本文選題：WQB　切入點(diǎn)：免疫功能　出處：《河北農(nóng)業(yè)大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：中藥是傳統(tǒng)醫(yī)學(xué)的瑰寶。大量研究證實(shí),許多中藥具有增強(qiáng)機(jī)體免疫力、抗氧化、抗腫瘤的功效,本試驗(yàn)以衛(wèi)氣補(bǔ)(WQB)為研究對象,研究其對老年鼠的免疫及抗氧化功能的影響。通過急、慢性毒性試驗(yàn),研究WQB對青年鼠的安全性,為WQB的臨床應(yīng)用提供理論依據(jù)。研究中藥復(fù)方衛(wèi)氣補(bǔ)(WQB)對老年鼠免疫指標(biāo)及抗氧化功能的影響。50只12月齡的老年鼠,隨機(jī)分為5組,其中A組為對照組,B組飼喂10mg/kg BW左旋咪唑組,C、D、E組分別為0.1%、1%、2%WQB組,另取10只2月齡青年鼠作為F組,正常飼喂。分別按照上述分組飼喂30d。結(jié)果顯示,與對照組及左旋咪唑組相比,飼料中添加1%WQB能夠增加老年鼠體重及免疫器官肝、脾、胸腺指數(shù),顯著提高鼠體內(nèi)SOD以及IL-2水平并降低MDA含量(P0.05,P0.01);增加空腸粘膜固有層CD4+陽性細(xì)胞數(shù)量、抑制CD8+陽性細(xì)胞的表達(dá),增加十二指腸s Ig A細(xì)胞的表達(dá)量;飼料中添加1%、2%WQB均能顯著提高老年鼠血清中IFN-γ含量,并達(dá)到青年鼠水平。為評價WQB安全性,進(jìn)行WQB急性毒性試驗(yàn)研究。將80只2月齡青年鼠隨機(jī)分為7個劑量組分別為2 m L/kg BW、2.80 m L/kg BW、3.92 m L/kg BW、5.49 m L/kg BW、7.68 m L/kg BW、10.76 m L/kg BW、15.06 m L/kg BW及1個灌服生理鹽水的空白對照組,灌服1周后,各組小鼠均表現(xiàn)正常,沒有死亡情況,因此又進(jìn)行最大耐受劑量試驗(yàn)(MTD),灌服劑量為30.12 m L/kg BW,相當(dāng)于每只小鼠灌服0.9g原藥。飼喂1周后,小鼠表現(xiàn)正常,無死亡情況。取上述9組小鼠肝、腎器官稱重;由于在急性毒性試驗(yàn)期間,WQB各組小鼠沒有死亡,所以摘取15.06 m L/kg BW WQB組、空白對照組及MTD組小鼠肝、脾、肺、腎、十二指腸等器官,觀察其組織結(jié)構(gòu);眼眶采血,測定血常規(guī)。結(jié)果表明,各組小鼠肝、腎系數(shù)之間在0.05和0.01水平上無統(tǒng)計學(xué)意義;15.06 m L/kg BW WQB組及MTD組小鼠臟器組織結(jié)構(gòu)與空白對照組小鼠相比,結(jié)構(gòu)正常;血常規(guī)指標(biāo)如MID(中間細(xì)胞)、LYM(淋巴細(xì)胞)、RBC(紅細(xì)胞)、HGB(血紅蛋白)檢測結(jié)果,各組均無顯著性差異(P0.05)。為評價WQB安全性,進(jìn)一步進(jìn)行WQB慢性毒性試驗(yàn)研究。將15只2月齡青年鼠隨機(jī)分為3組,分別為空白對照組:正常飼喂;陽性對照組:飼喂10 mg/kg BW苯丙左旋咪唑;1%WQB組:在飼料中加拌1%WQB。飼喂90d后取材,摘取肝、腎組織并稱重;由于在慢性毒性試驗(yàn)期間,小鼠沒有死亡,所以選取空白對照組、1%WQB組小鼠肝、脾、肺、腎、十二指腸組織固定,用于觀察組織結(jié)構(gòu)。眼眶采血用于血生化指標(biāo)甘油三酯(TG)、膽固醇(CHOL)、葡萄糖(GLU)、尿酸(UA)、肌酐(Crea)、丙氨酸轉(zhuǎn)氨酶(ALT)、尿素氮(BUN)、總蛋白(TP)、白蛋白(ALB)、天冬氨酸轉(zhuǎn)氨酶(AST)的檢測。結(jié)果表明,各組小鼠生長良好,灌服1%WQB組小鼠免疫器官指數(shù)及體重變化與正常對照組及苯丙左旋咪唑組相比,無顯著性差異;1%WQB組小鼠與正常對照組小鼠臟器組織結(jié)構(gòu)相比,無變化;1%WQB組小鼠血生化指標(biāo)與空白對照組和苯丙左旋咪唑組小鼠相比,差異不顯著(P0.05)。本試驗(yàn)表明飼料中加入1%WQB可提高老年鼠免疫力及抗氧化能力,且安全無毒。
[Abstract]:Chinese medicine is a treasure of traditional medicine. A number of studies have demonstrated that many Chinese medicine has to enhance immunity, anti-oxidation, anti-tumor effect, the Wei Qi supplement (WQB) as the research object, to study the influence of old mice's immune function and antioxidation. The acute, chronic toxicity test, safety study of WQB in young rats, and provide a theoretical basis for the clinical application of WQB. The study of compound traditional Chinese medicine Qi supplement (WQB) effect of.50 on immune function and antioxidant function in aged rats only 12 month old aged rats, were randomly divided into 5 groups, group A as control group, B group fed with 10mg/kg BW levomisole group, C. D, E group were 0.1%, 1%, 2%WQB group, another 10 male 2 month old young rats as F group, normal feeding. The feeding group respectively according to the 30D. results showed that compared with the control group and levamisole group, 1%WQB supplementation can increase the weight of immune organs and liver spleen in aged rats, Thymus index increased significantly in rats SOD and the level of IL-2 and decrease the content of MDA (P0.05, P0.01); increase the number of CD4+ positive cells in jejunum mucosa, inhibit the expression of CD8+ positive cells, increase the expression of s Ig A cells in the duodenum; add 1% feed, 2%WQB could significantly increase the IFN- content of serum gamma in aged rats in young rats and reached the level. In order to evaluate the safety of WQB, experimental study on acute toxicity of WQB. 80 2 month old young rats were randomly divided into 7 groups respectively for 2 m L/kg BW m L/kg, 2.80 BW, 3.92 m L/kg BW m L/kg, 5.49 BW, 7.68 m L/kg BW 10.76, m L/kg BW m L/kg, 15.06 BW and 1 normal saline control group, gavage after 1 weeks, the mice showed normal, no death case, and thus the maximum tolerated dose test (MTD), dose 30.12 m L/kg BW, the equivalent of every mice fed with 0.9g TC. Feeding After 1 weeks, the mice appeared normal and no death. The 9 groups of mice liver, kidney organ weighing; during the period of acute toxicity test, WQB mice without death, so the removal of 15.06 m L/kg BW WQB group, blank control group and MTD group of mice liver, spleen, lung, kidney, intestine, twelve the organ, to observe the structure; blood, determination of blood. The results showed that the mice liver, kidney coefficient between 0.05 and 0.01 levels was not statistically significant; 15.06 m L/kg BW WQB group and MTD group of mice organ structure and the blank control group were compared with normal structure; blood routine indexes such as MID (intermediate cell) LYM, RBC (lymphocytes), (RBC), HGB (hemoglobin) test results, there were no significant differences between the groups (P0.05). To evaluate the safety of WQB, further experimental study of WQB chronic toxicity. 15 2 month old young rats were randomly divided into 3 groups, namely control group: Normal feeding; positive control group: rats were fed with 10 mg/kg BW styrene acrylate levamisole; group 1%WQB: 1%WQB. add mix feeding 90d in feed harvested after removal of the liver, kidney and weighed; because during the chronic toxicity test, mice without death, so select the blank control group, 1%WQB group of mice liver, spleen and lung. Kidney, duodenal tissue fixed for histological observation. Blood biochemical index for triglyceride (TG), cholesterol (CHOL), glucose (GLU), uric acid (UA), creatinine (Crea), alanine aminotransferase (ALT), urea nitrogen (BUN), total protein (TP), albumin (ALB), aspartate aminotransferase (AST) detection. The results showed that the mice fed with good growth, immune organ index and body weight in 1%WQB group compared with the normal control group and phenylpropanoid levamisole group, no significant difference; in 1%WQB group compared with the normal control group mice. No change; 1 Compared with blank control group and phenylpropanolone group, there was no significant difference in blood biochemical index between%WQB group and P0.05 group (P0.05).

【學(xué)位授予單位】：河北農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S853.7

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