本文關(guān)鍵詞：固相萃取-超高效液相色譜-串聯(lián)質(zhì)譜法測定畜禽飲用水中23種抗生素和3種β-受體激動(dòng)劑　出處：《色譜》2016年02期 　論文類型：期刊論文

  更多相關(guān)文章： 超高效液相色譜-串聯(lián)質(zhì)譜 固相萃取 抗生素 β-受體激動(dòng)劑 畜禽飲用水

【摘要】：建立了固相萃取-超高效液相色譜-串聯(lián)質(zhì)譜同時(shí)測定畜禽飲用水中4類23種抗生素(磺胺類、喹諾酮類、四環(huán)素類、氯霉素類)和3種β-受體激動(dòng)劑(克倫特羅、萊克多巴胺、沙丁胺醇)的分析方法。樣品經(jīng)高氯酸調(diào)節(jié)pH至5.0,加入乙二胺四乙酸二鈉(Na2EDTA),HLB固相萃取柱富集,用超高效液相色譜-質(zhì)譜聯(lián)用儀分時(shí)段多反應(yīng)監(jiān)測離子模式分析,外標(biāo)法定量抗生素,內(nèi)標(biāo)法定量β-受體激動(dòng)劑。23種抗生素和3種β-受體激動(dòng)劑的空白基質(zhì)加標(biāo)回收率為50.7%~104.6%,相對標(biāo)準(zhǔn)偏差(n=3)為2.6%~8.8%。23種抗生素和3種β-受體激動(dòng)劑的線性關(guān)系良好,相關(guān)系數(shù)均大于0.994,檢出限為0.01~0.20 ng/L。通過該方法分析了北京地區(qū)36家規(guī)模化畜禽養(yǎng)殖場畜禽飲用水中的抗生素和β-受體激動(dòng)劑,檢出了多種抗生素,并對含量進(jìn)行了定量計(jì)算。
[Abstract]:A method for simultaneous determination of 23 antibiotics (sulfonamides, quinolones, tetracyclines) in drinking water of livestock and poultry by solid-phase extraction (SPE), ultra-high performance liquid chromatography-tandem mass spectrometry (SPLC-MS) was established. Chloramphenicol (chloramphenicol) and three 尾-receptor agonists (clenbuterol, ractopamine, salbutamol). The samples were adjusted to pH 5.0 by perchloric acid. HLB solid phase extraction column was used to enrich the samples by adding ethylenediamine tetraacetic acid sodium disodium (Na _ 2EDTAA). The ion pattern analysis was monitored by super-high performance liquid chromatography-mass spectrometry, and the antibiotics were quantified by external standard method. The recoveries of blank matrix were 50.7% and 104.6% for 尾 -agonist agonists and three 尾 -agonists respectively. The relative standard deviation (RSDs) was 2.68.8.23 antibiotics and 3 尾 -agonists, and the correlation coefficients were all greater than 0.994. The detection limit was 0. 01 ~ 0. 20 ng / L. The antibiotics and 尾-receptor agonists in drinking water of 36 large-scale livestock and poultry farms in Beijing area were analyzed by this method, and a variety of antibiotics were detected. The content was calculated quantitatively.
【作者單位】： 北京市畜牧業(yè)環(huán)境監(jiān)測站;
【基金】：北京市農(nóng)委基金項(xiàng)目(PXM2014 036240 000012)~~
【分類號】：S859.8;O657.63
【正文快照】： *通訊聯(lián)系人.E-mail:stoneao12@sohu.com.抗生素(antibiotics)又稱抗生菌,是微生物(包括細(xì)菌、真菌、放線菌屬)或高等動(dòng)植物在生活過程中產(chǎn)生的具有抗病原體或其他活性的一類次級代謝產(chǎn)物,能干擾其他生活細(xì)胞發(fā)育功能的化學(xué)物質(zhì)。自1928年英國科學(xué)家Alexander Fleming首次從青

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