本文關(guān)鍵詞： 奧爾森派琴蟲 PCR檢測 水產(chǎn)品 澳大利亞　出處：《中國獸醫(yī)科學(xué)》2017年05期 　論文類型：期刊論文

【摘要】：為快速、準(zhǔn)確地檢測進(jìn)出境水產(chǎn)品中的派琴蟲,防止有害寄生蟲傳入,對(duì)OIE推薦的奧爾森派琴蟲PCR檢測方法的擴(kuò)增程序進(jìn)行優(yōu)化,并應(yīng)用于上�？诎稄�16個(gè)國家進(jìn)口的21種貝類水產(chǎn)品95批次的奧爾森派琴蟲的檢疫。結(jié)果顯示,該優(yōu)化的PCR擴(kuò)增程序用時(shí)由原來的224 min縮短為91 min,效率提高了2.6倍,且最低檢測限相同;并從澳大利亞進(jìn)口的9個(gè)扇貝樣品中檢出7份奧爾森派琴蟲陽性,陽性率為77.8%。測序結(jié)果表明,與奧爾森派琴蟲序列(登錄號(hào)為U07701.1)的同源性為99%。試驗(yàn)證明優(yōu)化的PCR檢測方法快速、準(zhǔn)確,適用于進(jìn)出境水產(chǎn)品中奧爾森派琴蟲的檢疫,并提示在進(jìn)口貝類水產(chǎn)品中,應(yīng)加強(qiáng)澳大利亞進(jìn)口水產(chǎn)品的檢疫。
[Abstract]:In order to quickly and accurately detect Paiqin in aquatic products and prevent the introduction of harmful parasites, the amplification procedure of PCR detection method recommended by OIE was optimized. It was applied to the quarantine of 21 shellfish species imported from 16 countries in Shanghai Port. The results showed that the quarantine of Orson Peiqin insects was carried out in 95 batches of shellfish products from 16 countries. The time of the optimized PCR amplification program was shortened from 224 min to 91 min, the efficiency was increased 2.6 times, and the minimum detection limit was the same. In addition, 7 samples from 9 scallop samples from Australia were found to be positive, the positive rate was 77.8%. The sequencing results showed that the positive rate was 77.8%. The homology of the PCR sequence with that of Orson Peiqin (accession number U07701.1) was 990.The optimized PCR detection method was proved to be fast and accurate. It is suitable for the quarantine of Orson Phedrina in and out of aquatic products, and suggests that the quarantine of imported aquatic products of Australia should be strengthened in the imported shellfish.
【作者單位】： 上海出入境檢驗(yàn)檢疫局;
【基金】：國家重點(diǎn)研發(fā)計(jì)劃項(xiàng)目(2016YFC1202000,2016YFC1202004)
【分類號(hào)】：S851.347.34
【正文快照】： 派琴蟲(Perkinsus sp.)是寄生或者游離在貝類等軟體動(dòng)物結(jié)締組織、鰓、內(nèi)臟或套膜上皮中的一種重要的寄生蟲,可引起貝殼不能閉合、套膜收縮、性腺發(fā)育抑制、生長緩慢等,對(duì)海水養(yǎng)殖貝類造成Corresponding author:HE Yu-ping,E-mail:heyuping@shcig.gov.cn來源國家Origin樣品名，

本文編號(hào)：1448542