發(fā)布時(shí)間：2018-03-18 03:34

  本文選題：犬細(xì)小病毒　切入點(diǎn)：全血輸入療法　出處：《南京農(nóng)業(yè)大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：隨著時(shí)代的發(fā)展,寵物儼然成為我們家庭中重要的一員,寵物的健康關(guān)系到家庭的和諧。而犬的細(xì)小病毒感染目前對于幼犬危害比較大,尤其是胃腸出血性的犬細(xì)小病毒更是因?yàn)槠浒l(fā)病過程中大量的失血及離子的丟失紊亂奪去了許多可愛鮮活的小生命。九十年代早期,國內(nèi)有一些學(xué)者和科學(xué)試驗(yàn)機(jī)構(gòu)將輸血療法應(yīng)用于寵物臨床,并取得了一定的成功,論文對犬血液在臨床醫(yī)療上的篩選配型及采集儲存作了詳細(xì)的闡述,并用實(shí)例證明了輸血療法在提高犬小病毒的臨床治愈率上的作用是很明顯的。研究內(nèi)容分為二個(gè)部分:1.犬血液在臨床醫(yī)療上的篩選配型及采集儲存在使用輸血療法救治病犬之前,需要對供血犬的品種和血型進(jìn)行選擇,要選擇做過免疫的犬作為供血犬,然后進(jìn)行血涂片、病毒、血液生化指標(biāo)、體內(nèi)及體表寄生蟲方面的檢測,檢測完畢后進(jìn)行犬血液交叉配血實(shí)驗(yàn)。分別從供體和受體采血1ml,并用EDTA抗凝,在離心機(jī)內(nèi)離心1min,然后把受體和供體的紅細(xì)胞分別配成2%紅細(xì)胞混懸液,用生理鹽水進(jìn)行清洗,最后分別將供血犬的紅細(xì)胞和受血者的血清,受血者的紅細(xì)胞和供血者的血清混合,觀察是否有凝聚反應(yīng)。犬全血的儲存主要指的是紅細(xì)胞的保存,犬全血的采集一般是現(xiàn)采現(xiàn)輸,通過在注射器里加入抗凝劑,在靜脈采血后加入生理鹽水或者糖鹽水后100ml～200ml搖勻。2.全血輸入療法在犬細(xì)小病毒病治療中的實(shí)際運(yùn)用國內(nèi)犬細(xì)小病毒感染發(fā)病率呈逐年遞增趨勢,且在防疫、診斷、治療等方面存在很多問題,通過對犬細(xì)小病毒感染病犬的調(diào)查發(fā)現(xiàn),全血小病毒的感染與犬齡、季節(jié)、品種和犬只是否免疫有關(guān),犬只患犬細(xì)小病毒病表現(xiàn)的臨床癥狀為:嘔吐、腹瀉、血便、精神狀態(tài)差、食欲低下等。可采用犬細(xì)小病毒膠體金快速診斷試紙針對犬的糞便、生理指標(biāo)和血?dú)膺M(jìn)行檢測和診斷,確診后通常采用注射細(xì)小病毒單克隆抗體或含細(xì)小病毒的高免血清治療。本文還對全血輸入法治療犬細(xì)小病毒病犬的實(shí)例進(jìn)行分析,實(shí)例中列舉了不同品種、不同年齡、不同臨床癥狀的犬只,分析發(fā)現(xiàn),犬細(xì)小病毒的全血輸入療法在治療犬出血性腸炎犬細(xì)小病毒病和多種病因結(jié)合的復(fù)雜性犬細(xì)小病毒病上具有很好的效果。
[Abstract]:With the development of the times, pets have become an important member of our family. The health of pets is related to the harmony of the family. However, the dog's parvovirus infection is now more harmful to puppies. In particular, gastrointestinal hemorrhagic canine parvovirus is the result of massive blood loss and ion loss disorders that have taken away many lovely, fresh, small lives in the early days of 90s. Some domestic scholars and scientific experimental organizations have applied transfusion therapy to pet clinic, and have achieved certain success. This paper expounds in detail the selection, matching, collection and storage of canine blood in clinical medicine. The effect of transfusion therapy on the clinical cure rate of canine small virus is proved to be very obvious. The research is divided into two parts: 1.The screening matching of canine blood in clinical medicine and the collection and storage in the use of blood transfusion therapy. Before the treatment of the sick dog, We need to select the breed and blood type of the blood supply dog, select the dog that has been immunized as the blood supplying dog, and then test the blood smear, virus, blood biochemical index, body surface parasite. The blood was collected from donor and recipient at 1ml, and then centrifuged in centrifuge for 1 min with EDTA. Then, the recipient and donor erythrocytes were mixed into 2% erythrocyte suspension respectively and washed with normal saline. Finally, the red blood cells of the dogs and the sera of the recipients, the red blood cells of the recipients and the sera of the donors were mixed to observe whether there was a condensation reaction. The storage of the whole blood of the dogs mainly refers to the preservation of the red blood cells. The collection of whole blood in dogs is generally collected and delivered, by adding an anticoagulant to the syringe. Application of whole blood transfusion therapy in the treatment of canine parvovirus infection the incidence of dog parvovirus infection is increasing year by year. There are many problems in treatment. Through the investigation of canine parvovirus infected dogs, it is found that the infection of whole blood virus is related to dog age, season, breed and whether the dog is immunized. The clinical symptoms of canine parvovirus disease are vomiting, diarrhea, blood stool, poor mental state, low appetite and so on. Physiological indexes and blood gas were detected and diagnosed. After diagnosis, monoclonal antibodies to parvovirus or high immune serum containing parvovirus were usually used to treat canine parvovirus disease, and the examples of whole blood transfusion in the treatment of canine parvovirus disease dogs were also analyzed in this paper. Examples of different breeds, different ages, different clinical symptoms of dogs, analysis found, The whole blood transfusion therapy of canine parvovirus has a good effect on the treatment of hemorrhagic enteritis canine parvovirus disease and complicated canine parvovirus disease with multiple etiological factors.
【學(xué)位授予單位】：南京農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S858.292

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