本文選題：禽戊型肝炎病毒　切入點：ELISA檢測　出處：《西北農(nóng)林科技大學》2016年碩士論文　論文類型：學位論文

【摘要】：禽戊型肝炎病毒(Hepatitis E virus,HEV)是雞的大肝大脾(Big liver and spleen disease,BLS)病或肝炎脾大(Hepatitis-Splenomegaly,HS)綜合癥的主要病原。該病主要引起30-72周齡的蛋雞和肉種雞的死淘率升高(1%-4%)和產(chǎn)蛋率下降(20%-40%),發(fā)病雞通常腹部充血,卵巢退化,肝臟脂肪或淀粉樣變性,偶有肝脾腫大。禽HEV感染曾給澳大利亞的養(yǎng)禽業(yè)造成了嚴重的經(jīng)濟損失,而我國也時有該病爆發(fā)的報道。2001年禽HEV被首次分離報道,隨后許多國家都有該病毒的分離報道。在我國,2010年從患有HS綜合征的病雞中分離得到禽HEV,血清學調(diào)查發(fā)現(xiàn)該病毒感染已在我國許多蛋雞場中普遍存在。本研究首先從楊凌的12個蛋雞場采集了3337份血清,然后利用已建立的禽HEV抗體檢測的間接ELISA方法檢測血清中的抗體。ELISA檢測結果發(fā)現(xiàn)1668份血清為禽HEV抗體陽性,陽性率為49.85%。隨后,依據(jù)2014-2015年陜西楊凌地區(qū)部分商品蛋雞場出現(xiàn)產(chǎn)蛋量下降(10%-40%),剖檢部分雞只發(fā)現(xiàn)肝臟腫大出血,疑似為禽HEV感染引起。隨后為確診該疫情的病因,本研究從發(fā)病的6個蛋雞場采集了268份糞便,利用巢式RT-PCR對糞便中的禽HEV RNA進行了實驗室檢測。巢式RT-PCR檢測結果發(fā)現(xiàn)63份糞便為禽HEV RNA基因片段陽性,陽性率為23.51%�；蚱蔚男蛄型葱苑治霭l(fā)現(xiàn),楊凌地區(qū)的禽HEV分離株與國內(nèi)的同源性為93.0-99.2%;進化樹分析表明,其與國內(nèi)其它地區(qū)的分離株在同一進化分支上,同屬禽HEV基因3型。
[Abstract]:Avian hepatitis e virus (HEV) is the main pathogen of the disease of the large liver and spleen disease of chickens or the hepatitis spleen Hepatitis-Splenomegalyus HSV syndrome. The disease mainly causes the increase of the death rate of 30-72 week old laying hens and broilers by 1-4) and the decrease of laying rate by 20% -40%, which is the main cause of disease, and the disease is the main cause of the disease, which causes the death rate of 30-72 week old laying hens and broilers to increase by 1-4) and the laying rate decreases by 20-40th. Chickens are usually congested in the abdomen, Ovarian degeneration, liver fat or amyloid degeneration, occasionally hepatosplenomegaly. Avian HEV infection has caused serious economic losses to the poultry industry in Australia, and outbreaks of the disease have been reported in China. In 2001, avian HEV was first isolated. In 2010, avian HEV was isolated from sick chickens with HS syndrome in China. Serological investigation showed that the virus infection has been widespread in many laying chicken farms in China. First, 3337 serum samples were collected from Yang Ling's 12 laying chicken farms. Then the indirect ELISA method for detection of avian HEV antibodies was used to detect antibodies in sera. Elisa results showed that 1 668 sera were positive for avian HEV antibodies, and the positive rate was 49.85%. According to the number of layers in some commercial laying farms in Yang Ling area of Shaanxi Province from 2014-2015 to 2014-2015, the amount of laying decreased by 10-40g. Some chickens were dissected and found liver swelling and bleeding, suspected to be caused by avian HEV infection. Subsequently, the etiology of the epidemic was confirmed. In this study, 268 feces were collected from 6 laying hens, and HEV RNA in feces was detected by nested RT-PCR. The results of nested RT-PCR analysis showed that 63 feces were positive for HEV RNA gene fragment. The positive rate was 23.51.The sequence homology analysis of the gene fragment showed that the homology of avian HEV isolates in Yang Ling area was 93.0-99.2, and the phylogenetic tree analysis showed that the isolates belonged to the same evolutionary branch of avian HEV gene as other isolates in China.
【學位授予單位】：西北農(nóng)林科技大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：S858.31

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