,對照組于仔豬腹瀉當(dāng)日、第3日和第6日全窩灌服雙蒸水1 mL,對應(yīng)試驗T1組-T4組分別于對應(yīng)時間全窩依次灌服不同濃度的微生態(tài)制劑1 mL,濃度分別為2.5 ×108 CFU/mL、5 × 108 CFU/mL、1 × 109CFU/mL 和2 × 109 CFU/mL,結(jié)論如下:一、在仔豬出生3-7天內(nèi),腹瀉仔豬與未腹瀉仔豬相比,心、肝、脾、腎重量及其臟器指數(shù)均無顯著差異,除結(jié)腸pH有顯著差異外,胃、小腸及其余大腸pH值無差異,腹瀉仔豬組的十二指腸、空腸的絨毛高度和十二指腸絨腺比(絨毛高度/隱窩深度)均顯著低于未腹瀉仔豬組(P0.05),其它小腸形態(tài)無差異。二、灌服微生態(tài)制劑對仔豬脾臟指數(shù)和肺臟指數(shù)有顯著影響(P0.01),其中處理組1(T1)、處理組3(T3)、處理組4(T4)脾臟指數(shù)顯著(P0.05)高于對照組;T4組(P0.05)肺臟指數(shù)顯著高于對照組。灌服微生態(tài)制劑有影響仔豬肝臟指數(shù)的趨勢(P0.1),其中處理組1(T1)有低于對照組的趨勢(P0.1)。腹瀉及腹瀉與處理的交互作用對心臟指數(shù)、肝臟指數(shù)、脾臟指數(shù)、腎臟指數(shù)、肺臟指數(shù)均無影響。處理對回腸的pH值有顯著影響(P0.05),其中,T2、T3、T4組回腸pH值顯著低于(P0.05)對照組,T3、T4組回腸pH值也顯著低于T1組(P0.05)。除T3、T4組仔豬空腸絨毛高度顯著(P0.05)高于對照組外,處理、腹瀉以及腹瀉與處理的交互作用對仔豬小腸絨毛高度與隱窩深度及絨毛高度與隱窩深度比均無顯著影響(P0.05)。
[Abstract]:Diarrhea in piglets is one of the most important causes of piglet mortality, which has an important effect on the gastrointestinal development of piglets. As a substitute for antibiotics, microecological agents have been used to prevent and control diarrhea in piglets. However, further study is needed on the effect and dosage of the medicine. The indexes of heart, liver, spleen and kidney, the shape of small intestine, the stomach of piglets with diarrhea were studied in this paper. The characteristics of pH value of intestinal and large intestine contents and the effects of enterococcus faecalis microecological preparation on the growth and development of piglets, especially diarrhea piglets, were analyzed and revealed. In this experiment, 48 piglets were selected and divided into two stages. The first stage (within 3-7 days of birth) was first carried out in the selected 48 piglets. In the early stage of the experiment, we carefully observed the state of health and diarrhea of the piglets. Within 3 to 7 days after the piglets were born, once a certain piglet was found to have diarrhea, the diarrhea piglets and the same litter of the piglets of the same sex were immediately observed. One healthy piglet without diarrhea was weighed, slaughtered, and samples were collected until 40 individuals from 20 piglets were collected as experimental groups; the second stage (within 8-20 days of birth) continued to feed the 20 experimental piglets until the 20th day. On the day before weaning, piglets were divided into five groups according to the principle of similar diarrhea date, similar birth time and similar litter size. Group C was the control group, the other four groups were the experimental group, and the control group was on the day of piglet diarrhea. On 3rd and 6th, T4 group T _ 1 was fed with different concentrations of microecological agents (2.5 脳 10 ~ 8 CFU / m ~ (L) ~ (5 脳 10 ~ 8) CFU / m ~ (-1)) and 2 脳 10 ~ (9) CFU / mL respectively. The results are as follows: 1. There was no significant difference in heart, liver, spleen, kidney weight and viscera index between diarrhea piglets and non-diarrhea piglets in 3-7 days after birth. Except for colonic pH, there was no significant difference in pH value in stomach, small intestine and other large intestine. The villi height of duodenum and jejunum and the ratio of villus height to crypt in duodenum of diarrhea piglets were significantly lower than those in piglets without diarrhea (P 0.05), but there was no difference in other intestinal morphology. The spleen index and lung index of piglets treated with microecological preparation were significantly higher than those of control group (P 0.01). The spleen index of treatment group was significantly higher than that of control group (P 0.05), and that of treatment group was significantly higher than that of control group (P 0.05), and that of treatment group was significantly higher than that of control group (P 0.05), and that of treatment group was significantly higher than that of control group (P < 0.05), and that of treatment group was significantly higher than that of control group (P < 0.05). There was a tendency to affect liver index of piglets in the state preparation, in which the treatment group (1) was lower than that in the control group (P _ (0.1)). Diarrhea and the interaction between diarrhea and treatment had a significant effect on the cardiac index. Liver index, spleen index, kidney index, There was no effect of lung index on the pH value of ileum, and the pH value of ileum in T2T3T3T4 group was significantly lower than that in P0.05 group (P 0.05). The pH value of ileum in T3T4 group was significantly lower than that in T1 group (P0.05), and the villus height of jejunum in T3T4 group was significantly higher than that in control group (P0.05), and the pH value of jejunum villi in T3 T4 group was significantly lower than that in control group (P 0.05). Diarrhea and the interaction between diarrhea and treatment had no significant effect on the villi height and crypt depth and the ratio of villi height to crypt depth in piglets.
【學(xué)位授予單位】：南京農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S858.28

【參考文獻(xiàn)】

中國期刊全文數(shù)據(jù)庫 前10條

1 周盟;張乃鋒;董曉麗;王黎文;屠焰;紀(jì)守坤;張立霞;崔祥;樓燦;刁其玉;;益生菌對斷奶仔豬生長性能、免疫器官指數(shù)及胃腸道pH的影響[J];動物營養(yǎng)學(xué)報;2014年02期

2 王東衛(wèi);葛慎鋒;王明弟;;腐植酸基微生態(tài)制劑治療仔豬腹瀉效果初步觀察[J];腐植酸;2013年03期

3 辛娜;張乃鋒;刁其玉;周盟;;芽孢桿菌制劑對斷奶仔豬生長性能、胃腸道發(fā)育的影響[J];畜牧獸醫(yī)學(xué)報;2012年06期

4 高存川;徐春厚;;微生態(tài)制劑在水產(chǎn)養(yǎng)殖水質(zhì)改良中的應(yīng)用[J];湖北農(nóng)業(yè)科學(xué);2012年07期

5 楊靜利;張莉;;微生態(tài)制劑研究進(jìn)展[J];滄州師范學(xué)院學(xué)報;2012年01期

6 張慶麗;張志剛;;乳仔豬營養(yǎng)及消化生理研究進(jìn)展[J];飼料廣角;2010年23期

7 邸靜;郭迎春;楊英;;合生元對動物腸道微生態(tài)系統(tǒng)影響的研究進(jìn)展[J];黑龍江畜牧獸醫(yī);2010年23期

8 潘康成;吳敏峰;張世新;唐榮;;動物微生態(tài)理論與低碳畜牧業(yè)的可持續(xù)發(fā)展[J];中國家禽;2010年18期

9 唐毅敏;;微生態(tài)學(xué)與微生態(tài)制劑的研究進(jìn)展[J];全科護(hù)理;2008年31期

10 郝光;王振國;王雪飛;楊英;;合生元的研究進(jìn)展[J];中國微生態(tài)學(xué)雜志;2008年05期

中國博士學(xué)位論文全文數(shù)據(jù)庫 前2條

1 劉金海;黑曲霉菌在生物發(fā)酵飼料上的應(yīng)用及其產(chǎn)品對動物抗病力的影響[D];吉林大學(xué);2012年

2 蘇勇;16S rRNA基因分子技術(shù)研究仔豬胃腸道菌群區(qū)系變化[D];南京農(nóng)業(yè)大學(xué);2007年

中國碩士學(xué)位論文全文數(shù)據(jù)庫 前4條

1 房正國;不同脂肪來源的日糧對仔豬腸道發(fā)育及腸道微生物區(qū)系的影響[D];南京農(nóng)業(yè)大學(xué);2012年

2 晏家友;緩釋復(fù)合酸化劑對斷奶仔豬消化道酸度及腸道形態(tài)和功能的影響[D];四川農(nóng)業(yè)大學(xué);2009年

3 邢廣林;甘露寡糖、中藥和微生態(tài)制劑對肉雞抗病性、消化酶活性及生產(chǎn)性能的影響[D];山東農(nóng)業(yè)大學(xué);2006年

4 王磊;不同品種豬早期胃腸道的發(fā)育及養(yǎng)分利用率差異的研究[D];四川農(nóng)業(yè)大學(xué);2006年

，

本文編號：1635164