本文關(guān)鍵詞：柯爾克孜羊超數(shù)排卵、同期發(fā)情及胚胎移植效果研究　出處：《塔里木大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

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【摘要】：無論是對綿羊的超數(shù)排卵和同期發(fā)情,還是對綿羊的胚胎移植,雖然國內(nèi)外的諸多學(xué)者已經(jīng)研究了數(shù)十年,但在各種各樣的因素影響之下,取得的效果并不理想。在本研究中,以柯爾克孜綿羊作為研究對象,不僅詳細地分析了影響該種綿羊超排效果和同期發(fā)情的主要因素,而且深入地分析了影響其胚胎移植妊娠效果的關(guān)鍵因素,初步建立高寒牧區(qū)綿羊的胚胎移植技術(shù)體系。試驗結(jié)果表明:像阿合奇縣等高寒牧區(qū)進行綿羊超數(shù)排卵最佳時間為9月一10月中旬,但進入12月份后超排效果出現(xiàn)不太穩(wěn)定的趨勢;通過應(yīng)用PG+FSH法和CIDR+FSH法對柯爾克孜羊進行超排處理,即使平均的采胚數(shù)不存在顯著的差異,不過通過第2種方法計算出來的平均可用胚數(shù)出現(xiàn)一定的減小;在激素總量相同的情況下,從藥物成本來看,對柯爾克孜羊應(yīng)用PG十FSH法超排的效果優(yōu)于CIDR十FSH法;在每組的柯爾克孜羊中,都通過藥物進行作用,用量可以有210IU、250IU、270IU、320IU的FSH,實現(xiàn)超排的處理能夠發(fā)現(xiàn),每只作用250IU得到的效果最好,平均的采胚數(shù)和可用的胚數(shù),都比其他組別更加顯著(P0.01);對柯爾克孜羊首次超排后、間隔1個和2個情期超排的排卵數(shù)分別為9.55士2.45枚、6.20士0.84枚和6.71士0.61枚,差異不顯著(P0.05),但兩者與首次超排的差異顯著;對供體羊是否完成補飼過程進行對照可知,平均的采胚數(shù)不存在顯著的差異,不過經(jīng)過補飼的羊,相對那些沒有經(jīng)過補飼的羊,在平均可用胚數(shù)的表達方面,前者遠遠超過后者,因此認為提高對飼養(yǎng)的監(jiān)管,可以有助于提高胚胎的成功率;通過LH的作用之后,能夠增加排卵數(shù)(P0.05),不過平均的采胚數(shù)和可用的胚數(shù),無法超過沒有作用的組別,同時沒有完成受精過程的卵數(shù),也遠遠多于沒有作用的組別,綜上所述,LH的刺激無法促進不成熟卵泡的排卵,使得實現(xiàn)受精過程的卵數(shù)量減少,從而減少了可用胚數(shù);CIDR法與PG同期發(fā)情的效果相比較,雖然平均采胚數(shù)沒有顯著差異(P0.05),卻平均可用胚數(shù)明顯下降(P0.05);應(yīng)用PG和CIDR法對柯爾克孜羊進行同期發(fā)情都有較好的效果(P0.05),其中在高寒牧區(qū)使用CIDR+PMSG+PG法對處于非繁殖季節(jié)的柯爾克孜羊進行處理后的同期發(fā)情率、受胎率及產(chǎn)羔率最好;在相同發(fā)育階段的條件下移植一枚胚胎后,A級胚胎與B級胚胎的移植受胎率之間沒有顯著差異,B級胚胎與C級胚胎的移植受胎率相比較無顯著差異(P0.05),但從受胎率的變化情況看出,隨著胚胎質(zhì)量的下降其移植受胎率也出現(xiàn)明顯的下降趨勢(P0.05);當選擇發(fā)情同步差在0h、士12h的供、受體羊進行移植時,其受胎率顯著高于士24h移植受胎率,但選擇在0h、士12h兩個時間段內(nèi)進行移植時其差異不顯著(P0.05)。從上述結(jié)果分析可知,在沒有足夠的,發(fā)情同步的受體羊時,為了獲得較好的產(chǎn)羔率,選擇使用同步差在士12h之內(nèi)的受體羊是最好。
[Abstract]:Both the sheep superovulation and estrus, or sheep embryo transplantation, although many scholars at home and abroad have been studied for decades, but under the influence of various factors, the results are not ideal. In this study, the Kyrgyz sheep as research object, not only are analyzed in detail. The main factors affecting sheep superovulation and estrus synchronization, and in-depth analysis of the key factors influencing the effects of embryo transfer pregnancy, embryo transplantation technology system was established in alpine pasture sheep. The test results show: like Akeqi County alpine pastoral areas to sheep superovulation and the best time for September in mid October. But in December after superovulation appear less stable trend; the Kirghiz sheep were superovulated with the application of PG+FSH method and CIDR+FSH method, even if the average recovery embryo number does not exist A significant difference, but the average available embryos were calculated by second methods, the number will decrease; the total hormone in the same circumstances, from drug costs, better than the CIDR ten FSH of PG ten FSH application of Kirgiz sheep superovulation; in each group of kerc Zi sheep, through medication role, dosage can have 210IU, 250IU, 270IU, 320IU FSH, realize the processing over rows can be found, the best of each 250IU results, the average recovery of embryos and available embryos are more significant than other groups, (P0.01); first of Kirghiz sheep after superovulation, ovulation between 1 and 2. During the period of superovulation were 9.55 + 2.45, 6.20 + 0.84 and 6.71 + 0.61, the difference was not significant (P0.05), but the difference with the first superovulation significantly; the donor sheep feeding process is completed according to the average number of embryo collection deposit In a significant difference, but after feeding the sheep, relative to those without feeding the sheep, in the expression of the average number of available embryos, the former more than the latter, so that to improve the feeding regulation, can help to improve the success rate of embryo; through the LH function, can increase the number of ovulation (P0.05), but the average recovery of embryos and available embryos, not more than no group, and no number of fertilized eggs of more than not working groups, to sum up, LH stimulation can promote the immature follicle ovulation, which reduces the number of eggs to achieve fertilization process. Reduce the number of available embryos; CIDR and PG compared the effect of estrus, although the average recovery of embryos had no significant difference (P0.05), but the average number of available embryos decreased significantly (P0.05); the Kirgiz sheep estrus using PG and CIDR method Have a good effect (P0.05), estrus in the non breeding season of Kirghiz sheep which use CIDR+PMSG+PG in alpine pastoral area after the rate, pregnancy rate and best lambing rate; at the same developmental stage under the condition of embryo transplantation, there was no significant difference between grade B and grade a embryos embryo transfer pregnancy the rate of grade B and grade C, embryo embryo transfer pregnancy rate compared with no significant difference (P0.05), but the changes of pregnancy rate that decreased with the quality of embryo transfer pregnancy rate also significantly decreased (P0.05); when choosing the estrous synchronization difference in 0h + 12h for receptor the sheep were transplanted, the pregnancy rate was significantly higher than that of 24h pregnancy rate, but in 0h, 12h two time when transplantation no significant differences (P0.05). The analysis results from the above, there is not enough, estrus synchronization receptor Sheep, in order to obtain better lambing rate, choose to use the synchronization error within the 12h receptor in sheep, is the best.

【學(xué)位授予單位】：塔里木大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：S826

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