本文關(guān)鍵詞： 胎兒成纖維細(xì)胞 成體干細(xì)胞 多能性 增殖 凋亡　出處：《基因組學(xué)與應(yīng)用生物學(xué)》2017年02期 　論文類型：期刊論文

【摘要】：本研究旨在探索阿爾巴斯白絨山羊成體干細(xì)胞和胎兒成纖維細(xì)胞體外生長、增殖能力和胚胎干細(xì)胞特性的差異,為成體干細(xì)胞的應(yīng)用提供新的理論基礎(chǔ)。研究以胎兒成纖維細(xì)胞(fetal fibroblast cells,FFCs)作為對照,檢測阿爾巴斯白絨山羊3種成體干細(xì)胞,脂肪間充質(zhì)干細(xì)胞(adipose-derived stem cells,ADSCs)、骨髓間充質(zhì)干細(xì)胞(bone marrow stromal cells,BMSCs)和肌肉衛(wèi)星細(xì)胞(muscle-derived satellite cells,MDSCs)中多能性(NANOG,OCT4,SOX2)、增殖(TERT,PCNA)、凋亡(P53,BAX)基因的轉(zhuǎn)錄和表達(dá)。表明g ADSCs、g BMSCs和g MDSCs低表達(dá)NANOG、OCT4和SOX2,與g FFCs沒有明顯差異;TERT在g BMSCs和g MDSCs中的表達(dá)量高于g FFCs中的表達(dá)量,而在g ADSCs中的表達(dá)量則低于g FFCs;PCNA和BAX在g FFCs和g MDSCs中高表達(dá),在g ADSCs和g BMSCs中低表達(dá);P53在g BMSCs中的相對表達(dá)量較高,在g FFCs、g ADSCs和g MDSCs中的相對表達(dá)量均較低。現(xiàn)象表明,在一定程度上成體干細(xì)胞的胚胎干細(xì)胞特性及增殖能力優(yōu)于胎兒成纖維細(xì)胞。
[Abstract]:The aim of this study was to explore the difference between adult stem cells and fetal fibroblasts in vitro growth, proliferation and characteristics of embryonic stem cells. To provide a new theoretical basis for the application of adult stem cells, fetal fibroblasts (FFCs) were used as control. Three kinds of adult stem cells and adipose-derived stem cells were detected. Bone marrow stromal cells. BMSCs) and muscle-derived satellite cells (MDSCs). The transcription and expression of SOX2, ADSCs, P53 and Bax) genes showed that g ADSCs. G BMSCs and g MDSCs were not significantly different from g FFCs in the expression of NANOGN OCT4 and SOX2. The expression of TERT in g BMSCs and g MDSCs was higher than that in g FFCs, but the expression in g ADSCs was lower than that in g FFCs. The expression of PCNA and BAX was high in g FFCs and g MDSCs, and low in g ADSCs and g BMSCs. The relative expression of p53 in g BMSCs was higher than that in g FFCsG ADSCs and g MDSCs. To a certain extent, the embryonic stem cells of adult stem cells are superior to fetal fibroblasts in the characteristics and proliferation of embryonic stem cells.
【作者單位】： 內(nèi)蒙古大學(xué)哺乳動物生殖生物學(xué)及生物技術(shù)教育部重點(diǎn)實(shí)驗(yàn)室;
【基金】：高產(chǎn)絨量轉(zhuǎn)基因絨山羊新品種培育項(xiàng)目(2014ZX08008-002)資助
【分類號】：S827
【正文快照】： 阿爾巴斯白絨山羊在內(nèi)蒙古地區(qū)具有很高的經(jīng)1.2免疫細(xì)胞熒光檢測多能性增殖和凋亡相關(guān)基因濟(jì)價(jià)值,其轉(zhuǎn)基因新品種的培育及大規(guī)模繁殖不僅的表達(dá)對內(nèi)蒙古自治區(qū)的經(jīng)濟(jì)發(fā)展具有很大的影響,同時(shí)免疫細(xì)胞熒光檢測顯示,gF FCs中幾乎檢測不到對當(dāng)代畜牧業(yè)的發(fā)展也具有重大意義。目前，

本文編號：1478093