本文關鍵詞： 斷奶仔豬 糞腸球菌 抗生素 生長性能 腹瀉 血液生化指標 小腸形態(tài) 盲腸菌群　出處：《西南大學》2015年碩士論文　論文類型：學位論文

【摘要】：本試驗在斷奶仔豬飼糧中添加不同水平的糞腸球菌,研究其對斷奶仔豬生長性能、腹瀉程度、血液生化指標、抗氧化能力、免疫器官指數(shù)、小腸形態(tài)發(fā)育以及盲腸菌群的影響。選取120頭(28±2)d平均體重為(7.60±0.81)kg的“杜×長×大”斷奶仔豬,隨機分為5組,每組6個重復(公母各半),每個重復4頭,每個重復為一圈。對照組飼喂基礎飼糧,抗生素組在基礎飼糧中添加100 mg/kg的硫酸粘菌素和400 mg/kg的桿菌肽鋅,三個糞腸球菌組分別在基礎飼糧中添加40、200和1000 mg/kg的糞腸球菌。試驗期31天。試驗結果顯示：1)在1-14d,飼糧中添加抗生素和糞腸球菌對斷奶仔豬的平均日采食量(ADFI)、平均日增重(ADG)以及料重比(F/G)無顯著影響(P0.05)。在15-31d,與對照組和抗生素組相比,糞腸球菌200和1000 mg/kg組可極顯著提高ADFI和ADG(P0.01)。在1-31d,與對照組相比,糞腸球菌200和1000 mg/kg組的ADFI均極顯著提高(P0.01),而ADG分別顯著(P0.05)和極顯著(P0.01)增加；與抗生素組相比,糞腸球菌1000 mg/kg組可極顯著提高ADFI(P0.01),而糞腸球菌200和1000 mg/kg組的ADG分別顯著(P0.05)和極顯著(P0.01)增加。2)在1-14d,與對照組相比,糞腸球菌40 mg/kg組的腹瀉指數(shù)和腹瀉率顯著降低(P0.05)。在15-31d和1-31d,與對照組和抗生素組相比,糞腸球菌40和1000 mg/kg組均可在一定程度上降低腹瀉率(P0.05)。3)與對照組相比,糞腸球菌可以顯著提高血液中總蛋白含量,其中糞腸球菌200和1000 mg/kg組達到極顯著水平(P0.01),且這兩組分別可以顯著(P0.05)和極顯著(P0.01)提高球蛋白含量。與抗生素組相比,糞腸球菌200和1000 mg/kg組可以顯著提高總蛋白含量(P0.05)。與對照組相比,抗生素組和糞腸球菌1000 mg/kg組的白蛋白與球蛋白含量之比(白球比)顯著降低(P0.05),另外,抗生素組血液中谷丙轉氨酶和谷草轉氨酶活性顯著降低(P0.05),糞腸球菌200 mg/kg組的谷丙轉氨酶活性顯著降低(P0.05)。4)與對照組相比,飼糧中添加糞腸球菌對斷奶仔豬血清中丙二醛(MDA)含量影響不顯著(P0.05),而抗生素可顯著降低血清中MDA含量(P0.05)。糞腸球菌1000 mg/kg組可顯著提高谷胱甘肽過氧化物酶(GSH-PX)活性(P0.05),具有提高過氧化氫酶(CAT)活性的趨勢(P0.05)；200 mg/kg組可顯著提高CAT活性(P0.05),具有提高GSH-PX活性的趨勢(P0.05)；40mg/kg組可顯著提高CAT活性(P0.05)。與對照組和抗生素組相比,糞腸球菌1000 mg/kg組可以極顯著提高血清中超氧化物歧化酶(SOD)活性(P0.01),糞腸球菌200 mg/kg組具有提高SOD活性的趨勢(P0.05)。5)與對照組相比,糞腸球菌1000 mg/kg組的肝臟重量和脾臟指數(shù)有提高的趨勢(P0.05),與抗生素組相比,糞腸球菌200和1000 mg/kg組可顯著提高肝臟重量(P0.05)。6)與對照組相比,飼糧中添加1000 mg/kg的糞腸球菌可顯著提高回腸絨毛高度與隱窩深度比(V/C)(P0.05),并且具有提高十二指腸絨毛高度和降低回腸隱窩深度的趨勢(P0.05)；與抗生素組相比,飼糧中添加1000 mg/kg的糞腸球菌可顯著提高回腸V/C(P0.05),并且具有降低回腸隱窩深度的趨勢(P0.05)。7)飼糧中添加抗生素和糞腸球菌對斷奶仔豬盲腸菌群的有效序列數(shù)、操作分類單元(OTU)數(shù)以及a多樣性指數(shù)(Chao1指數(shù)、Shannon指數(shù)、Ace指數(shù)和Simpson指數(shù))均無顯著影響(P0.05)。與對照組相比,抗生素可分別在一定程度上提高和降低變形菌門和放線菌門細菌的比例(P0.05),糞腸球菌40和1000mg/kg組可在一定程度上降低變形菌門細菌的比例(P0.05)。與抗生素組相比,飼糧中添加1000 mg/kg的糞腸球菌有提高厚壁菌門細菌比例和降低變形菌門細菌比例的趨勢(P.05),糞腸球菌40 mg/kg組具有降低變形菌門細菌的趨勢(P0.05)。進一步從科的水平上分析盲腸菌群構成可知,與對照組相比,飼糧中添加1000 mg/kg的糞腸球菌可顯著增加斷奶仔豬盲腸中芽孢桿菌科細菌的比例(P0.05),顯著降低梭菌科和鏈球菌科細菌的比例(P0.05),具有增加乳酸桿菌科細菌的趨勢(P0.05)。糞腸球菌40 mg/kg組具有增加芽孢桿菌科細菌和降低梭菌科細菌的趨勢(P0.05)。與對照組相比,抗生素可在一定程度上增加腸桿菌科細菌比例(P0.05),而糞腸球菌可在一定程度上降低腸桿菌科細菌的比例(P0.05),且糞腸球菌組和抗生素組差異顯著(P0.05)。結果表明,飼糧中添加糞腸球菌可改善斷奶仔豬的生長性能(第15-31d),降低腹瀉率,提高斷奶仔豬的免疫力和抗氧化能力,促進其小腸形態(tài)發(fā)育,優(yōu)化盲腸菌群構成；在糞腸球菌的三個添加水平中,以添加量為1000 mg/kg的效果為最好。
[Abstract]:The experiment of adding different levels of Enterococcus faecalis in weaned piglets and study the growth performance of piglet diarrhea, blood biochemical index, antioxidant ability, immune organ index, effects of intestinal morphological development and cecal flora. Select 120 head (28 + 2) d average weight (7.60 + 0.81) kg "Duroc * Landrace * Large weaned piglets were randomly divided into 5 groups, with 6 replicates (male and female), each with 4 heads, each repeat is a circle. The control group was fed the basal diet, bacitracin zinc sulfate stick antibiotics group add 100 mg/kg in the basic diet in 400 and mg/kg the three group of Enterococcus faecalis were added 40200 and 1000 mg/kg in the basic diet of Enterococcus faecalis. The trial period was 31 days. The results showed: 1) in 1-14d, the addition of antibiotics and Enterococcus faecalis in diet of weaned piglets on average daily feed intake (ADFI), average daily gain (ADG) and Feed weight ratio (F/G) had no significant effect (P0.05). In 15-31d, compared with control group and antibiotics group, 200 Enterococcus faecalis and 1000 mg/kg groups could significantly increase ADFI and ADG (P0.01). In 1-31d, compared with the control group, 200 Enterococcus faecalis and 1000 mg/kg groups increased significantly (ADFI P0.01), and ADG (P0.05) were significantly and very significantly increased (P0.01); compared with the antibiotic group, 1000 Enterococcus faecalis mg/kg group could significantly increase the ADFI (P0.01), and Enterococcus faecalis in 200 mg/kg group and 1000 ADG respectively (P0.05) and a significant increase in.2 (P0.01)) 1-14d. Compared with the control group, Enterococcus faecalis and diarrhea diarrhea index of 40 mg/kg group was significantly lower (P0.05). In 15-31d and 1-31d, compared with control group and antibiotics group, 40 Enterococcus faecalis and 1000 mg/kg group were decreased to a certain extent, the diarrhea rate (P0.05).3) compared with the control group, Enterococcus faecalis can increase in blood total Protein content, including 200 Enterococcus faecalis and 1000 mg/kg group reached a significant level (P0.01), and the two groups were significant (P0.05) and (P0.01) significantly improve globulin content. Compared with the antibiotic group, 200 Enterococcus faecalis and 1000 mg/kg significantly increased the total protein content (P0.05) and compared. Control group, antibiotic group and Enterococcus faecalis 1000 group mg/kg albumin and globulin content ratio (white ball ratio) significantly decreased (P0.05), in addition, the antibiotic group blood alanine aminotransferase and aspartate aminotransferase activity decreased significantly (P0.05), Enterococcus faecalis 200 group mg/kg ALT activity was significantly decreased (P0.05.4)) compared with the control group, adding Enterococcus faecalis in diet on Weaned Piglets serum malondialdehyde (MDA) content is not significantly affected (P0.05), and antibiotics can significantly decrease the content of serum MDA (P0.05) 1000. Enterococcus faecalis mg/kg group can significantly improve Gu Guanggan Glutathione peroxidase (GSH-PX) activity (P0.05), catalase (CAT) could improve the activity of the trend (P0.05); the 200 group mg/kg significantly increased CAT activity (P0.05), with the trend of increasing the activity of GSH-PX (P0.05); 40mg/kg group could significantly increase the activity of CAT (P0.05) compared with control group and antibiotics. Group 1000, Enterococcus faecalis mg/kg group could significantly increase serum superoxide dismutase (SOD) activity (P0.01), Enterococcus faecalis 200 mg/kg group has the trend of increasing the activity of SOD (P0.05).5) compared with the control group, Mg /kg group of 1000 Enterococcus faecalis liver weight and spleen index were increased (P0.05), compared with the antibiotic group, 200 Enterococcus faecalis and 1000 mg/kg group showed significant increase in liver weight (P0.05).6) compared with the control group, the diet containing 1000 mg/kg of Enterococcus faecalis can significantly improve ileal villus height and crypt depth ratio (V/C) (P0.05), and has provided High duodenal villus height and crypt depth of the lower ileum trend (P0.05); compared with the antibiotic group, the diet containing 1000 mg/kg of Enterococcus faecalis can significantly improve ileal V/C (P0.05), which has lower ileal crypt depth. (P0.05).7) antibiotics and Enterococcus faecalis in diet on the effective sequence of microflora the number of piglets weaned, operational taxonomic units (OTU) and a diversity index (Chao1 index, Shannon index, Ace index and Simpson index) had no significant effect (P0.05). Compared with the control group, antibiotics can be respectively improved to a certain extent and reduce the Proteobacteria and actinobacteria (the proportion of bacteria P0.05), Enterococcus faecalis 40 and 1000mg/kg group can reduce the proportion of Proteobacteria bacteria in a certain extent (P0.05). Compared with the antibiotic group, Enterococcus faecalis added 1000 mg/kg diet with high proportion of bacteria and reduce bacteria thick door Proteobacteria bacteria proportion trend (P.05), Enterococcus faecalis 40 mg/kg group can reduce the Proteobacteria bacteria trend (P0.05). Further from the family level analysis of microflora composition of the, compared with the control group, adding 1000 mg/kg of Enterococcus faecalis could significantly enhance spore bacilli and cecum of weaned piglets in the diet (P0.05), significantly reduced the clostridiaceae and streptococcaceae bacteria ratio (P0.05), lactic acid bacilli have increased trend (P0.05). The mg/kg group has increased 40 Enterococcus faecalis bacillus, bacteria and bacteria, reduce bacteria shuttle trend (P0.05). Compared with the control group, antibiotics increase the proportion of Enterobacteriaceae in a certain extent (P0.05), and Enterococcus faecalis can reduce Enterobacteriaceae in a certain extent, the ratio of (P0.05), and Enterococcus faecalis group and antibiotic group was significant difference (P0.05). The results showed that dietary Tim The growth performance and Enterococcus faecalis can improve weaned piglets (15-31d), reduce the diarrhea rate, improve immunity and antioxidant capacity in weaned piglets, promoting the development of small intestine morphology, optimization of microflora composition; in three concentration of Enterococcus faecalis, with dosage of 1000 mg/kg is better than the others.

【學位授予單位】：西南大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S828.5

【參考文獻】

相關期刊論文 前10條

1 貢筱;郭俊剛;吳學壯;劉志;高秀華;楊福合;邢秀梅;;飼糧中添加枯草芽孢桿菌和糞腸球菌對育成期藍狐生長性能、營養(yǎng)物質消化率及氮代謝的影響[J];動物營養(yǎng)學報;2014年04期

2 李濤;曾東;倪學勤;王志全;辛金鴿;沈雪嬌;;乳酸桿菌發(fā)酵飼料對豬生長性能和肉質及血清抗氧化性能的影響[J];湖南農業(yè)大學學報(自然科學版);2014年02期

3 周盟;張乃鋒;董曉麗;王黎文;屠焰;紀守坤;張立霞;崔祥;樓燦;刁其玉;;益生菌對斷奶仔豬生長性能、免疫器官指數(shù)及胃腸道pH的影響[J];動物營養(yǎng)學報;2014年02期

4 張董燕;季海峰;王四新;王晶;劉輝;王雅民;;豬源唾液乳桿菌對生長豬生長性能、糞中微生物數(shù)量及血清指標的影響[J];動物營養(yǎng)學報;2014年03期

5 王永;楊維仁;張桂國;;飼糧中添加屎腸球菌對斷奶仔豬生長性能、腸道菌群和免疫功能的影響[J];動物營養(yǎng)學報;2013年05期

6 董曉麗;張乃鋒;周盟;屠焰;刁其玉;;復合菌制劑對斷奶仔豬生長性能、糞便微生物和血清指標的影響[J];動物營養(yǎng)學報;2013年06期

7 喻婷;鄧兵;張金鳳;錢運國;王麗霞;梁運祥;洪博;周華;葉勝強;童偉文;冉志平;;飼料中添加丁酸梭菌、糞腸球菌對櫻桃谷鴨生長性能、屠宰性狀的影響[J];飼料工業(yè);2012年24期

8 劉輝;季海峰;張董燕;王四新;王晶;單達聰;王雅民;;飼糧添加短乳桿菌對生長豬生長性能和血清生化指標的影響[J];動物營養(yǎng)學報;2013年01期

9 林謙;戴求仲;賓石玉;蔣桂韜;張建華;王向榮;張旭;;益生菌與酶制劑對黃羽肉雞血液生化指標和免疫性能影響的協(xié)同效應研究[J];飼料工業(yè);2012年14期

10 辛娜;張乃鋒;刁其玉;周盟;;芽孢桿菌制劑對斷奶仔豬生長性能、胃腸道發(fā)育的影響[J];畜牧獸醫(yī)學報;2012年06期

相關博士學位論文 前3條

1 計堅;解淀粉芽孢桿菌SC06介導豬腸道免疫機理的研究[D];浙江大學;2013年

2 崔志文;鼠李糖乳桿菌影響仔豬腸道屏障功能的研究[D];浙江大學;2013年

3 黃怡;屎腸球菌EF1對仔豬小腸黏膜屏障功能的影響[D];浙江大學;2012年

相關碩士學位論文 前5條

1 張?zhí)礻?飼喂乳酸菌對生長育肥豬生長、胴體及肉質特性影響的研究[D];山東農業(yè)大學;2013年

2 鮑延娥;糞腸球菌益生特性的評價研究[D];西北農林科技大學;2013年

3 侯璐;豬源糞腸球菌的特性及對仔豬生長性能和免疫力影響的研究[D];內蒙古農業(yè)大學;2010年

4 毛倩;植物源性益生素對生長育肥豬生產性能、肉質、盲腸微生物及代謝產物的影響[D];四川農業(yè)大學;2009年

5 陳旭東;果寡糖和芽孢桿菌對斷奶仔豬生產性能的影響及其機理[D];中國農業(yè)大學;2004年

，

本文編號：1460027