【摘要】： HIV是人類的頑強(qiáng)對手,自從1981年發(fā)現(xiàn)艾滋病二十多年過去了,HIV疫苗研究雖然取得了重要進(jìn)展(一些候選疫苗在動物實驗中可部分阻斷HIV感染和延緩AIDS發(fā)生),但仍然沒有成功,HIV疫苗的研究對當(dāng)今科學(xué)提出了前所未有的挑戰(zhàn)。HIV具有高度變異性、潛伏感染性和免疫細(xì)胞靶向性,HIV引起的是慢性感染,在T細(xì)胞、巨噬細(xì)胞和單核細(xì)胞中建立病毒貯存庫,在這些細(xì)胞中部分病毒發(fā)生整合,以靜止的前病毒形式存在;病毒具有高度變異性,強(qiáng)大的免疫逃逸能力;免疫細(xì)胞本身就是病毒攻擊的靶細(xì)胞,由于存在誘導(dǎo)感染增強(qiáng)抗體的危險性,以及缺乏理想的感染動物模型等,這些都給AIDS疫苗的研制帶來了很大的困難。 本研究以中國HIV-1流行株保守區(qū)域Gag-Pol序列為目的基因,在密碼子優(yōu)化的基礎(chǔ)上,構(gòu)建了用于同源重組的穿梭質(zhì)粒PSC-11-GagPol,經(jīng)同源重組及篩選檢測,得到特異性表達(dá)HIV-1 Gag-Pol蛋白的重組痘苗病毒rMVA-Psc11-GagPol,經(jīng)過體液及細(xì)胞免疫結(jié)果的分析,對rMVA-Psc11-GagPol的免疫原性進(jìn)行了研究。
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圖片說明：HIV感染T細(xì)胞示意圖
[Abstract]:HIV is a tenacious opponent of human beings. More than 20 years after the discovery of AIDS in 1981, although some candidate vaccines can partially block HIV infection and delay the occurrence of AIDS in animal experiments, it is still not successful. HIV vaccine research poses an unprecedented challenge to today's science. HIV has a high degree of variability, latent infection and immune cell targeting. HIV causes chronic infection. Virus storage is established in T cells, macrophages and monocytes. Some of the viruses in these cells are integrated and exist in the form of static proviruses. The virus has a high degree of variability and strong immune escape ability, and the immune cells themselves are the target cells attacked by the virus. Due to the risk of inducing infection and enhancing antibodies, as well as the lack of an ideal animal model of infection, these bring great difficulties to the development of AIDS vaccine. In this study, the conserved region Gag-Pol sequence of Chinese HIV-1 epidemic strain was used as the target gene, and on the basis of codon optimization, the shuttle plasmid PSC-11-GagPol, for homologous recombination was constructed. The recombinant vaccinia virus rMVA-Psc11-GagPol, which specifically expressed HIV-1 Gag-Pol protein, was obtained by humoral and cellular immunity, and the immunogenicity of rMVA-Psc11-GagPol was studied.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2007
【分類號】：R392

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本文編號：2512410