本文關(guān)鍵詞：敵草快和硫辛酸對(duì)育肥豬腸道結(jié)構(gòu)及消化功能的影響　出處：《動(dòng)物營(yíng)養(yǎng)學(xué)報(bào)》2016年10期 　論文類型：期刊論文

  更多相關(guān)文章： 敵草快 硫辛酸 育肥豬 氧化應(yīng)激 腸道

【摘要】：本文旨在研究敵草快和硫辛酸(LA)對(duì)育肥豬腸道結(jié)構(gòu)及消化功能的影響,以探究LA對(duì)敵草快引起的應(yīng)激是否有緩解作用。選取24頭(70.64±3.61)kg的健康大白閹公豬,按照2×2因子設(shè)計(jì),隨機(jī)分為對(duì)照組、LA組、敵草快組和LA+敵草快組,每個(gè)組設(shè)6個(gè)重復(fù),每個(gè)重復(fù)1頭豬。試驗(yàn)期29 d。LA添加劑量為800 mg/kg飼糧,飼喂貫穿試驗(yàn)全程,而一次性腹腔滴注敵草快于試驗(yàn)第15天進(jìn)行,無腹腔滴注敵草快試驗(yàn)豬滴注等量的生理鹽水。于試驗(yàn)第29天對(duì)所有試驗(yàn)豬前腔靜脈采血后屠宰取樣,運(yùn)用試劑盒檢測(cè)血漿和腸道氧化損傷標(biāo)志物的含量以及空腸食糜消化酶的活性,蘇木精-伊紅(HE)染色后運(yùn)用圖像分析軟件觀察腸道形態(tài)結(jié)構(gòu)并測(cè)定絨毛高度、隱窩深度,并計(jì)算絨毛高度/隱窩深度(V/C),熒光定量PCR法檢測(cè)閉合蛋白(occludin)和緊密結(jié)合蛋白(ZO-1)的相對(duì)表達(dá)量。結(jié)果表明:1)敵草快極顯著升高了試驗(yàn)豬血漿和腸道丙二醛(MDA)、蛋白質(zhì)羰基(PCO)和8-羥基鳥苷(8-OHd G)的含量(P0.01),極顯著降低了十二指腸、空腸及回腸絨毛高度、隱窩深度(P0.01),極顯著增大了十二指腸和空腸V/C(P0.01),顯著增大了回腸V/C(P0.05),極顯著降低了空腸occludin和ZO-1 m RNA相對(duì)表達(dá)量(P0.01),極顯著降低了空腸食糜中淀粉酶、胰蛋白酶以及脂肪酶的活性(P0.01)。2)飼糧中添加LA對(duì)試驗(yàn)豬血漿和腸道MDA、PCO和8-OHd G含量的影響均不顯著(P0.05),對(duì)十二指腸、空腸和回腸的絨毛高度、隱窩深度以及V/C的影響均不顯著(P0.05),對(duì)空腸occludin和ZO-1 m RNA相對(duì)表達(dá)量的影響也不顯著(P0.05),對(duì)空腸食糜中淀粉酶、胰蛋白酶以及脂肪酶活性的影響差異也均不顯著(P0.05)。3)在應(yīng)激狀態(tài)下,飼糧中添加LA使豬血漿8-OHd G含量顯著降低(P0.05),十二指腸絨毛高度、空腸絨毛高度以及回腸隱窩深度顯著升高(P0.05),空腸V/C顯著降低(P0.05),回腸V/C極顯著降低(P0.01),空腸occludin和ZO-1 m RNA相對(duì)表達(dá)量顯著升高(P0.05),空腸食糜淀粉酶和胰蛋白酶的活性顯著提升(P0.05)。由此可見,敵草快引起了育肥豬強(qiáng)烈的氧化應(yīng)激,導(dǎo)致了腸道結(jié)構(gòu)的嚴(yán)重?fù)p傷并減弱了腸道的消化功能;在敵草快引起的應(yīng)激狀態(tài)下,飼糧中添加800 mg/kg LA能夠減緩腸道結(jié)構(gòu)的損傷并能一定程度地提高腸道的消化功能。
[Abstract]:The purpose of this study was to study the effects of diphacinon and lipoxylic acid on intestinal structure and digestive function of fattening pigs. In order to find out whether LA can relieve the stress induced by dioxin, 24 healthy white castrated boars with 70.64 鹵3.61kg / kg were randomly divided into two groups according to the 2 脳 2 factor design. Each group was divided into 6 replicates and 1 pig per replica. During the trial period, the supplementation dose of 29 d.LA was 800 mg/kg diet and fed through the whole course of the experiment. However, once intraperitoneal drip of diphacia was faster than that of the 15th day of the experiment, and no intraperitoneal drip of the same amount of normal saline was given to the pigs. On the 29th day of the experiment, blood samples were collected from the anterior vena cava of all the tested pigs and slaughtered. The contents of plasma and intestinal oxidative damage markers and the activity of jejunum chyme digestive enzyme were detected by the kit. After hematoxylin and eosin (HEH) staining, the shape and structure of intestinal tract were observed by image analysis software, and the villi height and crypt depth were measured, and the villi height / crypt depth (V / C) were calculated. Fluorescence quantitative PCR method was used to detect the relative expression of closed protein (Occludin1) and compact binding protein (ZO-1). The plasma and intestinal malondialdehyde (MDAs) were significantly increased in pigs. The contents of protein carbonyl PCOand 8-hydroxyguanosine 8-OHd G) significantly decreased the height of duodenum, jejunum and ileum villi, and the depth of crypt (P0.01). Very significantly increased the duodenum and jejunum V / C ~ (1) P _ (0.01) and ileum (V _ (/ C) P _ (0.05)). The relative expression of occludin and ZO-1 m RNA in jejunum and the amylase in jejunum chyme were significantly decreased. The contents of PCO and 8-OHdG in plasma and intestinal tract of pigs were not significantly affected by the addition of LA to the diet of trypsin and lipase activity (P0.01U. 2). The effects of LA on the contents of PCO and 8-OHdG in plasma and intestinal tract of pigs were not significant (P 0.05). There was no significant effect on villus height, crypt depth and V / C in duodenum, jejunum and ileum (P 0.05). The relative expression of occludin and ZO-1 m RNA in jejunum was not significantly affected by P0.05, but amylase in jejunum chyme. There was no significant difference in trypsin and lipase activity between the two groups. Under the stress condition, the content of 8-OHdG in pig plasma was significantly decreased by adding LA in diet. The height of duodenal villi, the height of jejunum villi and the depth of ileum recess increased significantly (P 0.05), the jejunum V / C decreased significantly (P 0.05), and the ileum V / C decreased significantly (P 0.01). The relative expression of occludin and ZO-1 m RNA in jejunum increased significantly, while the activities of chyme amylase and trypsin in jejunum increased significantly. Diphacia caused severe oxidative stress in fattening pigs, which resulted in serious damage of intestinal structure and weakened intestinal digestive function. Under the stress induced by diphacinone, adding 800 mg/kg LA to diet could attenuate the damage of intestinal structure and improve the digestive function of intestinal tract to a certain extent.
【作者單位】： 中國(guó)農(nóng)業(yè)科學(xué)院北京畜牧獸醫(yī)研究所動(dòng)物營(yíng)養(yǎng)學(xué)國(guó)家重點(diǎn)實(shí)驗(yàn)室;河南科技大學(xué)動(dòng)物科技學(xué)院;
【基金】：國(guó)家重點(diǎn)基礎(chǔ)研究發(fā)展計(jì)劃課題(2012CB124706) 國(guó)家科技支撐計(jì)劃課題(2012BAD39B02) 中國(guó)農(nóng)業(yè)科學(xué)院科技創(chuàng)新工程(ASTIP-IAS07)
【分類號(hào)】：S828.5
【正文快照】： 生物體內(nèi)的能量代謝將氧氣作為有氧代謝過程中的電子接受體,不可避免地產(chǎn)生活性氧自由基(reactive oxygen species,ROS)和活性氮自由基(reactive nitrogen species,RNS),過量的ROS產(chǎn)生及引發(fā)的脂質(zhì)過氧化損傷及炎癥反應(yīng)可通過多種途徑導(dǎo)致氧化應(yīng)激狀態(tài)和疾病[1-2]。在氧化應(yīng)激

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