本文選題：奶牛　切入點：排卵延遲　出處：《石河子大學》2015年碩士論文　論文類型：學位論文

【摘要】：目的:奶牛排卵延遲是規(guī)�；膛龅乃拇舐殉布膊≈�。本研究立足于生產實踐中的實際問題:奶牛場排卵延遲診斷錯誤、不準確、治療沒有針對性,以及沒有配種后保胎等共性問題,深入系統(tǒng)的對奶牛發(fā)情到排卵和排卵直至黃體形成期間的激素、部分細胞因子的變化規(guī)律進行了檢測分析,以期為建立奶牛排卵延遲的診斷和配種后保胎提供依據。在此基礎上,對排卵延遲奶牛進行了優(yōu)化方案的治療。以上最終目的是為了建立奶牛排卵延遲診斷程序、方法和優(yōu)化治療和保胎方案,提高受胎率,從而減少奶牛的經濟損失。方法:從西部牧業(yè)中心奶牛場選取排卵延遲奶牛12頭,臨床健康正常發(fā)情排卵奶牛20頭,從發(fā)情到配種后15天,連續(xù)跟蹤采血,應用ELISA方法檢測采集血清中的P4、E2、LH、FSH和IGF-I濃度,其中排卵延遲組又分為兩組,分別為輸精時注射LHRH-A3(定為排卵延遲1組)和輸精時不注射LHRH-A3(定為排卵延遲2組),分析其激素變化規(guī)律。同時應用50s型Tringa Vet便攜式線陣B超儀在配種后7-15天進行跟蹤掃查(隔2天1次),用B超儀內置的電子尺測量了黃體周長、黃體面積、黃體體積以及黃體直徑,統(tǒng)計分析這些黃體指標的變化規(guī)律。依據以上研究,制定了奶牛排卵延遲的診斷方法和保胎方案,然后在奶牛場對常規(guī)激素治療方案(LHRH-A3)、中藥治療組(理囊散)和優(yōu)化治療方案選擇了60頭排卵延遲奶牛進行了臨床療效觀察。結果:第2天上午排卵延遲1組(注射LHRH-A3)血清LH濃度低于正常組奶牛且差異顯著(P0.05),其它各個階段差異均不顯著(P0.05);排卵延遲1組(注射LHRH-A3)與排卵延遲2組(未注射LHRH-A3)相比,奶牛血清中的P4、E2、LH、FSH和IGF-I的濃度在各個階段均差異不顯著(P0.05);正常組與排卵延遲2組(未注射LHRH-A3)相比,奶牛血清中的P4、E2、LH、FSH和IGF-I的濃度在各個階段均差異不顯著(P0.05),正常組奶牛的血清P4濃度在所有階段均高于排卵延遲2組(未注射LHRH-A3),但差異不顯著(P0.05)。通過對12頭臨床健康奶牛和20頭排卵延遲奶牛進行B超掃查,獲得了正常健康和排卵延遲奶牛黃體形成過程中不同階段的黃體典型聲像圖,正常組與卵延遲組奶牛相比,黃體相關指標(直徑、周長、面積和體積)在配種后7-15天內的變化規(guī)律,差異不顯著,(P0.05)。應用優(yōu)化方案治療效果最好,治愈率為70%,其次為中藥理囊,治愈率為50%,常規(guī)激素LHRH-A3治療效果最差,治愈率為37.5%。結論:通過對排卵延遲奶牛從發(fā)情到配種后黃體形成過程中的激素(P4、E2、LH、FSH)、細胞因子(IGF-I)、黃體周長、黃體面積、黃體體積以及黃體直徑指標變化規(guī)律系統(tǒng)的研究,為建立奶牛排卵延遲的診斷方法奠定了基礎。應用優(yōu)化方案治療奶牛排卵延遲比常規(guī)激素LHRH-A3治療方案、中藥理囊臨床效果更好,優(yōu)化治療方案是排卵延遲奶牛在配種同時注射LHRH-A325μg,在配種后5-7天、15-17天分別肌肉注射長效黃體酮注射液100mg,在配種后28天肌肉注射VADE10ml進行保胎,可以提高受胎率。
[Abstract]:Objective: cows delayed ovulation is one of the large-scale dairy farms in four ovarian disease. This research is based on the actual problems in production practice: Dairy delayed ovulation is not accurate, error diagnosis, treatment is not targeted, and no problem of common miscarriage after mating, thorough the oestrus and ovulation until ovulation during the formation of corpus luteum hormone changes of some cytokines were detected and analyzed, in order to provide the basis for the establishment of diagnosis and miscarriage after delayed ovulation cows breeding. On this basis, the delayed ovulation cows were treated more than the optimal solution. The final purpose is to establish the cow delayed ovulation diagnostic procedures, treatment methods and optimization and improve the pregnancy rate, miscarriage plan, so as to reduce the economic losses of dairy cows. Methods: selected delayed ovulation 12 cows from western animal husbandry dairy clinical center, healthy hair Love ovulation 20 cows, from estrus to 15 days after mating, continuous tracking sampling, ELISA method was used to detect serum collected in P4, E2, LH, FSH and IGF-I concentration, which delayed ovulation group was divided into two groups, respectively, using LHRH-A3 injection (as delayed ovulation group 1) and transmission precision when the injection of LHRH-A3 (as delayed ovulation group 2), analyze the changes of hormone. At the same time in the array B-scanner 7-15 days after mating tracking scan using 50s Tringa Vet portable (2 days every 1 lines), corpus luteum circumference, were measured by electronic scale B-scanner built-in luteal area, luteal volume and the diameter, statistical analysis the variation of corpus luteum index. According to the above study, the diagnostic method of cow delayed ovulation and miscarriage plan, then at the dairy of conventional hormone therapy (LHRH-A3), Chinese medicine treatment group (cyst regulating powder and optimize the treatment plan) chose 60 head of ovulation The cows were delayed clinical observation. Results: the morning of the second day delayed ovulation group 1 (with LHRH-A3) serum LH concentration is lower than the normal group of dairy cows (P0.05), and the difference was significant difference in other stages were not significant (P0.05); the 1 group delayed ovulation (injection of LHRH-A3) and delayed ovulation group 2 (no injection LHRH-A3) compared to cow serum P4, E2, LH, FSH and IGF-I concentrations in each stage were not significant (P0.05); the normal group and delayed ovulation group 2 (without LHRH-A3) than in cow serum P4, E2, LH, FSH and IGF-I concentrations in each stage were not significant (P0.05), serum concentration of P4 group was higher than that of normal cows delayed ovulation 2 groups at all stages (without LHRH-A3), but the difference was not significant (P0.05). Ultrasound scanning in 12 clinical healthy cows and 20 delayed ovulation cows, got healthy and delayed ovulation cows luteal formation process The typical sonographic luteal phase in different stages, the normal group and delayed group compared to cow eggs, relevant indicators of corpus luteum (diameter, perimeter, area and volume) changes in the 7-15 days after mating period, no significant difference (P0.05). Application of optimization scheme is the best treatment, the cure rate was 70%, followed by traditional Chinese medicine science capsule, the cure rate was 50%, conventional hormone therapy effect of LHRH-A3 is the worst, the cure rate was 37.5%. conclusion: the delayed ovulation cows from estrus to mating in the process of formation of corpus luteum hormone (P4, E2, LH, FSH), cell factor (IGF-I), yellow body circumference, corpus luteum area, changes of system the diameter of luteal volume and luteal index, laid the foundation for the establishment of diagnosis method of cows delayed ovulation. Ovulation delay than the conventional hormone LHRH-A3 treatment regimen in the treatment of traditional Chinese medicine application optimization of cows, physical capsule has better clinical efficacy, optimization of treatment is delayed ovulation in milk At the same time in the breeding cows injected with G LHRH-A325, in 5-7 days after mating, 15-17 days respectively, intramuscular injection of long-acting Progesterone Injection 100mg in 28 days after mating, intramuscular injection of VADE10ml can improve the pregnancy rate of miscarriage,.

【學位授予單位】：石河子大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S858.23

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